Influence of specimen treatment on nonreactive HTLV-III SERA
We investigated effects of specimen treatment on HTLV-III antibody assay results. Freezing and thawing specimens 10 times did not increase specimen mean absorbance (A) values measured by the Abbott immunoenzymometric assay (IEMA). Heating blood donor serum specimens at 56 degrees C for at least 10 m...
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| Veröffentlicht in: | AIDS research and human retroviruses 1987, Vol.3 (3), p.333-340 |
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| creator | MCBRIDE, J. H HOWANITZ, P. J RODGERSON, D. O MILES, J PETER, J. B |
| description | We investigated effects of specimen treatment on HTLV-III antibody assay results. Freezing and thawing specimens 10 times did not increase specimen mean absorbance (A) values measured by the Abbott immunoenzymometric assay (IEMA). Heating blood donor serum specimens at 56 degrees C for at least 10 min inactivates virus, but converted 7.1% of nonreactive specimens to positive when measured by this IEMA. Also heating at 56 degrees C for 30 min increased A values of most specimens; however, the conversion to positive within assay ranged from 0 to 75%, with a mean of 17%. When 56 nonreactive patient specimens were heated, 55% became Abbott IEMA reactive, although ENI IEMA values did not increase and Western blot results remained nonreactive. Binding of immunoglobulins G and A to Abbott IEMA beads did not cause this conversion after heat treatment. We postulate that heating specimens causes alteration of some serum matrices and introduces nonspecific binding to assay bead antigen or antibody reagents. We recommend that for the Abbott IEMA, specimens may be stored frozen, and must not be heated to avoid altered reactivity. |
| doi_str_mv | 10.1089/aid.1987.3.333 |
| format | Article |
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H ; HOWANITZ, P. J ; RODGERSON, D. O ; MILES, J ; PETER, J. B</creator><creatorcontrib>MCBRIDE, J. H ; HOWANITZ, P. J ; RODGERSON, D. O ; MILES, J ; PETER, J. B</creatorcontrib><description>We investigated effects of specimen treatment on HTLV-III antibody assay results. Freezing and thawing specimens 10 times did not increase specimen mean absorbance (A) values measured by the Abbott immunoenzymometric assay (IEMA). Heating blood donor serum specimens at 56 degrees C for at least 10 min inactivates virus, but converted 7.1% of nonreactive specimens to positive when measured by this IEMA. Also heating at 56 degrees C for 30 min increased A values of most specimens; however, the conversion to positive within assay ranged from 0 to 75%, with a mean of 17%. When 56 nonreactive patient specimens were heated, 55% became Abbott IEMA reactive, although ENI IEMA values did not increase and Western blot results remained nonreactive. Binding of immunoglobulins G and A to Abbott IEMA beads did not cause this conversion after heat treatment. We postulate that heating specimens causes alteration of some serum matrices and introduces nonspecific binding to assay bead antigen or antibody reagents. We recommend that for the Abbott IEMA, specimens may be stored frozen, and must not be heated to avoid altered reactivity.</description><identifier>ISSN: 0889-2229</identifier><identifier>EISSN: 1931-8405</identifier><identifier>DOI: 10.1089/aid.1987.3.333</identifier><identifier>PMID: 3325086</identifier><identifier>CODEN: ARHRE7</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>AIDS/HIV ; Antibodies, Viral - analysis ; Biological and medical sciences ; Blood Specimen Collection - methods ; Fundamental and applied biological sciences. 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H</creatorcontrib><creatorcontrib>HOWANITZ, P. J</creatorcontrib><creatorcontrib>RODGERSON, D. O</creatorcontrib><creatorcontrib>MILES, J</creatorcontrib><creatorcontrib>PETER, J. B</creatorcontrib><title>Influence of specimen treatment on nonreactive HTLV-III SERA</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>We investigated effects of specimen treatment on HTLV-III antibody assay results. Freezing and thawing specimens 10 times did not increase specimen mean absorbance (A) values measured by the Abbott immunoenzymometric assay (IEMA). Heating blood donor serum specimens at 56 degrees C for at least 10 min inactivates virus, but converted 7.1% of nonreactive specimens to positive when measured by this IEMA. Also heating at 56 degrees C for 30 min increased A values of most specimens; however, the conversion to positive within assay ranged from 0 to 75%, with a mean of 17%. When 56 nonreactive patient specimens were heated, 55% became Abbott IEMA reactive, although ENI IEMA values did not increase and Western blot results remained nonreactive. Binding of immunoglobulins G and A to Abbott IEMA beads did not cause this conversion after heat treatment. We postulate that heating specimens causes alteration of some serum matrices and introduces nonspecific binding to assay bead antigen or antibody reagents. We recommend that for the Abbott IEMA, specimens may be stored frozen, and must not be heated to avoid altered reactivity.