Crystallographic studies and preliminary X-ray investigation of (S)-p-hydroxy-mandelonitrile lyase from Sorghum bicolor (L.)

(S)‐p‐Hydroxy‐mandelonitrile lyase from Sorghum bicolor has been crystallized in three different forms using the hanging‐drop vapor‐diffusion technique. Crystal form I is obtained from 1.4 M (NH4)2SO4 in 100 mM Na‐acetate, pH 4.6, and belongs to the orthorhombic space group P212121. The cell dimensions are a = 71.4, b = 95.8, c = 149.1 Å. A complete set of diffraction data has been collected to 2.6 Å resolution. Form II crystals are grown from 500 mM Li2SO4 in 13% polyethylene glycol 8000. These crystals appear as hexagonal plates and diffract to 2.98 Å resolution but apparently are twinned. Cocrystallizing hydroxynitrile lyase with the inhibitor benzoic acid using 1.4 M (NH4)2SO4 in 100 mM Na citrate, pH 5.4 as precipitant yields crystal form III, which belongs to the monoclinic space group C2 with a = 150.7, b = 103.7, c = 90.6 Å, β = 101.3. X‐ray diffraction data were collected to 2.3 Å resolution.