Effect of bezafibrate on lipoprotein secretion by cultured human hepatocytes
The secretion of newly synthesized very low density lipoprotein and low density lipoprotein (VLDL + LDL) and high density lipoprotein (HDL) in cultured human hepatocytes in the presence or absence of bezafibrate added to the culture medium has been evaluated. The content of triacylglycerol, apolipop...
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| Veröffentlicht in: | Atherosclerosis 1987-11, Vol.68 (1), p.67-76 |
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| container_title | Atherosclerosis |
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| creator | Kosykh, Vladimir A. Podrez, Eugeniy A. Novikov, Dmitriy K. Victorov, Alexander V. Dolbin, Anatoliy G. Repin, Vadim S. Smirnov, Vladimir N. |
| description | The secretion of newly synthesized very low density lipoprotein and low density lipoprotein (VLDL + LDL) and high density lipoprotein (HDL) in cultured human hepatocytes in the presence or absence of bezafibrate added to the culture medium has been evaluated. The content of triacylglycerol, apolipoprotein B,
3H-labeled proteins and
14C-labeled lipids accumulated in a culture medium increased linearly for periods up to 18 h. During incubation, cellular triacylglycerol content was unchanged. This hypolipidemic agent, at a concentration of 10 μM, inhibited secretion of the several VLDL + LDL [3H]apolipoproteins and the VLDL + LDL [
14C]lipids, suggesting it can affect the processes of biosynthesis and secretion. The secretion of total [
3H]apolipoproteins in the HDL fraction and [3H]protein (
d > 1.21 g/ml) was unchanged after an exposure to bezafibrate (10 μM). Incubation in the presence of 10 tLM bezafibrate resulted also in decreases in both total VLDL neutral lipids and apolipoprotein B secreted into the medium.
These results indicate that bezafibrate might exert its plasma lipid-lowering effect by suppressing the VLDL production in liver and reducing the secretion of VLDL into the circulation. |
| doi_str_mv | 10.1016/0021-9150(87)90095-5 |
| format | Article |
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3H-labeled proteins and
14C-labeled lipids accumulated in a culture medium increased linearly for periods up to 18 h. During incubation, cellular triacylglycerol content was unchanged. This hypolipidemic agent, at a concentration of 10 μM, inhibited secretion of the several VLDL + LDL [3H]apolipoproteins and the VLDL + LDL [
14C]lipids, suggesting it can affect the processes of biosynthesis and secretion. The secretion of total [
3H]apolipoproteins in the HDL fraction and [3H]protein (
d > 1.21 g/ml) was unchanged after an exposure to bezafibrate (10 μM). Incubation in the presence of 10 tLM bezafibrate resulted also in decreases in both total VLDL neutral lipids and apolipoprotein B secreted into the medium.
These results indicate that bezafibrate might exert its plasma lipid-lowering effect by suppressing the VLDL production in liver and reducing the secretion of VLDL into the circulation.</description><identifier>ISSN: 0021-9150</identifier><identifier>EISSN: 1879-1484</identifier><identifier>DOI: 10.1016/0021-9150(87)90095-5</identifier><identifier>PMID: 3689484</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ireland Ltd</publisher><subject>Bezafibrate ; Bezafibrate - pharmacology ; Biological and medical sciences ; Cells, Cultured ; General and cellular metabolism. Vitamins ; HDL ; Human hepatocytes ; Humans ; Lipoprotein secretion ; Lipoproteins - biosynthesis ; Lipoproteins - metabolism ; Lipoproteins, HDL - metabolism ; Lipoproteins, LDL - metabolism ; Lipoproteins, VLDL - biosynthesis ; Lipoproteins, VLDL - metabolism ; Liver - drug effects ; Liver - metabolism ; Medical sciences ; Pharmacology. Drug treatments ; VLDL</subject><ispartof>Atherosclerosis, 1987-11, Vol.68 (1), p.67-76</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4e1e414e895cffca48b596921c6677c49a35ecb3b70861c14c20e1d093a3fdc13</citedby><cites>FETCH-LOGICAL-c386t-4e1e414e895cffca48b596921c6677c49a35ecb3b70861c14c20e1d093a3fdc13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0021-9150(87)90095-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7831648$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3689484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kosykh, Vladimir A.