Effects of Subtle Changes in the SU Protein of Ecotropic Murine Leukemia Virus on Its Brain Capillary Endothelial Cell Tropism and Interference Properties
PVC-211 murine leukemia virus (MuLV) is a neuropathogenic variant of Friend MuLV (F-MuLV) that causes a rapidly progressive neurodegenerative disease in susceptible rodents. PVC-211 MuLV, but not the parental F-MuLV, can infect rat brain capillary endothelial cells (BCEC) efficiently, and the major...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1996-01, Vol.215 (2), p.142-151 |
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| creator | MASUDA, MARI HANSON, CHARLOTTE A. ALVORD, W.GREGORY HOFFMAN, PAUL M. RUSCETTI, SANDRA K. MASUDA, MICHIAKI |
| description | PVC-211 murine leukemia virus (MuLV) is a neuropathogenic variant of Friend MuLV (F-MuLV) that causes a rapidly progressive neurodegenerative disease in susceptible rodents. PVC-211 MuLV, but not the parental F-MuLV, can infect rat brain capillary endothelial cells (BCEC) efficiently, and the major determinant for BCEC tropism of PVC-211 MuLV is localized within theXbaI–BamHI fragment of the viral genome containing the 5′ half of theenvgene. To further dissect theXbaI–BamHI region for its effects on BCEC tropism, we constructed recombinant viruses between PVC-211 MuLV and F-MuLV and tested their infectivity on a cell line established from rat BCEC. Our results indicated that Glu116-to-Gly and Glu129-to-Lys substitutions in the background of the F-MuLV envelope SU protein were sufficient for conferring BCEC tropism on the virus. Interference studies of these viruses on Rat-1 fibroblastic cells showed that the structure of the SU protein encoded by theXbaI–BamHI region also has significant effects on their affinity for the rat ecotropic MuLV receptor. These results support the possibility that structural elements I and II of the SU protein are important determinants for virus–receptor interaction. |
| doi_str_mv | 10.1006/viro.1996.0017 |
| format | Article |
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PVC-211 MuLV, but not the parental F-MuLV, can infect rat brain capillary endothelial cells (BCEC) efficiently, and the major determinant for BCEC tropism of PVC-211 MuLV is localized within theXbaI–BamHI fragment of the viral genome containing the 5′ half of theenvgene. To further dissect theXbaI–BamHI region for its effects on BCEC tropism, we constructed recombinant viruses between PVC-211 MuLV and F-MuLV and tested their infectivity on a cell line established from rat BCEC. Our results indicated that Glu116-to-Gly and Glu129-to-Lys substitutions in the background of the F-MuLV envelope SU protein were sufficient for conferring BCEC tropism on the virus. Interference studies of these viruses on Rat-1 fibroblastic cells showed that the structure of the SU protein encoded by theXbaI–BamHI region also has significant effects on their affinity for the rat ecotropic MuLV receptor. These results support the possibility that structural elements I and II of the SU protein are important determinants for virus–receptor interaction.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1996.0017</identifier><identifier>PMID: 8560761</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>3T3 Cells ; Amino Acid Sequence ; Animals ; Base Sequence ; Brain - virology ; Cell Line ; DNA, Viral ; Endothelium, Vascular - virology ; Fibroblasts - cytology ; Friend murine leukemia virus - chemistry ; Friend murine leukemia virus - genetics ; Friend murine leukemia virus - physiology ; Genetic Vectors ; Mice ; Molecular Sequence Data ; murine leukemia virus ; Proviruses - genetics ; Rats ; Receptors, Virus - metabolism ; Recombination, Genetic ; Retroviridae Proteins, Oncogenic - chemistry ; Retroviridae Proteins, Oncogenic - genetics ; Retroviridae Proteins, Oncogenic - physiology ; Structure-Activity Relationship ; Transduction, Genetic ; Viral Envelope Proteins - chemistry ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - physiology ; Viral Interference ; Virus Integration</subject><ispartof>Virology (New York, N.Y.), 1996-01, Vol.215 (2), p.142-151</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-dd3fbf44a67e1fa912637a6241b6e11f73aca25a985555401af2b8e384be9d9f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682296900173$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8560761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MASUDA, MARI</creatorcontrib><creatorcontrib>HANSON, CHARLOTTE A.