Rapid isolation of DNA from Actinomyces

DNA could not be quickly extracted from members of the genus Actinomyces by the usual methods of lysis. Treatment of 7 different actinomyces cells with lysozyme and achromopeptidase, both 5 mg/g wet cells, for 2 h, followed by SDS (0.2 %), proteinase K (5 mg/g wet cells) and EDTA (lmM) for 1 h, lyse...

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