Determination of 5-methylcytosine by acid hydrolysis of DNA with hydrofluoric acid

Quantitation of 5-methylcytosine in DNA after acid hydrolysis has been inaccurate because deamination of cytosine and 5-methylcytosine occurs during the hydrolysis procedure. There is little information in the literature regarding the use of hydrofluoric acid (HF) for DNA hydrolysis and we have ther...

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Veröffentlicht in:	Analytical biochemistry 1987-12, Vol.167 (2), p.347-351
Hauptverfasser:	Catania, J., Keenan, B.C., Margison, G.P., Fairweather, D.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Methylcytosine acid hydrolysis Analytical, structural and metabolic biochemistry Animals Base Composition Biological and medical sciences Chromatography, High Pressure Liquid Cytosine - analogs & derivatives Cytosine - analysis Deamination DNA - analysis Fundamental and applied biological sciences. Psychology HPLC Hydrofluoric Acid Hydrolysis kinetics Methylation Nucleic acids Nucleic bases, nucleotides
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