DNA sequence amplification in human prostate cancer identified by chromosome microdissection: potential prognostic implications
The primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification) in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence in situ hybridization) were combined to (a) identi...
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| Veröffentlicht in: | Clinical cancer research 1995-01, Vol.1 (1), p.11-18 |
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| creator | VAN DEN BERG, C XIN-YUAN GUAN EPSTEIN, J SAROSDY, M MELTZER, P TRENT, J VON HOFF, D JENKINS, R BITTNER, M GRIFFIN, C KALLIONIEMI, O VISAKORPI, T MCGILL, J HERATH, J |
| description | The primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification)
in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence
in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b)
evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results
of chromosome microdissection revealed a common amplified band region (8q24.1-24. 2) in two prostate cases with cytological
evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was
performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences
from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node
at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization
and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease.
In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating
8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic
utility as a marker of prostate cancer progression. |
| format | Article |
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in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence
in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b)
evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results
of chromosome microdissection revealed a common amplified band region (8q24.1-24. 2) in two prostate cases with cytological
evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was
performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences
from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node
at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization
and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease.
In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating
8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic
utility as a marker of prostate cancer progression.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>PMID: 9815882</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Biological and medical sciences ; Chromosome Banding ; Chromosome Mapping ; Chromosomes, Human, Pair 8 ; DNA, Neoplasm - genetics ; Gene Amplification ; Humans ; In Situ Hybridization, Fluorescence ; Lymphatic Metastasis ; Male ; Medical sciences ; Neoplasm Staging ; Nephrology. Urinary tract diseases ; Ploidies ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - pathology ; Prostatic Neoplasms - surgery ; Tumor Cells, Cultured ; Tumors of the urinary system ; Urinary tract. Prostate gland</subject><ispartof>Clinical cancer research, 1995-01, Vol.1 (1), p.11-18</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3609358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9815882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VAN DEN BERG, C</creatorcontrib><creatorcontrib>XIN-YUAN GUAN</creatorcontrib><creatorcontrib>EPSTEIN, J</creatorcontrib><creatorcontrib>SAROSDY, M</creatorcontrib><creatorcontrib>MELTZER, P</creatorcontrib><creatorcontrib>TRENT, J</creatorcontrib><creatorcontrib>VON HOFF, D</creatorcontrib><creatorcontrib>JENKINS, R</creatorcontrib><creatorcontrib>BITTNER, M</creatorcontrib><creatorcontrib>GRIFFIN, C</creatorcontrib><creatorcontrib>KALLIONIEMI, O</creatorcontrib><creatorcontrib>VISAKORPI, T</creatorcontrib><creatorcontrib>MCGILL, J</creatorcontrib><creatorcontrib>HERATH, J</creatorcontrib><title>DNA sequence amplification in human prostate cancer identified by chromosome microdissection: potential prognostic implications</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>The primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification)
in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence
in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b)
evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results
of chromosome microdissection revealed a common amplified band region (8q24.1-24. 2) in two prostate cases with cytological
evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was
performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences
from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node
at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization
and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease.
In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating
8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic
