Mechanical slicing of frozen brain tissue: a reappraisal of catecholamine loss
Catecholamine levels in mechanically sliced frozen bovine brain tissue were compared with those found in hand-cut, unfrozen tissue. Hemispheric content of catecholamines in small regions of cortex, caudate, nucleus accumbens, thalamus, and hippocampus in bovine brain was no different from that seen...
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| Veröffentlicht in: | Journal of neuroscience methods 1987-11, Vol.22 (1), p.41-46 |
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| container_start_page | 41 |
| container_title | Journal of neuroscience methods |
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| creator | Oke, A.F. Moghaddam, M. Ayetey, W.E.A. Adams, R.N. |
| description | Catecholamine levels in mechanically sliced frozen bovine brain tissue were compared with those found in hand-cut, unfrozen tissue. Hemispheric content of catecholamines in small regions of cortex, caudate, nucleus accumbens, thalamus, and hippocampus in bovine brain was no different from that seen in the homologous area of the contralateral hemisphere, despite selective slicing of opposing hemispheres with the two techniques. Brodmann area 25 in human brain (previously suspected to be highly vulnerable to degradative loss of catecholamines by mechanical slicing) is shown to be a restricted area of dense catecholamine concentration when compared to surrounding cortical tissue. The usage of Brodmann area 25 for technique comparisons without precise control of cortical delineation is questioned. |
| doi_str_mv | 10.1016/0165-0270(87)90087-2 |
| format | Article |
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Hemispheric content of catecholamines in small regions of cortex, caudate, nucleus accumbens, thalamus, and hippocampus in bovine brain was no different from that seen in the homologous area of the contralateral hemisphere, despite selective slicing of opposing hemispheres with the two techniques. Brodmann area 25 in human brain (previously suspected to be highly vulnerable to degradative loss of catecholamines by mechanical slicing) is shown to be a restricted area of dense catecholamine concentration when compared to surrounding cortical tissue. The usage of Brodmann area 25 for technique comparisons without precise control of cortical delineation is questioned.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(87)90087-2</identifier><identifier>PMID: 3695566</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Brain Chemistry ; Catecholamine content ; Catecholamines - analysis ; Cattle ; Frozen brain tissue ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. 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Hemispheric content of catecholamines in small regions of cortex, caudate, nucleus accumbens, thalamus, and hippocampus in bovine brain was no different from that seen in the homologous area of the contralateral hemisphere, despite selective slicing of opposing hemispheres with the two techniques. Brodmann area 25 in human brain (previously suspected to be highly vulnerable to degradative loss of catecholamines by mechanical slicing) is shown to be a restricted area of dense catecholamine concentration when compared to surrounding cortical tissue. The usage of Brodmann area 25 for technique comparisons without precise control of cortical delineation is questioned.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain Chemistry</subject><subject>Catecholamine content</subject><subject>Catecholamines - analysis</subject><subject>Cattle</subject><subject>Frozen brain tissue</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Human brain</subject><subject>Humans</subject><subject>Mechanical slicing</subject><subject>Microtomy</subject><subject>Tissue Preservation</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rFEEQhpugxDXmH0SYg4geRqtnevojh4AEvyDRi4K3pqamWltmZ9buWSH-enuzyx710BR0PW9R9QhxIeGVBKlfl9fV0Bh4Yc1LB2BN3ZyIlbSmqbWx3x6I1RF5JB7n_BMAlAN9Kk5b7bpO65X4dMv0A6dIOFZ5jBSn79UcqpDmPzxVfcI4VUvMecuXFVaJcbMpf7nQhSJcSnoecR0nrsY55yfiYcAx8_mhnomv795-uf5Q33x-__H6zU1NSpqlph6ZBiLbNqgIOudQKqMlBMOadOjZQWCpGFvpemxsTwFQt5IHY3Xft2fi-X7uJs2_tpwXv46ZeBxx4nmbvTHWQWfkf0GpbKNVqwqo9iClckfi4DcprjHdeQl-59vvZPqdTG-Nv_ftmxJ7epi_7dc8HEMHwaX_7NDHXByHhBPFfMRMJzWAK9jVHuMi7Xfk5DNFnoiHmJgWP8zx33v8BRD4nJI</recordid><startdate>19871101</startdate><enddate>19871101</enddate><creator>Oke, A.F.</creator><creator>Moghaddam, M.</creator><creator>Ayetey, W.E.A.</creator><creator>Adams, R.N.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19871101</creationdate><title>Mechanical slicing of frozen brain tissue: a reappraisal of catecholamine loss</title><author>Oke, A.F. ; Moghaddam, M. ; Ayetey, W.E.A. ; Adams, R.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-cbaecdcc832a4c0599a147610f7e6c6fbe90fe14ea319ba28bcf0a631ed786bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain Chemistry</topic><topic>Catecholamine content</topic><topic>Catecholamines - analysis</topic><topic>Cattle</topic><topic>Frozen brain tissue</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Human brain</topic><topic>Humans</topic><topic>Mechanical slicing</topic><topic>Microtomy</topic><topic>Tissue Preservation</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oke, A.F.</creatorcontrib><creatorcontrib>Moghaddam, M.</creatorcontrib><creatorcontrib>Ayetey, W.E.A.</creatorcontrib><creatorcontrib>Adams, R.N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oke, A.F.</au><au>Moghaddam, M.</au><au>Ayetey, W.E.A.</au><au>Adams, R.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical slicing of frozen brain tissue: a reappraisal of catecholamine loss</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1987-11-01</date><risdate>1987</risdate><volume>22</volume><issue>1</issue><spage>41</spage><epage>46</epage><pages>41-46</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>Catecholamine levels in mechanically sliced frozen bovine brain tissue were compared with those found in hand-cut, unfrozen tissue. Hemispheric content of catecholamines in small regions of cortex, caudate, nucleus accumbens, thalamus, and hippocampus in bovine brain was no different from that seen in the homologous area of the contralateral hemisphere, despite selective slicing of opposing hemispheres with the two techniques. Brodmann area 25 in human brain (previously suspected to be highly vulnerable to degradative loss of catecholamines by mechanical slicing) is shown to be a restricted area of dense catecholamine concentration when compared to surrounding cortical tissue. The usage of Brodmann area 25 for technique comparisons without precise control of cortical delineation is questioned.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3695566</pmid><doi>10.1016/0165-0270(87)90087-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of neuroscience methods, 1987-11, Vol.22 (1), p.41-46 |
| issn | 0165-0270 1872-678X |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Brain Chemistry Catecholamine content Catecholamines - analysis Cattle Frozen brain tissue Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Human brain Humans Mechanical slicing Microtomy Tissue Preservation Vertebrates: nervous system and sense organs |
| title | Mechanical slicing of frozen brain tissue: a reappraisal of catecholamine loss |
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