Discriminating between Ixodes Ticks by Means of Mitochondrial DNA Sequences
Ticks of the genus Ixodes have recently assumed prominence because they frequently serve as vectors of important zoonoses, including Lyme disease and babesiosis. The morphological characteristics that have been used in their identification often are ambiguous and are useful solely at a particular st...
Gespeichert in:
| Veröffentlicht in: | Molecular phylogenetics and evolution 1995-12, Vol.4 (4), p.361-365 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 365 |
|---|---|
| container_issue | 4 |
| container_start_page | 361 |
| container_title | Molecular phylogenetics and evolution |
| container_volume | 4 |
| creator | Caporale, Diane A. Rich, Stephen M. Spielman, Andrew Telford, Sam R. Kocher, Thomas D. |
| description | Ticks of the genus
Ixodes have recently assumed prominence because they frequently serve as vectors of important zoonoses, including Lyme disease and babesiosis. The morphological characteristics that have been used in their identification often are ambiguous and are useful solely at a particular stage of development. Here we report the DNA sequence of the mitochondrially encoded 16S rRNA gene of nine different
Ixodes ticks and an outgroup from another genus,
Dermacentor. The sequences readily discriminate between these ticks. Samples of
I. dammini from the northeastern and upper midwestern United States differ from southeastern
I. scapularis at about 2% of the nucleotides. This difference is about half that separating other members of the
I. ricinus group of species, but exceeds typical levels of intraspecific variation. Two major clades exist within the
I. ricinus complex. One includes
I. cookei , I. hexagonus,and
I. angustus. The other includes
I. persulcatus, I. pacificus, I. muris, I. ricinus, I. scapularis, and
I. dammini. We conclude that mtDNA sequences are useful for unravelling the systematics of these important vectors of human disease. |
| doi_str_mv | 10.1006/mpev.1995.1033 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77912780</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1055790385710330</els_id><sourcerecordid>77912780</sourcerecordid><originalsourceid>FETCH-LOGICAL-c339t-415cc0d36ab932d712744634830facca428856f3351aaa65b71b05fd41c454113</originalsourceid><addsrcrecordid>eNp1kL1PwzAQxS0EKqWwsiF5YkuxYzuJx6rlo6KFgTJbjnMBQxKXOC30v8dRKzamu9O9e6f3Q-iSkjElJLmp17AdUylFGBk7QkNKpIikoOy474WIUknYKTrz_oMQSoUUAzTIUp7GMh6ix5n1prW1bXRnmzecQ_cN0OD5jyvA45U1nx7nO7wE3XjsSry0nTPvrilaqys8e5rgF_jaQGPAn6OTUlceLg51hF7vblfTh2jxfD-fThaRYUx2EafCGFKwROeSxUVK45TzhPGMkVIbo3mcZSIpGRNUa52IPKU5EWXBqeGCU8pG6Hrvu25deO07VYcQUFW6AbfxKk1l8MxIEI73QtM671so1TpE1e1OUaJ6eqqnp3p6qqcXDq4Ozpu8huJPfsAV9tl-DyHe1kKrvLF99sK2YDpVOPuf9S_3Ln1I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77912780</pqid></control><display><type>article</type><title>Discriminating between Ixodes Ticks by Means of Mitochondrial DNA Sequences</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Caporale, Diane A. ; Rich, Stephen M. ; Spielman, Andrew ; Telford, Sam R. ; Kocher, Thomas D.</creator><creatorcontrib>Caporale, Diane A. ; Rich, Stephen M. ; Spielman, Andrew ; Telford, Sam R. ; Kocher, Thomas D.</creatorcontrib><description>Ticks of the genus
Ixodes have recently assumed prominence because they frequently serve as vectors of important zoonoses, including Lyme disease and babesiosis. The morphological characteristics that have been used in their identification often are ambiguous and are useful solely at a particular stage of development. Here we report the DNA sequence of the mitochondrially encoded 16S rRNA gene of nine different
Ixodes ticks and an outgroup from another genus,
Dermacentor. The sequences readily discriminate between these ticks. Samples of
I. dammini from the northeastern and upper midwestern United States differ from southeastern
I. scapularis at about 2% of the nucleotides. This difference is about half that separating other members of the
I. ricinus group of species, but exceeds typical levels of intraspecific variation. Two major clades exist within the