</creatorcontrib><creatorcontrib>Podrez, Eugeniy A.</creatorcontrib><creatorcontrib>Novikov, Dmitriy K.</creatorcontrib><creatorcontrib>Victorov, Alexander V.</creatorcontrib><creatorcontrib>Dolbin, Anatoliy G.</creatorcontrib><creatorcontrib>Repin, Vadim S.</creatorcontrib><creatorcontrib>Smirnov, Vladimir N.</creatorcontrib><title>Effect of bezafibrate on lipoprotein secretion by cultured human hepatocytes</title><title>Atherosclerosis</title><addtitle>Atherosclerosis</addtitle><description>The secretion of newly synthesized very low density lipoprotein and low density lipoprotein (VLDL + LDL) and high density lipoprotein (HDL) in cultured human hepatocytes in the presence or absence of bezafibrate added to the culture medium has been evaluated. The content of triacylglycerol, apolipoprotein B,
3H-labeled proteins and
14C-labeled lipids accumulated in a culture medium increased linearly for periods up to 18 h. During incubation, cellular triacylglycerol content was unchanged. This hypolipidemic agent, at a concentration of 10 μM, inhibited secretion of the several VLDL + LDL [3H]apolipoproteins and the VLDL + LDL [
14C]lipids, suggesting it can affect the processes of biosynthesis and secretion. The secretion of total [
3H]apolipoproteins in the HDL fraction and [3H]protein (
d > 1.21 g/ml) was unchanged after an exposure to bezafibrate (10 μM). Incubation in the presence of 10 tLM bezafibrate resulted also in decreases in both total VLDL neutral lipids and apolipoprotein B secreted into the medium.
These results indicate that bezafibrate might exert its plasma lipid-lowering effect by suppressing the VLDL production in liver and reducing the secretion of VLDL into the circulation.</description><subject>Bezafibrate</subject><subject>Bezafibrate - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>General and cellular metabolism. Vitamins</subject><subject>HDL</subject><subject>Human hepatocytes</subject><subject>Humans</subject><subject>Lipoprotein secretion</subject><subject>Lipoproteins - biosynthesis</subject><subject>Lipoproteins - metabolism</subject><subject>Lipoproteins, HDL - metabolism</subject><subject>Lipoproteins, LDL - metabolism</subject><subject>Lipoproteins, VLDL - biosynthesis</subject><subject>Lipoproteins, VLDL - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>VLDL</subject><issn>0021-9150</issn><issn>1879-1484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVocB0n_6CFPZTSHjbReLX6uASKcZqAIZf2LLSzI6Ky3nUlbcD59VnXxseeBuZ9Znh5GPsE_BY4yDvOl1AaqPk3rb4bzk1d1hdsDlqZEoQWH9j8jHxkVyn94ZwLBXrGZpXUZkLmbLP2njAXgy8aenM-NNFlKoa-6MJu2MUhU-iLRBgph2nb7AscuzxGaouXcev64oV2Lg-4z5Su2aV3XaKb01yw3w_rX6vHcvP882n1Y1NipWUuBQEJEKRNjd6jE7qpjTRLQCmVQmFcVRM2VaO4loAgcMkJWm4qV_kWoVqwr8e_U7-_I6VstyEhdZ3raRiTVUprIZf1BIojiHFIKZK3uxi2Lu4tcHuQaA-G7MGQ1cr-k2gPZ59P_8dmS-356GRtyr-ccpfQdT66HkM6Y0pXIIWesPsjRpOL10DRJgzUI7UhTs5tO4T_93gHa4eN2g</recordid><startdate>19871101</startdate><enddate>19871101</enddate><creator>Kosykh, Vladimir A.</creator><creator>Podrez, Eugeniy A.</creator><creator>Novikov, Dmitriy K.</creator><creator>Victorov, Alexander V.</creator><creator>Dolbin, Anatoliy G.</creator><creator>Repin, Vadim S.</creator><creator>Smirnov, Vladimir N.