Monitoring of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma Microsamples Using High-Performance Liquid Chromatography with Coulometric Electrochemical Detection
A method for measuring the lipophilic antioxidants α-tocopherol, γ-tocopherol, ubiquinol-10, ubiquinone-10, β-cryptoxanthin, and β-carotene in 5 or 10 μl neonatal plasma is presented. First lipid extraction together with the internal standards γ-tocotrienol, ubiquinone-7, ubiquinol-9, ubiquinone-9,...
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| Veröffentlicht in: | Analytical biochemistry 1995-12, Vol.232 (2), p.210-216 |
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| container_title | Analytical biochemistry |
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| creator | Finckh, Barbara Kontush, Anatol Commentz, Jens Hübner, Christoph Burdelski, Martin Kohlschütter, Alfried |
| description | A method for measuring the lipophilic antioxidants α-tocopherol, γ-tocopherol, ubiquinol-10, ubiquinone-10, β-cryptoxanthin, and β-carotene in 5 or 10 μl neonatal plasma is presented. First lipid extraction together with the internal standards γ-tocotrienol, ubiquinone-7, ubiquinol-9, ubiquinone-9, and ethyl β-apo-8′-carotenoate was carried out. Then the components were separated on a RPC2/C18column. Coulometric electrochemical detection was performed using the reduction–oxidation mode (−0.60 V; −0.15 V; +0.60 V). Detection limits for all components were between 21 and 60 fmol, corresponding to 0.004 and 0.012 μmol/liter plasma; the within-day precision (coefficient of variation) was between 3 and 14% for all measured substances. Reference values are given for healthy newborn children from the second to the sixth day of life. Using the presented HPLC technique it is possible to monitor the concentrations of lipophilic antioxidants in plasma of sick immature newborn children in order to study effects of oxidative stress. |
| doi_str_mv | 10.1006/abio.1995.0009 |
| format | Article |
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First lipid extraction together with the internal standards γ-tocotrienol, ubiquinone-7, ubiquinol-9, ubiquinone-9, and ethyl β-apo-8′-carotenoate was carried out. Then the components were separated on a RPC2/C18column. Coulometric electrochemical detection was performed using the reduction–oxidation mode (−0.60 V; −0.15 V; +0.60 V). Detection limits for all components were between 21 and 60 fmol, corresponding to 0.004 and 0.012 μmol/liter plasma; the within-day precision (coefficient of variation) was between 3 and 14% for all measured substances. Reference values are given for healthy newborn children from the second to the sixth day of life. Using the presented HPLC technique it is possible to monitor the concentrations of lipophilic antioxidants in plasma of sick immature newborn children in order to study effects of oxidative stress.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.1995.0009</identifier><identifier>PMID: 8747477</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antioxidants - analysis ; beta Carotene ; Carotenoids - analogs & derivatives ; Carotenoids - blood ; Chromatography, High Pressure Liquid - methods ; Cryptoxanthins ; Electrochemistry ; Humans ; Infant, Newborn - blood ; Microchemistry ; Oxidation-Reduction ; Oxidative Stress ; Reference Standards ; Reference Values ; Ubiquinone - analogs & derivatives ; Ubiquinone - blood ; Vitamin E - blood ; Xanthophylls</subject><ispartof>Analytical biochemistry, 1995-12, Vol.232 (2), p.210-216</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-af5872b3628e3049e10224b1c3fd00470a45d4268870faba27dbce1fb4d419023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abio.1995.0009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8747477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Finckh, Barbara</creatorcontrib><creatorcontrib>Kontush, Anatol</creatorcontrib><creatorcontrib>Commentz, Jens</creatorcontrib><creatorcontrib>Hübner, Christoph</creatorcontrib><creatorcontrib>Burdelski, Martin</creatorcontrib><creatorcontrib>Kohlschütter, Alfried</creatorcontrib><title>Monitoring of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma Microsamples Using High-Performance Liquid Chromatography with Coulometric Electrochemical Detection</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A method for measuring the lipophilic antioxidants α-tocopherol, γ-tocopherol, ubiquinol-10, ubiquinone-10, β-cryptoxanthin, and β-carotene in 5 or 10 μl neonatal plasma is presented. First lipid extraction together with the internal standards γ-tocotrienol, ubiquinone-7, ubiquinol-9, ubiquinone-9, and ethyl β-apo-8′-carotenoate was carried out. Then the components were separated on a RPC2/C18column. Coulometric electrochemical detection was performed using the reduction–oxidation mode (−0.60 V; −0.15 V; +0.60 V). Detection limits for all components were between 21 and 60 fmol, corresponding to 0.004 and 0.012 μmol/liter plasma; the within-day precision (coefficient of variation) was between 3 and 14% for all measured substances. Reference values are given for healthy newborn children from the second to the sixth day of life. Using the presented HPLC technique it is possible to monitor the concentrations of lipophilic antioxidants in plasma of sick immature newborn children in order to study effects of oxidative stress.