Inhibition of herpes simplex virus DNA replication by ara-tubercidin
Preliminary studies of the biochemical basis for the antiviral activity of the pyrrolo[2,3- d]pyrimidine nucleoside ara-tubercidin were conducted. Herpes simplex virus DNA synthesis was 3-fold more sensitive to inhibition by ara-tubercidin than was cellular DNA synthesis. Partially purified herpes D...
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| Veröffentlicht in: | Antiviral research 1987-09, Vol.8 (2), p.97-102 |
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| container_end_page | 102 |
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| container_issue | 2 |
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| container_title | Antiviral research |
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| creator | Turk, Steven R. Dan Cook, P. Reinke, C.Michael Drach, John C. |
| description | Preliminary studies of the biochemical basis for the antiviral activity of the pyrrolo[2,3-
d]pyrimidine nucleoside ara-tubercidin were conducted. Herpes simplex virus DNA synthesis was 3-fold more sensitive to inhibition by ara-tubercidin than was cellular DNA synthesis. Partially purified herpes DNA polymerases were more sensitive to inhibition by ara-tubercidin 5′-triphosphate than were cellular polymerases α and β. Inhibition of viral DNA polymerase was competitive with dATP and noncompetitive with dTTP. The results suggest that the viral DNA polymerase plays a significant role in the antiviral activity of ara-tubercidin. |
| doi_str_mv | 10.1016/0166-3542(87)90080-5 |
| format | Article |
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d]pyrimidine nucleoside ara-tubercidin were conducted. Herpes simplex virus DNA synthesis was 3-fold more sensitive to inhibition by ara-tubercidin than was cellular DNA synthesis. Partially purified herpes DNA polymerases were more sensitive to inhibition by ara-tubercidin 5′-triphosphate than were cellular polymerases α and β. Inhibition of viral DNA polymerase was competitive with dATP and noncompetitive with dTTP. The results suggest that the viral DNA polymerase plays a significant role in the antiviral activity of ara-tubercidin.</description><subject>Action of physical and chemical agents</subject><subject>Antiviral</subject><subject>Antiviral Agents - pharmacology</subject><subject>Ara-tubercidin</subject><subject>Biological and medical sciences</subject><subject>DNA Replication - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpes</subject><subject>HSV</subject><subject>Humans</subject><subject>KB Cells</subject><subject>Kinetics</subject><subject>Microbiology</subject><subject>Nucleic Acid Synthesis Inhibitors</subject><subject>Nucleoside</subject><subject>Pyrrolopyrimidine</subject><subject>Simplexvirus - drug effects</subject><subject>Simplexvirus - genetics</subject><subject>Virology</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxEAQRRtRdHz8gUIWIrqIVjrp10aQ8TUw6EbXTae7gi2ZJHYnon9v5sEsXRS1qHMvxSHkNIPrDDJ-Mw5Pc1bQSymuFICElO2QSSYFTRUovksmW-SAHMb4CQBcKLlP9qnMQRZiQu5nzYcvfe_bJmmr5ANDhzGJftHV-JN8-zDE5P7lLgnY1d6aFVf-JiaYtB9KDNY73xyTvcrUEU82-4i8Pz68TZ_T-evTbHo3T22R0z7NbSlAKYbM5CXlvHDcGeUoViCl5bTELOdVzjMDFZUUaWmoUZYrdIwWAvMjcrHu7UL7NWDs9cJHi3VtGmyHqIVQDFgBI1isQRvaGANWugt-YcKvzkAv5emlGb00o6XQK3majbGzTf9QLtBtQxtb4_18czfRmroKprE-bjHBQHLIRux2jeHo4ttj0NF6bCw6H9D22rX-_z_-AG3rikQ</recordid><startdate>19870901</startdate><enddate>19870901</enddate><creator>Turk, Steven R.</creator><creator>Dan Cook, P.</creator><creator>Reinke, C.Michael</creator><creator>Drach, John C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870901</creationdate><title>Inhibition of herpes simplex virus DNA replication by ara-tubercidin</title><author>Turk, Steven R. ; Dan Cook, P. ; Reinke, C.Michael ; Drach, John C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-3cb70995e5a3b2664d6da9d2ef088c62be136f361a0f282e2ba2a9c69ed5247e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Action of physical and chemical agents</topic><topic>Antiviral</topic><topic>Antiviral Agents - pharmacology</topic><topic>Ara-tubercidin</topic><topic>Biological and medical sciences</topic><topic>DNA Replication - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpes</topic><topic>HSV</topic><topic>Humans</topic><topic>KB Cells</topic><topic>Kinetics</topic><topic>Microbiology</topic><topic>Nucleic Acid Synthesis Inhibitors</topic><topic>Nucleoside</topic><topic>Pyrrolopyrimidine</topic><topic>Simplexvirus - drug effects</topic><topic>Simplexvirus - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Turk, Steven R.</creatorcontrib><creatorcontrib>Dan Cook, P.</creatorcontrib><creatorcontrib>Reinke, C.Michael</creatorcontrib><creatorcontrib>Drach, John C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Antiviral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Turk, Steven R.</au><au>Dan Cook, P.</au><au>Reinke, C.Michael</au><au>Drach, John C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of herpes simplex virus DNA replication by ara-tubercidin</atitle><jtitle>Antiviral research</jtitle><addtitle>Antiviral Res</addtitle><date>1987-09-01</date><risdate>1987</risdate><volume>8</volume><issue>2</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>0166-3542</issn><eissn>1872-9096</eissn><coden>ARSRDR</coden><abstract>Preliminary studies of the biochemical basis for the antiviral activity of the pyrrolo[2,3-
d]pyrimidine nucleoside ara-tubercidin were conducted. Herpes simplex virus DNA synthesis was 3-fold more sensitive to inhibition by ara-tubercidin than was cellular DNA synthesis. Partially purified herpes DNA polymerases were more sensitive to inhibition by ara-tubercidin 5′-triphosphate than were cellular polymerases α and β. Inhibition of viral DNA polymerase was competitive with dATP and noncompetitive with dTTP. The results suggest that the viral DNA polymerase plays a significant role in the antiviral activity of ara-tubercidin.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2830847</pmid><doi>10.1016/0166-3542(87)90080-5</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-3542 |
| ispartof | Antiviral research, 1987-09, Vol.8 (2), p.97-102 |
| issn | 0166-3542 1872-9096 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Action of physical and chemical agents Antiviral Antiviral Agents - pharmacology Ara-tubercidin Biological and medical sciences DNA Replication - drug effects Fundamental and applied biological sciences. Psychology Herpes HSV Humans KB Cells Kinetics Microbiology Nucleic Acid Synthesis Inhibitors Nucleoside Pyrrolopyrimidine Simplexvirus - drug effects Simplexvirus - genetics Virology |
| title | Inhibition of herpes simplex virus DNA replication by ara-tubercidin |
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