Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini
Rab11 p24 is a Ras-like small guanosine triphosphate (GTP)-binding protein, and specific antibodies against it were newly developed to explore its function. Using the antibody, Rab11 p24 was shown to be abundant in rat pancreas as well as in most rat tissues. To explore the involvement of Rab11 p24...
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| Veröffentlicht in: | Digestive diseases and sciences 1996, Vol.41 (1), p.133-138 |
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| container_title | Digestive diseases and sciences |
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| creator | HORI, Y TAKEYAMA, Y HIROYOSHI, M UEDA, T MAEDA, A OHYANAGI, H SAITOH, Y KAIBUCHI, K TAKAI, Y |
| description | Rab11 p24 is a Ras-like small guanosine triphosphate (GTP)-binding protein, and specific antibodies against it were newly developed to explore its function. Using the antibody, Rab11 p24 was shown to be abundant in rat pancreas as well as in most rat tissues. To explore the involvement of Rab11 p24 into the exocytotic process, the subcellular distribution of Rab11 p24 in rat pancreatic acini was evaluated also by use of the antibody. When the isolated acini were incubated with 1 x 10(-10) M cholecystokinin octapeptide (CCK-8) that induced the maximal stimulation, the amount of Rab11 p24 increased in the fractions of plasma membrane and zymogen granules, but decreased in the cytosol fraction. This redistribution was time-dependent and occurred within 1 min after the CCK-8 stimulation and reached a maximal level within 2 min after the stimulation. Moreover, a light microscopic immunolabeling technique on the isolated rat pancreatic acini also revealed that higher immunoreactivity with Rab11 p24 was observed over the zymogen granule membrane under CCK-8 stimulation. The present results indicate that Rab11 p24 is translocated from cytosol to the membrane fraction during stimulation with CCK-8 and suggest that Rab11 p24 is involved in the intracellular vesicular transport of isolated acini. |
| doi_str_mv | 10.1007/BF02208595 |
| format | Article |
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Using the antibody, Rab11 p24 was shown to be abundant in rat pancreas as well as in most rat tissues. To explore the involvement of Rab11 p24 into the exocytotic process, the subcellular distribution of Rab11 p24 in rat pancreatic acini was evaluated also by use of the antibody. When the isolated acini were incubated with 1 x 10(-10) M cholecystokinin octapeptide (CCK-8) that induced the maximal stimulation, the amount of Rab11 p24 increased in the fractions of plasma membrane and zymogen granules, but decreased in the cytosol fraction. This redistribution was time-dependent and occurred within 1 min after the CCK-8 stimulation and reached a maximal level within 2 min after the stimulation. Moreover, a light microscopic immunolabeling technique on the isolated rat pancreatic acini also revealed that higher immunoreactivity with Rab11 p24 was observed over the zymogen granule membrane under CCK-8 stimulation. The present results indicate that Rab11 p24 is translocated from cytosol to the membrane fraction during stimulation with CCK-8 and suggest that Rab11 p24 is involved in the intracellular vesicular transport of isolated acini.