</description><subject>Antioxidants - analysis</subject><subject>beta Carotene</subject><subject>Carotenoids - analogs & derivatives</subject><subject>Carotenoids - blood</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cryptoxanthins</subject><subject>Electrochemistry</subject><subject>Humans</subject><subject>Infant, Newborn - blood</subject><subject>Microchemistry</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress</subject><subject>Reference Standards</subject><subject>Reference Values</subject><subject>Ubiquinone - analogs & derivatives</subject><subject>Ubiquinone - blood</subject><subject>Vitamin E - blood</subject><subject>Xanthophylls</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcFu1DAQjRCoLIUrNySfODXLOMkm8RGlhSJtoYfu2XLsycbI8aR2AurX8Wsk7Ko3NIfRzLx5TzMvSd5z2HKA8pNqLW25ELstAIgXyYaDKFPIQbxMNksrT7NSVK-TNzH-BOC82JUXyUVdFUtUm-TPHXk7UbD-yKhjh9Y-ztaTSzlcPVce_5WNCjShJ2viFVPesAfSNPYYyEVmPfuO5NWkHLt3Kg6K3VkdKKphdBjZIa4St_bYp_cYOgqD8hrZflUwrOkDDWqiY1Bj_8R-26lnDc2OBpyC1ezGoZ4C6R4HqxeFa5yWhiX_NnnVKRfx3TlfJocvNw_Nbbr_8fVb83mf6jwXU6q6XV1lbV5mNeZQCOSQZUXLdd4ZgKICVexMkZV1XUGnWpVVptXIu7YwBReQ5ZfJxxPvGOhxxjjJwUaNzimPNEdZVQIqzmEBbk_A9fYYsJNjsIMKT5KDXB2Tq2NydUyuji0LH87MczugeYafLVrm9WmOy3m_LAYZtcXld8aG5QfSkP0f9V99jKir</recordid><startdate>19951210</startdate><enddate>19951210</enddate><creator>Finckh, Barbara</creator><creator>Kontush, Anatol</creator><creator>Commentz, Jens</creator><creator>Hübner, Christoph</creator><creator>Burdelski, Martin</creator><creator>Kohlschütter, Alfried</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951210</creationdate><title>Monitoring of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma Microsamples Using High-Performance Liquid Chromatography with Coulometric Electrochemical Detection</title><author>Finckh, Barbara ; Kontush, Anatol ; Commentz, Jens ; Hübner, Christoph ; Burdelski, Martin ; Kohlschütter, Alfried</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-af5872b3628e3049e10224b1c3fd00470a45d4268870faba27dbce1fb4d419023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Antioxidants - analysis</topic><topic>beta Carotene</topic><topic>Carotenoids - analogs & derivatives</topic><topic>Carotenoids - blood</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cryptoxanthins</topic><topic>Electrochemistry</topic><topic>Humans</topic><topic>Infant, Newborn - blood</topic><topic>Microchemistry</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress</topic><topic>Reference Standards</topic><topic>Reference Values</topic><topic>Ubiquinone - analogs & derivatives</topic><topic>Ubiquinone - blood</topic><topic>Vitamin E - blood</topic><topic>Xanthophylls</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finckh, Barbara</creatorcontrib><creatorcontrib>Kontush, Anatol</creatorcontrib><creatorcontrib>Commentz, Jens</creatorcontrib><creatorcontrib>Hübner, Christoph</creatorcontrib><creatorcontrib>Burdelski, Martin</creatorcontrib><creatorcontrib>Kohlschütter, Alfried</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finckh, Barbara</au><au>Kontush, Anatol</au><au>Commentz, Jens</au><au>Hübner, Christoph</au><au>Burdelski, Martin</au><au>Kohlschütter, Alfried</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma Microsamples Using High-Performance Liquid Chromatography with Coulometric Electrochemical Detection</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1995-12-10</date><risdate>1995</risdate><volume>232</volume><issue>2</issue><spage>210</spage><epage>216</epage><pages>210-216</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A method for measuring the lipophilic antioxidants α-tocopherol, γ-tocopherol, ubiquinol-10, ubiquinone-10, β-cryptoxanthin, and β-carotene in 5 or 10 μl neonatal plasma is presented. First lipid extraction together with the internal standards γ-tocotrienol, ubiquinone-7, ubiquinol-9, ubiquinone-9, and ethyl β-apo-8′-carotenoate was carried out. Then the components were separated on a RPC2/C18column. Coulometric electrochemical detection was performed using the reduction–oxidation mode (−0.60 V; −0.15 V; +0.60 V). Detection limits for all components were between 21 and 60 fmol, corresponding to 0.004 and 0.012 μmol/liter plasma; the within-day precision (coefficient of variation) was between 3 and 14% for all measured substances. Reference values are given for healthy newborn children from the second to the sixth day of life. Using the presented HPLC technique it is possible to monitor the concentrations of lipophilic antioxidants in plasma of sick immature newborn children in order to study effects of oxidative stress.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8747477</pmid><doi>10.1006/abio.1995.0009</doi><tpages>7</tpages></addata></record> |
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| subjects | Antioxidants - analysis beta Carotene Carotenoids - analogs & derivatives Carotenoids - blood Chromatography, High Pressure Liquid - methods Cryptoxanthins Electrochemistry Humans Infant, Newborn - blood Microchemistry Oxidation-Reduction Oxidative Stress Reference Standards Reference Values Ubiquinone - analogs & derivatives Ubiquinone - blood Vitamin E - blood Xanthophylls |
| title | Monitoring of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma Microsamples Using High-Performance Liquid Chromatography with Coulometric Electrochemical Detection |
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