</creatorcontrib><creatorcontrib>ALVORD, W.GREGORY</creatorcontrib><creatorcontrib>HOFFMAN, PAUL M.</creatorcontrib><creatorcontrib>RUSCETTI, SANDRA K.</creatorcontrib><creatorcontrib>MASUDA, MICHIAKI</creatorcontrib><title>Effects of Subtle Changes in the SU Protein of Ecotropic Murine Leukemia Virus on Its Brain Capillary Endothelial Cell Tropism and Interference Properties</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>PVC-211 murine leukemia virus (MuLV) is a neuropathogenic variant of Friend MuLV (F-MuLV) that causes a rapidly progressive neurodegenerative disease in susceptible rodents. PVC-211 MuLV, but not the parental F-MuLV, can infect rat brain capillary endothelial cells (BCEC) efficiently, and the major determinant for BCEC tropism of PVC-211 MuLV is localized within theXbaI–BamHI fragment of the viral genome containing the 5′ half of theenvgene. To further dissect theXbaI–BamHI region for its effects on BCEC tropism, we constructed recombinant viruses between PVC-211 MuLV and F-MuLV and tested their infectivity on a cell line established from rat BCEC. Our results indicated that Glu116-to-Gly and Glu129-to-Lys substitutions in the background of the F-MuLV envelope SU protein were sufficient for conferring BCEC tropism on the virus. Interference studies of these viruses on Rat-1 fibroblastic cells showed that the structure of the SU protein encoded by theXbaI–BamHI region also has significant effects on their affinity for the rat ecotropic MuLV receptor. These results support the possibility that structural elements I and II of the SU protein are important determinants for virus–receptor interaction.</description><subject>3T3 Cells</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Brain - virology</subject><subject>Cell Line</subject><subject>DNA, Viral</subject><subject>Endothelium, Vascular - virology</subject><subject>Fibroblasts - cytology</subject><subject>Friend murine leukemia virus - chemistry</subject><subject>Friend murine leukemia virus - genetics</subject><subject>Friend murine leukemia virus - physiology</subject><subject>Genetic Vectors</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>murine leukemia virus</subject><subject>Proviruses - genetics</subject><subject>Rats</subject><subject>Receptors, Virus - metabolism</subject><subject>Recombination, Genetic</subject><subject>Retroviridae Proteins, Oncogenic - chemistry</subject><subject>Retroviridae Proteins, Oncogenic - genetics</subject><subject>Retroviridae Proteins, Oncogenic - physiology</subject><subject>Structure-Activity Relationship</subject><subject>Transduction, Genetic</subject><subject>Viral Envelope Proteins - chemistry</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - physiology</subject><subject>Viral Interference</subject><subject>Virus Integration</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP1CAUx4nRrOPq1ZsJJ28dgbZQjtqMOskYTXbXK6H04aItVKCb-FX8tNLMxJuRC3l5f36PvB9CLynZU0L4mwcXw55KyfeEUPEI7SiRvCJ1Qx-jHSENq3jH2FP0LKXvpNRCkCt01bWcCE536PfBWjA54WDxzTrkCXB_r_03SNh5nO8B39zhLzFkKGXJHEzIMSzO4E9rdB7wCdYfMDuNv7q4FozHx0J7F3XJ93px06TjL3zwYyiwyekJ9zBN-HaDpBlrP-KjzxAtRPAGtlkLxOwgPUdPrJ4SvLjc1-ju_eG2_1idPn849m9PlWkEz9U41nawTaO5AGq1pIzXQnPW0IEDpVbU2mjWatm15TSEasuGDuquGUCO0tbX6PWZu8Twc4WU1eySKZ_UHsKalBCS81ay_wapIKzmpC3B_TloYkgpglVLdHPZg6JEbdbUZk1t1tRmrTx4dSGvwwzj3_hFU-l35z6UPTw4iCoZt61rdLHYU2Nw_0L_AdY2qGA</recordid><startdate>19960115</startdate><enddate>19960115</enddate><creator>MASUDA, MARI</creator><creator>HANSON, CHARLOTTE A.</creator><creator>ALVORD, W.GREGORY</creator><creator>HOFFMAN, PAUL M.</creator><creator>RUSCETTI, SANDRA K.