CMP-NeuAc:(NeuAc alpha 2-->8)n (colominic acid) sialyltransferase activity in rat brain and in tumour cells that express polysialic acid on neural cell adhesion molecules

A method for the assay of CMP-NeuAc:(NeuAc alpha 2-->8)n (colominic acid) sialyltransferase activity was developed. Using a 1-day-old rat brain membrane fraction as an enzyme preparation optimal activity was obtained at pH 6.5, 0.3% Triton X-100, and 5 mM MnCl2. However, no absolute cation requir...

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Veröffentlicht in:	Glycoconjugate journal 1995-12, Vol.12 (6), p.829-837
Hauptverfasser:	Easton, E W, Schiphorst, W E, Koeleman, C A, Michalides, R J, Van Den Eijnden, D H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain - enzymology Brain - growth & development Carcinoma, Small Cell - enzymology Cell Line Kinetics Lung Neoplasms - enzymology Neural Cell Adhesion Molecules - chemistry Neuroblastoma - enzymology Polysaccharides - analysis Rats Sialic Acids - analysis Sialyltransferases - analysis Tumor Cells, Cultured
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