Comparison between a swim-up and a Percoll gradient technique for the separation of human spermatozoa

Comparison between a swim-up and a Percoll gradient technique for the separation of human spermatozoa

Summary Two methods of separating human sperm were compared using twenty‐two semen samples. The sperm were separated by a swim‐up technique or by self‐migration on a Percoll gradient followed by medium change. After separation, the sperm obtained were assessed for progressive motility, ATP content,...

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Veröffentlicht in:International journal of andrology 1987-10, Vol.10 (5), p.663-669
Hauptverfasser: ÅKERLÖF, EVA, FREDRICSON, B., GUSTAFSSON, O., LUNDIN, A., LUNELL, N. O., NYLUND, L., ROSENBORG, L., POUSETTE, Å.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphate - analysis
ATP content
Biological and medical sciences
Birth control
Cytological Techniques
energy charge index
Gynecology. Andrology. Obstetrics
Humans
Male
Medical sciences
morphology
Percoll gradients
Semen - analysis
Semen - cytology
Semen - physiology
Sperm Count
Sperm Motility
spermatozoa
Spermatozoa - analysis
Sterility. Assisted procreation
swim-up technique
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Zusammenfassung:Summary Two methods of separating human sperm were compared using twenty‐two semen samples. The sperm were separated by a swim‐up technique or by self‐migration on a Percoll gradient followed by medium change. After separation, the sperm obtained were assessed for progressive motility, ATP content, energy charge index ([ATP + 0.5 ADP]/[ATP + ADP + AMP]) and morphology. In general, and especially for semen samples containing
ISSN:0105-6263
1365-2605
DOI:10.1111/j.1365-2605.1987.tb00367.x