Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5)
By immunizing mice genetically deficient in C5 we were able to obtain a group of monoclonal antibodies to rabbit C5 that cross-react with C5 from a wide variety of mammalian sera, including mouse. The specificity of the monoclonal antibodies was against native C5 and C5b but not C5a. The antibodies...
Gespeichert in:
| Veröffentlicht in: | Journal of immunological methods 1987-12, Vol.105 (2), p.201-209 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 209 |
|---|---|
| container_issue | 2 |
| container_start_page | 201 |
| container_title | Journal of immunological methods |
| container_volume | 105 |
| creator | Giclas, P.C. Baker, S.L. Gillespie, M.L. Wilcox, C. |
| description | By immunizing mice genetically deficient in C5 we were able to obtain a group of monoclonal antibodies to rabbit C5 that cross-react with C5 from a wide variety of mammalian sera, including mouse. The specificity of the monoclonal antibodies was against native C5 and C5b but not C5a. The antibodies strongly inhibit the expression of C5 hemolytic activity. We suggest that these monoclonal antibodies will be useful for studying C5 as well as providing a way to selectively deplete C5 from plasma in vitro or in vivo. |
| doi_str_mv | 10.1016/0022-1759(87)90267-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77881756</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0022175987902675</els_id><sourcerecordid>77881756</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-a80d5d36d5791194609e894df83bac9365eb66bcee20850c85e41e12ebf938183</originalsourceid><addsrcrecordid>eNp9kF1rFDEUhoO0tGv1HyjMRZH2YvRksvm6EWRRKxT0Qq9DJjnjRmaSbZIV6q93prvsZa9C3vO8h8NDyBsK7ylQ8QGg61oqub5R8lZDJ2TLX5AVVbJrpQZ-RlYn5JK8LOUPAFAQcEEumNBMg1qR9CPjzmZbQ4qNjb5x2_nnKubw7xCmoZlSTG5M0Y4zUkOffMDS2N82xFKbusVmCEPdNi5NuxQx1qWUbd-H-pSNOC3hzYbfviLngx0Lvj6-V-TXl88_N3ft_fev3zaf7lvHgNbWKvDcM-G51JTqtQCNSq_9oFhvnWaCYy9E7xA7UByc4rimSDvsB80UVeyKvDvs3eX0sMdSzRSKw3G0EdO-GCmVmsWIGVwfQJdTKRkHs8thsvnRUDCLZ7NINItEo6R58mz4XHt73L_vJ_Sn0lHsPL8-zm1xdhyyjS6UEyZBMt3pGft4wHB28TdgNsUFjA59yOiq8Sk8f8d_bEGaEg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77881756</pqid></control><display><type>article</type><title>Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5)</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Giclas, P.C. ; Baker, S.L. ; Gillespie, M.L. ; Wilcox, C.</creator><creatorcontrib>Giclas, P.C. ; Baker, S.L. ; Gillespie, M.L. ; Wilcox, C.</creatorcontrib><description>By immunizing mice genetically deficient in C5 we were able to obtain a group of monoclonal antibodies to rabbit C5 that cross-react with C5 from a wide variety of mammalian sera, including mouse. The specificity of the monoclonal antibodies was against native C5 and C5b but not C5a. The antibodies strongly inhibit the expression of C5 hemolytic activity. We suggest that these monoclonal antibodies will be useful for studying C5 as well as providing a way to selectively deplete C5 from plasma in vitro or in vivo.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(87)90267-5</identifier><identifier>PMID: 3693908</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Applied sciences ; Biological Assay ; Chemical Precipitation ; Complement C5 - immunology ; Complement C5a ; Complement C5b ; Complement, rabbit ; Cross Reactions ; Exact sciences and technology ; Molecular Weight ; Monoclonal antibody ; Other techniques and industries ; Rabbits</subject><ispartof>Journal of immunological methods, 1987-12, Vol.105 (2), p.201-209</ispartof><rights>1987</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-a80d5d36d5791194609e894df83bac9365eb66bcee20850c85e41e12ebf938183</citedby><cites>FETCH-LOGICAL-c301t-a80d5d36d5791194609e894df83bac9365eb66bcee20850c85e41e12ebf938183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(87)90267-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7073929$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3693908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giclas, P.C.</creatorcontrib><creatorcontrib>Baker, S.L.</creatorcontrib><creatorcontrib>Gillespie, M.L.</creatorcontrib><creatorcontrib>Wilcox, C.</creatorcontrib><title>Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5)</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>By immunizing mice genetically deficient in C5 we were able to obtain a group of monoclonal antibodies to rabbit C5 that cross-react with C5 from a wide variety of mammalian sera, including mouse. The specificity of the monoclonal antibodies was against native C5 and C5b but not C5a. The antibodies strongly inhibit the expression of C5 hemolytic activity. We suggest that these monoclonal antibodies will be useful for studying C5 as well as providing a way to selectively deplete C5 from plasma in vitro or in vivo.