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19871101</creationdate><title>Effect of bezafibrate on lipoprotein secretion by cultured human hepatocytes</title><author>Kosykh, Vladimir A. ; Podrez, Eugeniy A. ; Novikov, Dmitriy K. ; Victorov, Alexander V. ; Dolbin, Anatoliy G. ; Repin, Vadim S. ; Smirnov, Vladimir N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-4e1e414e895cffca48b596921c6677c49a35ecb3b70861c14c20e1d093a3fdc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Bezafibrate</topic><topic>Bezafibrate - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>General and cellular metabolism. Vitamins</topic><topic>HDL</topic><topic>Human hepatocytes</topic><topic>Humans</topic><topic>Lipoprotein secretion</topic><topic>Lipoproteins - biosynthesis</topic><topic>Lipoproteins - metabolism</topic><topic>Lipoproteins, HDL - metabolism</topic><topic>Lipoproteins, LDL - metabolism</topic><topic>Lipoproteins, VLDL - biosynthesis</topic><topic>Lipoproteins, VLDL - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>VLDL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kosykh, Vladimir A.</creatorcontrib><creatorcontrib>Podrez, Eugeniy A.</creatorcontrib><creatorcontrib>Novikov, Dmitriy K.</creatorcontrib><creatorcontrib>Victorov, Alexander V.</creatorcontrib><creatorcontrib>Dolbin, Anatoliy G.</creatorcontrib><creatorcontrib>Repin, Vadim S.</creatorcontrib><creatorcontrib>Smirnov, Vladimir N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Atherosclerosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kosykh, Vladimir A.</au><au>Podrez, Eugeniy A.</au><au>Novikov, Dmitriy K.</au><au>Victorov, Alexander V.</au><au>Dolbin, Anatoliy G.</au><au>Repin, Vadim S.</au><au>Smirnov, Vladimir N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of bezafibrate on lipoprotein secretion by cultured human hepatocytes</atitle><jtitle>Atherosclerosis</jtitle><addtitle>Atherosclerosis</addtitle><date>1987-11-01</date><risdate>1987</risdate><volume>68</volume><issue>1</issue><spage>67</spage><epage>76</epage><pages>67-76</pages><issn>0021-9150</issn><eissn>1879-1484</eissn><abstract>The secretion of newly synthesized very low density lipoprotein and low density lipoprotein (VLDL + LDL) and high density lipoprotein (HDL) in cultured human hepatocytes in the presence or absence of bezafibrate added to the culture medium has been evaluated. The content of triacylglycerol, apolipoprotein B,
3H-labeled proteins and
14C-labeled lipids accumulated in a culture medium increased linearly for periods up to 18 h. During incubation, cellular triacylglycerol content was unchanged. This hypolipidemic agent, at a concentration of 10 μM, inhibited secretion of the several VLDL + LDL [3H]apolipoproteins and the VLDL + LDL [
14C]lipids, suggesting it can affect the processes of biosynthesis and secretion. The secretion of total [
3H]apolipoproteins in the HDL fraction and [3H]protein (
d > 1.21 g/ml) was unchanged after an exposure to bezafibrate (10 μM). Incubation in the presence of 10 tLM bezafibrate resulted also in decreases in both total VLDL neutral lipids and apolipoprotein B secreted into the medium.
These results indicate that bezafibrate might exert its plasma lipid-lowering effect by suppressing the VLDL production in liver and reducing the secretion of VLDL into the circulation.</abstract><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>3689484</pmid><doi>10.1016/0021-9150(87)90095-5</doi><tpages>10</tpages></addata></record> |
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| ispartof | Atherosclerosis, 1987-11, Vol.68 (1), p.67-76 |
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| subjects | Bezafibrate Bezafibrate - pharmacology Biological and medical sciences Cells, Cultured General and cellular metabolism. Vitamins HDL Human hepatocytes Humans Lipoprotein secretion Lipoproteins - biosynthesis Lipoproteins - metabolism Lipoproteins, HDL - metabolism Lipoproteins, LDL - metabolism Lipoproteins, VLDL - biosynthesis Lipoproteins, VLDL - metabolism Liver - drug effects Liver - metabolism Medical sciences Pharmacology. Drug treatments VLDL |
| title | Effect of bezafibrate on lipoprotein secretion by cultured human hepatocytes |
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