utility as a marker of prostate cancer progression.</description><subject>Biological and medical sciences</subject><subject>Chromosome Banding</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 8</subject><subject>DNA, Neoplasm - genetics</subject><subject>Gene Amplification</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neoplasm Staging</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Ploidies</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Prostatic Neoplasms - surgery</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9LxDAQxYso67r6EYQc1FuhTZo09basf2HRi57LNJ1uI01bkxbZk1_dlBaZwwzMjzfz3kmwjjlPQ0YFP_VzlMowShg9Dy6c-4qiOImjZBWsMhlzKek6-H142xKH3yO2CgmYvtGVVjDoriW6JfVooCW97dwAAxIFnrJEl9gOnsOSFEeiatuZznUGidHKdqV2DtWkcE_6bphQaCaNQ-tltCJ6ujLfcJfBWQWNw6ulb4LPp8eP3Uu4f39-3W33Ye2dDKFIgUaU0YSlTDBVCcxKBQUFKXjBeZkgysQbBQ4ShFBJJKjMUlZwSWPKFNsEd7Ou_8ObdUNutFPYNNBiN7o8TTOe8FR48HoBx8JgmfdWG7DHfEnM72-WPTgFTWV9JNr9Y0xEGePSY7czVutD_aMt5nN2Fh2CVXUeTxWzPwtBhOk</recordid><startdate>19950101</startdate><enddate>19950101</enddate><creator>VAN DEN BERG, C</creator><creator>XIN-YUAN GUAN</creator><creator>EPSTEIN, J</creator><creator>SAROSDY, M</creator><creator>MELTZER, P</creator><creator>TRENT, J</creator><creator>VON HOFF, D</creator><creator>JENKINS, R</creator><creator>BITTNER, M</creator><creator>GRIFFIN, C</creator><creator>KALLIONIEMI, O</creator><creator>VISAKORPI, T</creator><creator>MCGILL, J</creator><creator>HERATH, J</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19950101</creationdate><title>DNA sequence amplification in human prostate cancer identified by chromosome microdissection: potential prognostic implications</title><author>VAN DEN BERG, C ; XIN-YUAN GUAN ; EPSTEIN, J ; SAROSDY, M ; MELTZER, P ; TRENT, J ; VON HOFF, D ; JENKINS, R ; BITTNER, M ; GRIFFIN, C ; KALLIONIEMI, O ; VISAKORPI, T ; MCGILL, J ; HERATH, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-67a20232437363cf6e9dcab2a865b55d4ee84043a5a8a66c40628973b582123c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Biological and medical sciences</topic><topic>Chromosome Banding</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 8</topic><topic>DNA, Neoplasm - genetics</topic><topic>Gene Amplification</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neoplasm Staging</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Ploidies</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Prostatic Neoplasms - surgery</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VAN DEN BERG, C</creatorcontrib><creatorcontrib>XIN-YUAN GUAN</creatorcontrib><creatorcontrib>EPSTEIN, J</creatorcontrib><creatorcontrib>SAROSDY, M</creatorcontrib><creatorcontrib>MELTZER, P</creatorcontrib><creatorcontrib>TRENT, J</creatorcontrib><creatorcontrib>VON HOFF, D</creatorcontrib><creatorcontrib>JENKINS, R</creatorcontrib><creatorcontrib>BITTNER, M</creatorcontrib><creatorcontrib>GRIFFIN, C</creatorcontrib><creatorcontrib>KALLIONIEMI, O</creatorcontrib><creatorcontrib>VISAKORPI, T</creatorcontrib><creatorcontrib>MCGILL, J</creatorcontrib><creatorcontrib>HERATH, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VAN DEN BERG, C</au><au>XIN-YUAN GUAN</au><au>EPSTEIN, J</au><au>SAROSDY, M</au><au>MELTZER, P</au><au>TRENT, J</au><au>VON HOFF, D</au><au>JENKINS, R</au><au>BITTNER, M</au><au>GRIFFIN, C</au><au>KALLIONIEMI, O</au><au>VISAKORPI, T</au><au>MCGILL, J</au><au>HERATH, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequence amplification in human prostate cancer identified by chromosome microdissection: potential prognostic implications</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>1995-01-01</date><risdate>1995</risdate><volume>1</volume><issue>1</issue><spage>11</spage><epage>18</epage><pages>11-18</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>The primary aim of this report was to examine the significance of increased DNA sequence copy number (gene amplification)
in human prostate cancers. Three methodologies (chromosome microdissection, comparative genomic hybridization, and fluorescence
in situ hybridization) were combined to (a) identify a common region of gene amplification in human prostate cells and (b)
evaluate in patient samples the prevalence of this genetic change in both primary and recurrent prostate samples. The results
of chromosome microdissection revealed a common amplified band region (8q24.1-24. 2) in two prostate cases with cytological
evidence of gene amplification (double minutes). Fluorescence in situ hybridization using the 8q microdissection probe was
performed on fresh tumor touch preparations from 44 randomly selected prostatectomy specimens. Amplification of DNA sequences
from 8q24 was observed in 4 (9%) of 44 cases. Four of the 44 patients in this series presented with a positive lymph node
at initial diagnosis and 3 of these 4 patients showed 8q amplification. Because of this finding, comparative genomic hybridization
and fluorescence in situ hybridization were performed on tumor cells from nine prostate cancer patients with recurrent disease.
In eight of nine cases a gain of DNA sequences encompassing 8q24 was observed. Taken together with other evidence implicating
8q gain in prostate cancer progression, these results suggest that the analysis of this genetic change may have diagnostic
utility as a marker of prostate cancer progression.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>9815882</pmid><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Association for Cancer Research; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 8 DNA, Neoplasm - genetics Gene Amplification Humans In Situ Hybridization, Fluorescence Lymphatic Metastasis Male Medical sciences Neoplasm Staging Nephrology. Urinary tract diseases Ploidies Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Prostatic Neoplasms - surgery Tumor Cells, Cultured Tumors of the urinary system Urinary tract. Prostate gland |
| title | DNA sequence amplification in human prostate cancer identified by chromosome microdissection: potential prognostic implications |
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