Characterization of the Catalase-Peroxidase Gene (katG) and inhA Locus in Isoniazid-Resistant and -Susceptible Strains of Mycobacterium tuberculosis by Automated DNA Sequencing: Restricted Array of Mutations Associated with Drug Resistance
The catalase-peroxidase gene (katG) and a two-genelocus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12 susceptible strains. Virtually all resistant organisms had amino acid changes in KatG or nucleotide substitu...
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| Veröffentlicht in: | The Journal of infectious diseases 1996-01, Vol.173 (1), p.196-202 |
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| container_title | The Journal of infectious diseases |
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| creator | Musser, James M. Kapur, Vivek Williams, Diana L. Kreiswirth, Barry N. van Soolingen, Dick van Embden, Jan D. A. |
| description | The catalase-peroxidase gene (katG) and a two-genelocus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12 susceptible strains. Virtually all resistant organisms had amino acid changes in KatG or nucleotide substitutions upstream of inhA. A region of katG encoding two amino acids frequently altered in resistant strains (residues Ser31S and Arg463) and the inhA locus were sequenced in 10 susceptible and 51 isoniazid-resistant isolates from the Netherlands. Most (84%) of the resistant isolates had mutations in katG or the inhA locus or lacked katG. Together, ∼75% of isoniazid-resistant isolates had replacements at amino acids 315 or 463 in KatG or nucleotide substitutions upstream of inhA. All 16 strains of Mycobacterium bovis and Mycobacterium microti studied had Leu463 rather than Arg463in KatG, an observation consistent with the hypothesis that Leu463is the ancestral condition in M. tuberculosis. |
| doi_str_mv | 10.1093/infdis/173.1.196 |
| format | Article |
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A.</creator><creatorcontrib>Musser, James M. ; Kapur, Vivek ; Williams, Diana L. ; Kreiswirth, Barry N. ; van Soolingen, Dick ; van Embden, Jan D. A.</creatorcontrib><description>The catalase-peroxidase gene (katG) and a two-genelocus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12 susceptible strains. Virtually all resistant organisms had amino acid changes in KatG or nucleotide substitutions upstream of inhA. A region of katG encoding two amino acids frequently altered in resistant strains (residues Ser31S and Arg463) and the inhA locus were sequenced in 10 susceptible and 51 isoniazid-resistant isolates from the Netherlands. Most (84%) of the resistant isolates had mutations in katG or the inhA locus or lacked katG. Together, ∼75% of isoniazid-resistant isolates had replacements at amino acids 315 or 463 in KatG or nucleotide substitutions upstream of inhA. All 16 strains of Mycobacterium bovis and Mycobacterium microti studied had Leu463 rather than Arg463in KatG, an observation consistent with the hypothesis that Leu463is the ancestral condition in M. tuberculosis.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/173.1.196</identifier><identifier>PMID: 8537659</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Amino Acid Sequence ; Amino acids ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antitubercular Agents - pharmacology ; Bacterial Proteins - genetics ; Base Sequence ; Biological and medical sciences ; Catalase - genetics ; Codons ; DNA ; DNA, Bacterial - analysis ; Drug Resistance, Microbial - genetics ; Genes, Bacterial ; Genetic loci ; Genetic mutation ; Genetic variation ; Humans ; Isoniazid - pharmacology ; Medical sciences ; Molecular Sequence Data ; Mutation ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - enzymology ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Nucleotides ; Oxidoreductases ; Peroxidase - genetics ; Peroxidases - genetics ; Pharmacology. Drug treatments ; Sequence Analysis, DNA - methods ; Sequencing ; Tuberculosis ; Tuberculosis - microbiology</subject><ispartof>The Journal of infectious diseases, 1996-01, Vol.173 (1), p.196-202</ispartof><rights>Copyright 1996 The University of Chicago</rights><rights>1996 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Jan 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-ff5bab6622a1cccf1a2417323858bb4f80f6c9717c6cbb444738ebbfd64a4ef73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30127963$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30127963$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2997203$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8537659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Musser, James M.