</description><subject>AIDS/HIV</subject><subject>Antibodies, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Blood Specimen Collection - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIV - immunology</subject><subject>HIV Antibodies</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Microbiology</subject><subject>Predictive Value of Tests</subject><subject>Reagent Kits, Diagnostic</subject><subject>Techniques used in virology</subject><subject>Temperature</subject><subject>Virology</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFLwzAUh4Moc06v3oQexFtrkpe0KXgZY7rCQNDpNWTpK1TadDad4H9vxsqunt57vO_3O3yE3DKaMKryR1OXCctVlkACAGdkynJgsRJUnpMpVSqPOef5Jbny_otSmnMuJ2QCwCVV6ZQ8Fa5q9ugsRl0V-R3aukUXDT2aISxD1LnIdS6cdqh_MFpt1p9xURTR-_Jtfk0uKtN4vBnnjHw8LzeLVbx-fSkW83VsQdIhlowZanjKwIBKw5JSUWUIHKU1W06ZsYIr3JYgVBghAxIFLxlXtKKKw4w8HHt3ffe9Rz_otvYWm8Y47PZeZ1kOmUj_B5lQPKNKBDA5grbvvO-x0ru-bk3_qxnVB686eNUHrxp08BoCd2PzfttiecJHkeF_P_6Nt6apeuNs7U9YJlPBeAZ_Rmp9Pg</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>MCBRIDE, J. 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B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-511a0a2613a386a26604f7e32e5cab201ac428ebd3488eb35035e42d1280f0823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>AIDS/HIV</topic><topic>Antibodies, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Blood Specimen Collection - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV - immunology</topic><topic>HIV Antibodies</topic><topic>human immunodeficiency virus</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Microbiology</topic><topic>Predictive Value of Tests</topic><topic>Reagent Kits, Diagnostic</topic><topic>Techniques used in virology</topic><topic>Temperature</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MCBRIDE, J. H</creatorcontrib><creatorcontrib>HOWANITZ, P. J</creatorcontrib><creatorcontrib>RODGERSON, D. O</creatorcontrib><creatorcontrib>MILES, J</creatorcontrib><creatorcontrib>PETER, J. B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS research and human retroviruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MCBRIDE, J. H</au><au>HOWANITZ, P. J</au><au>RODGERSON, D. O</au><au>MILES, J</au><au>PETER, J. B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of specimen treatment on nonreactive HTLV-III SERA</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>1987</date><risdate>1987</risdate><volume>3</volume><issue>3</issue><spage>333</spage><epage>340</epage><pages>333-340</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>We investigated effects of specimen treatment on HTLV-III antibody assay results. Freezing and thawing specimens 10 times did not increase specimen mean absorbance (A) values measured by the Abbott immunoenzymometric assay (IEMA). Heating blood donor serum specimens at 56 degrees C for at least 10 min inactivates virus, but converted 7.1% of nonreactive specimens to positive when measured by this IEMA. Also heating at 56 degrees C for 30 min increased A values of most specimens; however, the conversion to positive within assay ranged from 0 to 75%, with a mean of 17%. When 56 nonreactive patient specimens were heated, 55% became Abbott IEMA reactive, although ENI IEMA values did not increase and Western blot results remained nonreactive. Binding of immunoglobulins G and A to Abbott IEMA beads did not cause this conversion after heat treatment. We postulate that heating specimens causes alteration of some serum matrices and introduces nonspecific binding to assay bead antigen or antibody reagents. We recommend that for the Abbott IEMA, specimens may be stored frozen, and must not be heated to avoid altered reactivity.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>3325086</pmid><doi>10.1089/aid.1987.3.333</doi><tpages>8</tpages></addata></record> |
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| ispartof | AIDS research and human retroviruses, 1987, Vol.3 (3), p.333-340 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | AIDS/HIV Antibodies, Viral - analysis Biological and medical sciences Blood Specimen Collection - methods Fundamental and applied biological sciences. Psychology HIV - immunology HIV Antibodies human immunodeficiency virus Humans Immunoenzyme Techniques Microbiology Predictive Value of Tests Reagent Kits, Diagnostic Techniques used in virology Temperature Virology |
| title | Influence of specimen treatment on nonreactive HTLV-III SERA |
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