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1007/BF02208595</identifier><identifier>PMID: 8565746</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Biological Transport ; Cell Membrane - chemistry ; Cytoplasmic Granules - chemistry ; Cytosol - chemistry ; Enzyme Precursors ; Exocrine pancreas ; Exocytosis ; Female ; Fundamental and applied biological sciences. Psychology ; GTP-Binding Proteins - immunology ; GTP-Binding Proteins - metabolism ; Immunoblotting ; Immunohistochemistry ; In Vitro Techniques ; Male ; Mice ; Mice, Inbred BALB C ; Pancreas - drug effects ; Pancreas - metabolism ; rab GTP-Binding Proteins ; Rabbits ; Rats ; Rats, Wistar ; Sincalide - pharmacology ; Subcellular Fractions ; Tissue Distribution ; Vertebrates: digestive system</subject><ispartof>Digestive diseases and sciences, 1996, Vol.41 (1), p.133-138</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-9dd084fdc6d18a87a73f1445f2b4784eb76ba67a576f001ecaa5036e742fe833</citedby><cites>FETCH-LOGICAL-c311t-9dd084fdc6d18a87a73f1445f2b4784eb76ba67a576f001ecaa5036e742fe833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27921,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2985220$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8565746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HORI, Y</creatorcontrib><creatorcontrib>TAKEYAMA, Y</creatorcontrib><creatorcontrib>HIROYOSHI, M</creatorcontrib><creatorcontrib>UEDA, T</creatorcontrib><creatorcontrib>MAEDA, A</creatorcontrib><creatorcontrib>OHYANAGI, H</creatorcontrib><creatorcontrib>SAITOH, Y</creatorcontrib><creatorcontrib>KAIBUCHI, K</creatorcontrib><creatorcontrib>TAKAI, Y</creatorcontrib><title>Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><description>Rab11 p24 is a Ras-like small guanosine triphosphate (GTP)-binding protein, and specific antibodies against it were newly developed to explore its function. Using the antibody, Rab11 p24 was shown to be abundant in rat pancreas as well as in most rat tissues. To explore the involvement of Rab11 p24 into the exocytotic process, the subcellular distribution of Rab11 p24 in rat pancreatic acini was evaluated also by use of the antibody. When the isolated acini were incubated with 1 x 10(-10) M cholecystokinin octapeptide (CCK-8) that induced the maximal stimulation, the amount of Rab11 p24 increased in the fractions of plasma membrane and zymogen granules, but decreased in the cytosol fraction. This redistribution was time-dependent and occurred within 1 min after the CCK-8 stimulation and reached a maximal level within 2 min after the stimulation. Moreover, a light microscopic immunolabeling technique on the isolated rat pancreatic acini also revealed that higher immunoreactivity with Rab11 p24 was observed over the zymogen granule membrane under CCK-8 stimulation. The present results indicate that Rab11 p24 is translocated from cytosol to the membrane fraction during stimulation with CCK-8 and suggest that Rab11 p24 is involved in the intracellular vesicular transport of isolated acini.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Cell Membrane - chemistry</subject><subject>Cytoplasmic Granules - chemistry</subject><subject>Cytosol - chemistry</subject><subject>Enzyme Precursors</subject><subject>Exocrine pancreas</subject><subject>Exocytosis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GTP-Binding Proteins - immunology</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Pancreas - drug effects</subject><subject>Pancreas - metabolism</subject><subject>rab GTP-Binding Proteins</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sincalide - pharmacology</subject><subject>Subcellular Fractions</subject><subject>Tissue Distribution</subject><subject>Vertebrates: digestive system</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1r3DAQhkVJ2Gw-Lr0XdAg9hLiVbOvDxzR000KgoezdjOVRUCrLjuRd6F_Ir66yu2xBoJHmmZfhIeQjZ184Y-rrtxUrS6ZFIz6QJReqKkoh9QlZMi5zzbk8I-cpvTDGGsXlgiy0kELVcknensaUXOeRurAd_RYHDDMdLf0NHed0KutbCvmRCu_-IE0DeE8f1k9F50LvwjOd4jijC7d5Pp85gkHvNx4i3WJyZlfl35CmMe6CXRo9zNjTCYKJCLMzFIwL7pKcWvAJrw73BVmvvq_vfxSPvx5-3t89FqbifC6avme6tr2RPdegFajK8roWtuxqpWvslOxAKhBKWsY4GgDBKomqLi3qqrogn_exefPXDaa5HVx6XxoCjpvUKtUIXTGewZs9aGJ2FNG2U3QDxL8tZ-279_a_9wx_OqRuugH7I3oQnfvXhz4kA95mI8alI1Y2WuSo6h-g5orJ</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>HORI, Y</creator><creator>TAKEYAMA, Y</creator><creator>HIROYOSHI, M</creator><creator>UEDA, T</creator><creator>MAEDA, A</creator><creator>OHYANAGI, H</creator><creator>SAITOH, Y</creator><creator>KAIBUCHI, K</creator><creator>TAKAI, Y</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini</title><author>HORI, Y ; TAKEYAMA, Y ; HIROYOSHI, M ; UEDA, T ; MAEDA, A ; OHYANAGI, H ; SAITOH, Y ; KAIBUCHI, K ; TAKAI, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-9dd084fdc6d18a87a73f1445f2b4784eb76ba67a576f001ecaa5036e742fe833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Cell Membrane - chemistry</topic><topic>Cytoplasmic Granules - chemistry</topic><topic>Cytosol - chemistry</topic><topic>Enzyme Precursors</topic><topic>Exocrine pancreas</topic><topic>Exocytosis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GTP-Binding Proteins - immunology</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Pancreas - drug effects</topic><topic>Pancreas - metabolism</topic><topic>rab GTP-Binding Proteins</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sincalide - pharmacology</topic><topic>Subcellular Fractions</topic><topic>Tissue Distribution</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HORI, Y</creatorcontrib><creatorcontrib>TAKEYAMA, Y</creatorcontrib><creatorcontrib>HIROYOSHI, M</creatorcontrib><creatorcontrib>UEDA, T</creatorcontrib><creatorcontrib>MAEDA, A</creatorcontrib><creatorcontrib>OHYANAGI, H</creatorcontrib><creatorcontrib>SAITOH, Y</creatorcontrib><creatorcontrib>KAIBUCHI, K</creatorcontrib><creatorcontrib>TAKAI, Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HORI, Y</au><au>TAKEYAMA, Y</au><au>HIROYOSHI, M</au><au>UEDA, T</au><au>MAEDA, A</au><au>OHYANAGI, H</au><au>SAITOH, Y</au><au>KAIBUCHI, K</au><au>TAKAI, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini</atitle><jtitle>Digestive diseases and sciences</jtitle><addtitle>Dig Dis Sci</addtitle><date>1996</date><risdate>1996</risdate><volume>41</volume><issue>1</issue><spage>133</spage><epage>138</epage><pages>133-138</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>Rab11 p24 is a Ras-like small guanosine triphosphate (GTP)-binding protein, and specific antibodies against it were newly developed to explore its function. Using the antibody, Rab11 p24 was shown to be abundant in rat pancreas as well as in most rat tissues. To explore the involvement of Rab11 p24 into the exocytotic process, the subcellular distribution of Rab11 p24 in rat pancreatic acini was evaluated also by use of the antibody. When the isolated acini were incubated with 1 x 10(-10) M cholecystokinin octapeptide (CCK-8) that induced the maximal stimulation, the amount of Rab11 p24 increased in the fractions of plasma membrane and zymogen granules, but decreased in the cytosol fraction. This redistribution was time-dependent and occurred within 1 min after the CCK-8 stimulation and reached a maximal level within 2 min after the stimulation. Moreover, a light microscopic immunolabeling technique on the isolated rat pancreatic acini also revealed that higher immunoreactivity with Rab11 p24 was observed over the zymogen granule membrane under CCK-8 stimulation. 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| ispartof | Digestive diseases and sciences, 1996, Vol.41 (1), p.133-138 |
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| subjects | Animals Antibodies, Monoclonal Biological and medical sciences Biological Transport Cell Membrane - chemistry Cytoplasmic Granules - chemistry Cytosol - chemistry Enzyme Precursors Exocrine pancreas Exocytosis Female Fundamental and applied biological sciences. Psychology GTP-Binding Proteins - immunology GTP-Binding Proteins - metabolism Immunoblotting Immunohistochemistry In Vitro Techniques Male Mice Mice, Inbred BALB C Pancreas - drug effects Pancreas - metabolism rab GTP-Binding Proteins Rabbits Rats Rats, Wistar Sincalide - pharmacology Subcellular Fractions Tissue Distribution Vertebrates: digestive system |
| title | Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini |
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