Surface expression and ligand-based selection of cDNAs fused to filamentous phage gene VI

We describe a novel phage display system that affords the surface expression and hence affinity selection of cDNAs. The strategy is based on a new approach to functionally display proteins on filamentous phage through the attachment to the C-terminus of the minor coat protein VI. The utility of the...

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Veröffentlicht in:	Bio/Technology 1995-04, Vol.13 (4), p.378-382
Hauptverfasser:	Jespers, L.S. (Center for Molecular and Vascular Biology, Leuven, Belgium), Messens, J.H, De Keyser, A, Eeckhout, D, Brande, I. van den, Gansemans, Y.G, Lauwereys, M.J, Vlasuk, G.P, Stanssens, P.E
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ADN RECOMBINADO ADN RECOMBINE Agriculture alpha-Amylases - antagonists & inhibitors Amino Acid Sequence Ancylostoma - enzymology Ancylostoma - genetics ANCYLOSTOMA CANINUM Animals ANTIMETABOLITE ANTIMETABOLITES ANTIMETABOLITOS BACTERIOFAGOS BACTERIOPHAGE BACTERIOPHAGES Base Sequence Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Biotinylation Blotting, Western Capsid - genetics COAT PROTEINS COMPLEMENTARY DNA DNA DNA LIBRARIES DNA, Complementary - genetics ENZYME INHIBITORS EXPRESION GENICA EXPRESSION DES GENES Factor Xa - metabolism Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION Gene Library GENES Genetic engineering Genetic technics Genetic Vectors Humans INHIBIDORES DE ENZIMAS INHIBITEUR D'ENZYME ISOLATION Life Sciences Methods. Procedures. Technologies Molecular cloning Molecular Sequence Data Plant Proteins - genetics PROTEASAS PROTEASE PROTEASES PROTEINAS PROTEINASE INHIBITORS PROTEINE PROTEINS RECOMBINANT DNA Serine Endopeptidases - genetics Serine Proteinase Inhibitors - genetics SERINE PROTEINASES STRUCTURAL GENES Trypsin - metabolism Trypsin Inhibitors
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