</creator><creator>MASUDA, MICHIAKI</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960115</creationdate><title>Effects of Subtle Changes in the SU Protein of Ecotropic Murine Leukemia Virus on Its Brain Capillary Endothelial Cell Tropism and Interference Properties</title><author>MASUDA, MARI ; HANSON, CHARLOTTE A. ; ALVORD, W.GREGORY ; HOFFMAN, PAUL M. ; RUSCETTI, SANDRA K. ; MASUDA, MICHIAKI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-dd3fbf44a67e1fa912637a6241b6e11f73aca25a985555401af2b8e384be9d9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>3T3 Cells</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Brain - virology</topic><topic>Cell Line</topic><topic>DNA, Viral</topic><topic>Endothelium, Vascular - virology</topic><topic>Fibroblasts - cytology</topic><topic>Friend murine leukemia virus - chemistry</topic><topic>Friend murine leukemia virus - genetics</topic><topic>Friend murine leukemia virus - physiology</topic><topic>Genetic Vectors</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>murine leukemia virus</topic><topic>Proviruses - genetics</topic><topic>Rats</topic><topic>Receptors, Virus - metabolism</topic><topic>Recombination, Genetic</topic><topic>Retroviridae Proteins, Oncogenic - chemistry</topic><topic>Retroviridae Proteins, Oncogenic - genetics</topic><topic>Retroviridae Proteins, Oncogenic - physiology</topic><topic>Structure-Activity Relationship</topic><topic>Transduction, Genetic</topic><topic>Viral Envelope Proteins - chemistry</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - physiology</topic><topic>Viral Interference</topic><topic>Virus Integration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MASUDA, MARI</creatorcontrib><creatorcontrib>HANSON, CHARLOTTE A.</creatorcontrib><creatorcontrib>ALVORD, W.GREGORY</creatorcontrib><creatorcontrib>HOFFMAN, PAUL M.</creatorcontrib><creatorcontrib>RUSCETTI, SANDRA K.</creatorcontrib><creatorcontrib>MASUDA, MICHIAKI</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MASUDA, MARI</au><au>HANSON, CHARLOTTE A.</au><au>ALVORD, W.GREGORY</au><au>HOFFMAN, PAUL M.</au><au>RUSCETTI, SANDRA K.</au><au>MASUDA, MICHIAKI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Subtle Changes in the SU Protein of Ecotropic Murine Leukemia Virus on Its Brain Capillary Endothelial Cell Tropism and Interference Properties</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1996-01-15</date><risdate>1996</risdate><volume>215</volume><issue>2</issue><spage>142</spage><epage>151</epage><pages>142-151</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>PVC-211 murine leukemia virus (MuLV) is a neuropathogenic variant of Friend MuLV (F-MuLV) that causes a rapidly progressive neurodegenerative disease in susceptible rodents. PVC-211 MuLV, but not the parental F-MuLV, can infect rat brain capillary endothelial cells (BCEC) efficiently, and the major determinant for BCEC tropism of PVC-211 MuLV is localized within theXbaI–BamHI fragment of the viral genome containing the 5′ half of theenvgene. To further dissect theXbaI–BamHI region for its effects on BCEC tropism, we constructed recombinant viruses between PVC-211 MuLV and F-MuLV and tested their infectivity on a cell line established from rat BCEC. Our results indicated that Glu116-to-Gly and Glu129-to-Lys substitutions in the background of the F-MuLV envelope SU protein were sufficient for conferring BCEC tropism on the virus. Interference studies of these viruses on Rat-1 fibroblastic cells showed that the structure of the SU protein encoded by theXbaI–BamHI region also has significant effects on their affinity for the rat ecotropic MuLV receptor. These results support the possibility that structural elements I and II of the SU protein are important determinants for virus–receptor interaction.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8560761</pmid><doi>10.1006/viro.1996.0017</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1996-01, Vol.215 (2), p.142-151 |
| issn | 0042-6822 1096-0341 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 3T3 Cells Amino Acid Sequence Animals Base Sequence Brain - virology Cell Line DNA, Viral Endothelium, Vascular - virology Fibroblasts - cytology Friend murine leukemia virus - chemistry Friend murine leukemia virus - genetics Friend murine leukemia virus - physiology Genetic Vectors Mice Molecular Sequence Data murine leukemia virus Proviruses - genetics Rats Receptors, Virus - metabolism Recombination, Genetic Retroviridae Proteins, Oncogenic - chemistry Retroviridae Proteins, Oncogenic - genetics Retroviridae Proteins, Oncogenic - physiology Structure-Activity Relationship Transduction, Genetic Viral Envelope Proteins - chemistry Viral Envelope Proteins - genetics Viral Envelope Proteins - physiology Viral Interference Virus Integration |
| title | Effects of Subtle Changes in the SU Protein of Ecotropic Murine Leukemia Virus on Its Brain Capillary Endothelial Cell Tropism and Interference Properties |
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