I. ricinus complex. One includes
I. cookei , I. hexagonus,and
I. angustus. The other includes
I. persulcatus, I. pacificus, I. muris, I. ricinus, I. scapularis, and
I. dammini. We conclude that mtDNA sequences are useful for unravelling the systematics of these important vectors of human disease.</description><identifier>ISSN: 1055-7903</identifier><identifier>EISSN: 1095-9513</identifier><identifier>DOI: 10.1006/mpev.1995.1033</identifier><identifier>PMID: 8747292</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Arachnid Vectors ; Base Sequence ; Dermacentor - genetics ; DNA Primers - genetics ; DNA, Mitochondrial - genetics ; Genes ; Humans ; Ixodes - classification ; Ixodes - genetics ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S - genetics ; Species Specificity</subject><ispartof>Molecular phylogenetics and evolution, 1995-12, Vol.4 (4), p.361-365</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-415cc0d36ab932d712744634830facca428856f3351aaa65b71b05fd41c454113</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mpev.1995.1033$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8747292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caporale, Diane A.</creatorcontrib><creatorcontrib>Rich, Stephen M.</creatorcontrib><creatorcontrib>Spielman, Andrew</creatorcontrib><creatorcontrib>Telford, Sam R.</creatorcontrib><creatorcontrib>Kocher, Thomas D.</creatorcontrib><title>Discriminating between Ixodes Ticks by Means of Mitochondrial DNA Sequences</title><title>Molecular phylogenetics and evolution</title><addtitle>Mol Phylogenet Evol</addtitle><description>Ticks of the genus
Ixodes have recently assumed prominence because they frequently serve as vectors of important zoonoses, including Lyme disease and babesiosis. The morphological characteristics that have been used in their identification often are ambiguous and are useful solely at a particular stage of development. Here we report the DNA sequence of the mitochondrially encoded 16S rRNA gene of nine different
Ixodes ticks and an outgroup from another genus,
Dermacentor. The sequences readily discriminate between these ticks. Samples of
I. dammini from the northeastern and upper midwestern United States differ from southeastern
I. scapularis at about 2% of the nucleotides. This difference is about half that separating other members of the
I. ricinus group of species, but exceeds typical levels of intraspecific variation. Two major clades exist within the
I. ricinus complex. One includes
I. cookei , I. hexagonus,and
I. angustus. The other includes
I. persulcatus, I. pacificus, I. muris, I. ricinus, I. scapularis, and
I. dammini. We conclude that mtDNA sequences are useful for unravelling the systematics of these important vectors of human disease.</description><subject>Animals</subject><subject>Arachnid Vectors</subject><subject>Base Sequence</subject><subject>Dermacentor - genetics</subject><subject>DNA Primers - genetics</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Genes</subject><subject>Humans</subject><subject>Ixodes - classification</subject><subject>Ixodes - genetics</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Species Specificity</subject><issn>1055-7903</issn><issn>1095-9513</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL1PwzAQxS0EKqWwsiF5YkuxYzuJx6rlo6KFgTJbjnMBQxKXOC30v8dRKzamu9O9e6f3Q-iSkjElJLmp17AdUylFGBk7QkNKpIikoOy474WIUknYKTrz_oMQSoUUAzTIUp7GMh6ix5n1prW1bXRnmzecQ_cN0OD5jyvA45U1nx7nO7wE3XjsSry0nTPvrilaqys8e5rgF_jaQGPAn6OTUlceLg51hF7vblfTh2jxfD-fThaRYUx2EafCGFKwROeSxUVK45TzhPGMkVIbo3mcZSIpGRNUa52IPKU5EWXBqeGCU8pG6Hrvu25deO07VYcQUFW6AbfxKk1l8MxIEI73QtM671so1TpE1e1OUaJ6eqqnp3p6qqcXDq4Ozpu8huJPfsAV9tl-DyHe1kKrvLF99sK2YDpVOPuf9S_3Ln1I</recordid><startdate>19951201</startdate><enddate>19951201</enddate><creator>Caporale, Diane A.</creator><creator>Rich, Stephen M.</creator><creator>Spielman, Andrew</creator><creator>Telford, Sam R.