Identification of Angiostrongylus cantonensis in infected rats with a coagglutination assay

The presence of Angiostrongylus cantonensis excretory secretory (ES) products was detected in the cerebrospinal fluid (CSF) of infected rats using a coagglutination assay. There was clear agglutination in 100% of CSF samples tested of the rats infected with third-stage larvae of A. cantonensis and n...

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Veröffentlicht in:	Southeast Asian journal of tropical medicine and public health 1995-12, Vol.26 (4), p.716-718
Hauptverfasser:	Rojas Rivero, L, Fonte, G L, Finlay, V C
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglutination Tests - methods Angiostrongylus cantonensis - immunology Animals Antigens, Helminth - cerebrospinal fluid Biological Assay Disease Models, Animal Rabbits Rats - parasitology Reproducibility of Results Sensitivity and Specificity Strongylida Infections - cerebrospinal fluid Strongylida Infections - immunology
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Zusammenfassung:	The presence of Angiostrongylus cantonensis excretory secretory (ES) products was detected in the cerebrospinal fluid (CSF) of infected rats using a coagglutination assay. There was clear agglutination in 100% of CSF samples tested of the rats infected with third-stage larvae of A. cantonensis and neither of the 20 non-infected rats produced agglutination. The assay detected 250 ng/ml or more of ES products. This assay is simple, easy to perform with minimum training and requires no equipment.
ISSN:	0125-1562