</creatorcontrib><creatorcontrib>Kapur, Vivek</creatorcontrib><creatorcontrib>Williams, Diana L.</creatorcontrib><creatorcontrib>Kreiswirth, Barry N.</creatorcontrib><creatorcontrib>van Soolingen, Dick</creatorcontrib><creatorcontrib>van Embden, Jan D. A.</creatorcontrib><title>Characterization of the Catalase-Peroxidase Gene (katG) and inhA Locus in Isoniazid-Resistant and -Susceptible Strains of Mycobacterium tuberculosis by Automated DNA Sequencing: Restricted Array of Mutations Associated with Drug Resistance</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>The catalase-peroxidase gene (katG) and a two-genelocus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12 susceptible strains. Virtually all resistant organisms had amino acid changes in KatG or nucleotide substitutions upstream of inhA. A region of katG encoding two amino acids frequently altered in resistant strains (residues Ser31S and Arg463) and the inhA locus were sequenced in 10 susceptible and 51 isoniazid-resistant isolates from the Netherlands. Most (84%) of the resistant isolates had mutations in katG or the inhA locus or lacked katG. Together, ∼75% of isoniazid-resistant isolates had replacements at amino acids 315 or 463 in KatG or nucleotide substitutions upstream of inhA. All 16 strains of Mycobacterium bovis and Mycobacterium microti studied had Leu463 rather than Arg463in KatG, an observation consistent with the hypothesis that Leu463is the ancestral condition in M. tuberculosis.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antitubercular Agents - pharmacology</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Catalase - genetics</subject><subject>Codons</subject><subject>DNA</subject><subject>DNA, Bacterial - analysis</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Genes, Bacterial</subject><subject>Genetic loci</subject><subject>Genetic mutation</subject><subject>Genetic variation</subject><subject>Humans</subject><subject>Isoniazid - pharmacology</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - enzymology</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Nucleotides</subject><subject>Oxidoreductases</subject><subject>Peroxidase - genetics</subject><subject>Peroxidases - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Sequence Analysis, DNA - methods</subject><subject>Sequencing</subject><subject>Tuberculosis</subject><subject>Tuberculosis - microbiology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUklvEzEUHiFQCYU7FyQLIQSHSb1M7JneRimklcKiJkiIi-XxeBqnEzt4EU3_NH8BZ2mQuHDys77lPT9_WfYSwSGCFTnTpmu1P0OMDNEQVfRRNkAjwnJKEXmcDSDEOEdlVT3Nnnm_hBAWhLKT7KRMJDqqBtnv8UI4IYNy-l4EbQ2wHQgLBcYiiF54lX9Vzt7pNpVgoowC725FmLwHwrRAm0UNplZGn0pw5a3R4l63-bXy2gdhwo6Vz6KXah100yswC05o47ddPm2kbfat4wqE2CgnY2-TFDQbUMdgVyKoFlx8rsFM_YzKSG1uzkFyD07LLVQ7JzY7rxh203tQe2-l3gl_6bAAFy7egIeBpHqePelE79WLw3maffv4YT6-zKdfJlfjeprLoiQh77pRIxpKMRZIStkhgYu0ZEzKUdk0RVfCjsqKISapTPeiYKRUTdO1tBCF6hg5zd7ufdfOptF94CudttD3wigbPWesrEqM6H-JiMERqooiEV__Q1za6Ex6BMeYVIhCjBIJ7knSWe-d6vja6ZVwG44g3yaG7xOTbAlHPCUmSV4dfGOzUu1RcIhIwt8ccOGl6DuX1pgMHmi4qhiG5K_N0gfrjjCBCLPUJeH5Hk8foe6OuHC3nDLCRvzy-w8-R3M2vcaMY_IHXq_oLQ</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Musser, James M.</creator><creator>Kapur, Vivek</creator><creator>Williams, Diana L.</creator><creator>Kreiswirth, Barry N.</creator><creator>van Soolingen, Dick</creator><creator>van Embden, Jan D. A.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Characterization of the Catalase-Peroxidase Gene (katG) and inhA Locus in Isoniazid-Resistant and -Susceptible Strains of Mycobacterium tuberculosis by Automated DNA Sequencing: Restricted Array of Mutations Associated with Drug Resistance</title><author>Musser, James M. ; Kapur, Vivek ; Williams, Diana L. ; Kreiswirth, Barry N. ; van Soolingen, Dick ; van Embden, Jan D. 