single Cys2-His2 zinc finger domain of the GAGA protein flanked by basic residues is sufficient for high-affinity specific DNA binding

Specific DNA binding to the core consensus site GAGAGAG has been shown with an 82-residue peptide (residues 310-391) taken from the Drosophila transcription factor GAGA. Using a series of deletion mutants, it was demonstrated that the minimal domain required for specific binding (residues 310-372) i...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1996-04, Vol.93 (7), p.2822-2826
Hauptverfasser:	Pedone, P.V, Ghirlando, R, Clore, G.M, Gronenborn, A.M, Felsenfeld, G, Omichinski, J.G
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Amino acids Animals Base Sequence Binding Sites Biochemistry Cell Line Cloning, Molecular cysteine Deoxyribonucleic acid DNA DNA - metabolism DNA binding proteins DNA Primers DNA-binding domains Drosophila Drosophila - metabolism Drosophila melanogaster Drosophila Proteins Gels histidine Homeodomain Proteins - biosynthesis Homeodomain Proteins - chemistry Homeodomain Proteins - metabolism Insects Kinetics Molecular Sequence Data Oligonucleotides Peptide Fragments - chemistry Peptide Fragments - isolation & purification Peptide Fragments - metabolism Peptides Polymerase Chain Reaction - methods Promoter regions Protein Conformation Proteins Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - metabolism transcription factors Transcription Factors - biosynthesis Transcription Factors - chemistry Transcription Factors - metabolism Transfection Zinc Zinc Fingers
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!