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Applied sciences</subject><subject>Biological Assay</subject><subject>Chemical Precipitation</subject><subject>Complement C5 - immunology</subject><subject>Complement C5a</subject><subject>Complement C5b</subject><subject>Complement, rabbit</subject><subject>Cross Reactions</subject><subject>Exact sciences and technology</subject><subject>Molecular Weight</subject><subject>Monoclonal antibody</subject><subject>Other techniques and industries</subject><subject>Rabbits</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFDEUhoO0tGv1HyjMRZH2YvRksvm6EWRRKxT0Qq9DJjnjRmaSbZIV6q93prvsZa9C3vO8h8NDyBsK7ylQ8QGg61oqub5R8lZDJ2TLX5AVVbJrpQZ-RlYn5JK8LOUPAFAQcEEumNBMg1qR9CPjzmZbQ4qNjb5x2_nnKubw7xCmoZlSTG5M0Y4zUkOffMDS2N82xFKbusVmCEPdNi5NuxQx1qWUbd-H-pSNOC3hzYbfviLngx0Lvj6-V-TXl88_N3ft_fev3zaf7lvHgNbWKvDcM-G51JTqtQCNSq_9oFhvnWaCYy9E7xA7UByc4rimSDvsB80UVeyKvDvs3eX0sMdSzRSKw3G0EdO-GCmVmsWIGVwfQJdTKRkHs8thsvnRUDCLZ7NINItEo6R58mz4XHt73L_vJ_Sn0lHsPL8-zm1xdhyyjS6UEyZBMt3pGft4wHB28TdgNsUFjA59yOiq8Sk8f8d_bEGaEg</recordid><startdate>19871224</startdate><enddate>19871224</enddate><creator>Giclas, P.C.</creator><creator>Baker, S.L.</creator><creator>Gillespie, M.L.</creator><creator>Wilcox, C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19871224</creationdate><title>Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5)</title><author>Giclas, P.C. ; Baker, S.L. ; Gillespie, M.L. ; Wilcox, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-a80d5d36d5791194609e894df83bac9365eb66bcee20850c85e41e12ebf938183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Applied sciences</topic><topic>Biological Assay</topic><topic>Chemical Precipitation</topic><topic>Complement C5 - immunology</topic><topic>Complement C5a</topic><topic>Complement C5b</topic><topic>Complement, rabbit</topic><topic>Cross Reactions</topic><topic>Exact sciences and technology</topic><topic>Molecular Weight</topic><topic>Monoclonal antibody</topic><topic>Other techniques and industries</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giclas, P.C.</creatorcontrib><creatorcontrib>Baker, S.L.</creatorcontrib><creatorcontrib>Gillespie, M.L.</creatorcontrib><creatorcontrib>Wilcox, C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giclas, P.C.</au><au>Baker, S.L.</au><au>Gillespie, M.L.</au><au>Wilcox, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5)</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1987-12-24</date><risdate>1987</risdate><volume>105</volume><issue>2</issue><spage>201</spage><epage>209</epage><pages>201-209</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>By immunizing mice genetically deficient in C5 we were able to obtain a group of monoclonal antibodies to rabbit C5 that cross-react with C5 from a wide variety of mammalian sera, including mouse. The specificity of the monoclonal antibodies was against native C5 and C5b but not C5a. The antibodies strongly inhibit the expression of C5 hemolytic activity. We suggest that these monoclonal antibodies will be useful for studying C5 as well as providing a way to selectively deplete C5 from plasma in vitro or in vivo.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3693908</pmid><doi>10.1016/0022-1759(87)90267-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 1987-12, Vol.105 (2), p.201-209 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77881756 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies, Monoclonal - immunology Applied sciences Biological Assay Chemical Precipitation Complement C5 - immunology Complement C5a Complement C5b Complement, rabbit Cross Reactions Exact sciences and technology Molecular Weight Monoclonal antibody Other techniques and industries Rabbits |
| title | Preparation and characterization of monoclonal antibodies against the fifth component of rabbit complement (C5) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T20%3A07%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20characterization%20of%20monoclonal%20antibodies%20against%20the%20fifth%20component%20of%20rabbit%20complement%20(C5)&rft.jtitle=Journal%20of%20immunological%20methods&rft.au=Giclas,%20P.C.&rft.date=1987-12-24&rft.volume=105&rft.issue=2&rft.spage=201&rft.epage=209&rft.pages=201-209&rft.issn=0022-1759&rft.eissn=1872-7905&rft.coden=JIMMBG&rft_id=info:doi/10.1016/0022-1759(87)90267-5&rft_dat=%3Cproquest_cross%3E77881756%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77881756&rft_id=info:pmid/3693908&rft_els_id=0022175987902675&rfr_iscdi=true |