Characterization of the ksgA gene of Escherichia coli determining kasugamycin sensitivity

In the plasmid pUC8ksgA7 [1], the codind region of the ksgA gene is preceded by the lac promoter (Plac) and a small open reading frame (ORF). This ORF of 15 codons is composed of nucleotides derived from the lacZ gene, a multiple cloning site and the ksgA gene itself. The reading frame begins with t...

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Veröffentlicht in:	Biochimie 1987-08, Vol.69 (8), p.841-848
Hauptverfasser:	Van Gemen, B, Koets, H.J, Plooy, C.A.M, Bodlaender, J, Van Knippenberg, P.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoglycosides Anti-Bacterial Agents - pharmacology Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular Drug Resistance, Microbial Escherichia coli Escherichia coli - drug effects Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Methods. Procedures. Technologies Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Promoter Regions, Genetic Translation. Translation factors. Protein processing Vectors (cloning, transfer, expression). Insertion sequences and transposons
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