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Antiparasitic agents</topic><topic>Antitubercular Agents - pharmacology</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Catalase - genetics</topic><topic>Codons</topic><topic>DNA</topic><topic>DNA, Bacterial - analysis</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Genes, Bacterial</topic><topic>Genetic loci</topic><topic>Genetic mutation</topic><topic>Genetic variation</topic><topic>Humans</topic><topic>Isoniazid - pharmacology</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Mycobacterium tuberculosis - enzymology</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Nucleotides</topic><topic>Oxidoreductases</topic><topic>Peroxidase - genetics</topic><topic>Peroxidases - genetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Sequence Analysis, DNA - methods</topic><topic>Sequencing</topic><topic>Tuberculosis</topic><topic>Tuberculosis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Musser, James M.</creatorcontrib><creatorcontrib>Kapur, Vivek</creatorcontrib><creatorcontrib>Williams, Diana L.</creatorcontrib><creatorcontrib>Kreiswirth, Barry N.</creatorcontrib><creatorcontrib>van Soolingen, Dick</creatorcontrib><creatorcontrib>van Embden, Jan D. 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A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the Catalase-Peroxidase Gene (katG) and inhA Locus in Isoniazid-Resistant and -Susceptible Strains of Mycobacterium tuberculosis by Automated DNA Sequencing: Restricted Array of Mutations Associated with Drug Resistance</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1996-01</date><risdate>1996</risdate><volume>173</volume><issue>1</issue><spage>196</spage><epage>202</epage><pages>196-202</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The catalase-peroxidase gene (katG) and a two-genelocus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12 susceptible strains. Virtually all resistant organisms had amino acid changes in KatG or nucleotide substitutions upstream of inhA. A region of katG encoding two amino acids frequently altered in resistant strains (residues Ser31S and Arg463) and the inhA locus were sequenced in 10 susceptible and 51 isoniazid-resistant isolates from the Netherlands. Most (84%) of the resistant isolates had mutations in katG or the inhA locus or lacked katG. Together, ∼75% of isoniazid-resistant isolates had replacements at amino acids 315 or 463 in KatG or nucleotide substitutions upstream of inhA. All 16 strains of Mycobacterium bovis and Mycobacterium microti studied had Leu463 rather than Arg463in KatG, an observation consistent with the hypothesis that Leu463is the ancestral condition in M. tuberculosis.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>8537659</pmid><doi>10.1093/infdis/173.1.196</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | The Journal of infectious diseases, 1996-01, Vol.173 (1), p.196-202 |
| issn | 0022-1899 1537-6613 |
| language | eng |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Amino Acid Sequence Amino acids Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Antitubercular Agents - pharmacology Bacterial Proteins - genetics Base Sequence Biological and medical sciences Catalase - genetics Codons DNA DNA, Bacterial - analysis Drug Resistance, Microbial - genetics Genes, Bacterial Genetic loci Genetic mutation Genetic variation Humans Isoniazid - pharmacology Medical sciences Molecular Sequence Data Mutation Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - enzymology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Nucleotides Oxidoreductases Peroxidase - genetics Peroxidases - genetics Pharmacology. Drug treatments Sequence Analysis, DNA - methods Sequencing Tuberculosis Tuberculosis - microbiology |
| title | Characterization of the Catalase-Peroxidase Gene (katG) and inhA Locus in Isoniazid-Resistant and -Susceptible Strains of Mycobacterium tuberculosis by Automated DNA Sequencing: Restricted Array of Mutations Associated with Drug Resistance |
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