</creator><creator>Kocher, Thomas D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951201</creationdate><title>Discriminating between Ixodes Ticks by Means of Mitochondrial DNA Sequences</title><author>Caporale, Diane A. ; Rich, Stephen M. ; Spielman, Andrew ; Telford, Sam R. ; Kocher, Thomas D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-415cc0d36ab932d712744634830facca428856f3351aaa65b71b05fd41c454113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Arachnid Vectors</topic><topic>Base Sequence</topic><topic>Dermacentor - genetics</topic><topic>DNA Primers - genetics</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Genes</topic><topic>Humans</topic><topic>Ixodes - classification</topic><topic>Ixodes - genetics</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caporale, Diane A.</creatorcontrib><creatorcontrib>Rich, Stephen M.</creatorcontrib><creatorcontrib>Spielman, Andrew</creatorcontrib><creatorcontrib>Telford, Sam R.</creatorcontrib><creatorcontrib>Kocher, Thomas D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular phylogenetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caporale, Diane A.</au><au>Rich, Stephen M.</au><au>Spielman, Andrew</au><au>Telford, Sam R.</au><au>Kocher, Thomas D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discriminating between Ixodes Ticks by Means of Mitochondrial DNA Sequences</atitle><jtitle>Molecular phylogenetics and evolution</jtitle><addtitle>Mol Phylogenet Evol</addtitle><date>1995-12-01</date><risdate>1995</risdate><volume>4</volume><issue>4</issue><spage>361</spage><epage>365</epage><pages>361-365</pages><issn>1055-7903</issn><eissn>1095-9513</eissn><abstract>Ticks of the genus
Ixodes have recently assumed prominence because they frequently serve as vectors of important zoonoses, including Lyme disease and babesiosis. The morphological characteristics that have been used in their identification often are ambiguous and are useful solely at a particular stage of development. Here we report the DNA sequence of the mitochondrially encoded 16S rRNA gene of nine different
Ixodes ticks and an outgroup from another genus,
Dermacentor. The sequences readily discriminate between these ticks. Samples of
I. dammini from the northeastern and upper midwestern United States differ from southeastern
I. scapularis at about 2% of the nucleotides. This difference is about half that separating other members of the
I. ricinus group of species, but exceeds typical levels of intraspecific variation. Two major clades exist within the
I. ricinus complex. One includes
I. cookei , I. hexagonus,and
I. angustus. The other includes
I. persulcatus, I. pacificus, I. muris, I. ricinus, I. scapularis, and
I. dammini. We conclude that mtDNA sequences are useful for unravelling the systematics of these important vectors of human disease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8747292</pmid><doi>10.1006/mpev.1995.1033</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1055-7903 |
| ispartof | Molecular phylogenetics and evolution, 1995-12, Vol.4 (4), p.361-365 |
| issn | 1055-7903 1095-9513 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77912780 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Arachnid Vectors Base Sequence Dermacentor - genetics DNA Primers - genetics DNA, Mitochondrial - genetics Genes Humans Ixodes - classification Ixodes - genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S - genetics Species Specificity |
| title | Discriminating between Ixodes Ticks by Means of Mitochondrial DNA Sequences |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T12%3A18%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Discriminating%20between%20Ixodes%20Ticks%20by%20Means%20of%20Mitochondrial%20DNA%20Sequences&rft.jtitle=Molecular%20phylogenetics%20and%20evolution&rft.au=Caporale,%20Diane%20A.&rft.date=1995-12-01&rft.volume=4&rft.issue=4&rft.spage=361&rft.epage=365&rft.pages=361-365&rft.issn=1055-7903&rft.eissn=1095-9513&rft_id=info:doi/10.1006/mpev.1995.1033&rft_dat=%3Cproquest_cross%3E77912780%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77912780&rft_id=info:pmid/8747292&rft_els_id=S1055790385710330&rfr_iscdi=true |