Determination of 2-(9-Anthryl)ethyl Chloroformate-Labeled Amino Acids by Capillary Electrophoresis and Liquid Chromatography with Absorbance or Fluorescence Detection
A new precolumn reagent for amino acid determination, 2-(9-anthryl)ethyl chloroformate (AEOC), was introduced to obtain higher sensitivity in two capillary separation techniques, liquid chromatography (LC) and electrophoresis (CE). The chromophore in the (9-fluorenyl)methyl chloroformate (FMOC) reag...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1995-09, Vol.67 (17), p.3018-3022 |
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| creator | Engstroem, Anders Andersson, Per E Josefsson, Bjoern Pfeffer, William D |
| description | A new precolumn reagent for amino acid determination, 2-(9-anthryl)ethyl chloroformate (AEOC), was introduced to obtain higher sensitivity in two capillary separation techniques, liquid chromatography (LC) and electrophoresis (CE). The chromophore in the (9-fluorenyl)methyl chloroformate (FMOC) reagent was replaced by anthracene, which resulted in a reagent with very high molar absorptivity (epsilon 256 = 180,000 L mol-1 cm-1). This permits AEOC-tagged species to be detected at nanomolar levels with UV absorbance detection in standard 50-microns-i.d. fused silica capillaries. Weaker absorption bands match the UV argon laser lines of 351 and 368 nm, which allows for convenient laser-induced fluorescence (LIF) detection. In this mode, picomolar limits of detection are obtained. In addition to measuring the limits of detection, we examined micellar electrokinetic chromatography, free solution capillary electrophoresis, and packed capillary LC and compared these methods regarding the separation of amino acids. |
| doi_str_mv | 10.1021/ac00113a041 |
| format | Article |
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The chromophore in the (9-fluorenyl)methyl chloroformate (FMOC) reagent was replaced by anthracene, which resulted in a reagent with very high molar absorptivity (epsilon 256 = 180,000 L mol-1 cm-1). This permits AEOC-tagged species to be detected at nanomolar levels with UV absorbance detection in standard 50-microns-i.d. fused silica capillaries. Weaker absorption bands match the UV argon laser lines of 351 and 368 nm, which allows for convenient laser-induced fluorescence (LIF) detection. In this mode, picomolar limits of detection are obtained. In addition to measuring the limits of detection, we examined micellar electrokinetic chromatography, free solution capillary electrophoresis, and packed capillary LC and compared these methods regarding the separation of amino acids.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00113a041</identifier><identifier>PMID: 8779422</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Amino acids ; Amino Acids - analysis ; Amino Acids - isolation & purification ; Analytical chemistry ; Anthracenes - chemistry ; Biochemistry ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Liquid ; Electrophoresis, Capillary ; Exact sciences and technology ; Formic Acid Esters - chemistry ; Indicators and Reagents ; Other chromatographic methods ; Spectrometry, Fluorescence</subject><ispartof>Analytical chemistry (Washington), 1995-09, Vol.67 (17), p.3018-3022</ispartof><rights>1995 INIST-CNRS</rights><rights>Copyright American Chemical Society Sep 1, 1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a325t-dca3abe80d3e8a41316f7fe9354cfe6025c5a729c99fa1375de2e7d5e86597923</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac00113a041$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac00113a041$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3637581$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8779422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Engstroem, Anders</creatorcontrib><creatorcontrib>Andersson, Per E</creatorcontrib><creatorcontrib>Josefsson, Bjoern</creatorcontrib><creatorcontrib>Pfeffer, William D</creatorcontrib><title>Determination of 2-(9-Anthryl)ethyl Chloroformate-Labeled Amino Acids by Capillary Electrophoresis and Liquid Chromatography with Absorbance or Fluorescence Detection</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A new precolumn reagent for amino acid determination, 2-(9-anthryl)ethyl chloroformate (AEOC), was introduced to obtain higher sensitivity in two capillary separation techniques, liquid chromatography (LC) and electrophoresis (CE). The chromophore in the (9-fluorenyl)methyl chloroformate (FMOC) reagent was replaced by anthracene, which resulted in a reagent with very high molar absorptivity (epsilon 256 = 180,000 L mol-1 cm-1). This permits AEOC-tagged species to be detected at nanomolar levels with UV absorbance detection in standard 50-microns-i.d. fused silica capillaries. Weaker absorption bands match the UV argon laser lines of 351 and 368 nm, which allows for convenient laser-induced fluorescence (LIF) detection. In this mode, picomolar limits of detection are obtained. In addition to measuring the limits of detection, we examined micellar electrokinetic chromatography, free solution capillary electrophoresis, and packed capillary LC and compared these methods regarding the separation of amino acids.</description><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Amino Acids - isolation & purification</subject><subject>Analytical chemistry</subject><subject>Anthracenes - chemistry</subject><subject>Biochemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Liquid</subject><subject>Electrophoresis, Capillary</subject><subject>Exact sciences and technology</subject><subject>Formic Acid Esters - chemistry</subject><subject>Indicators and Reagents</subject><subject>Other chromatographic methods</subject><subject>Spectrometry, Fluorescence</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkVuL1TAUhYMo43H0yWchiHhBqrm0TftYjjPj5YCCo4gvIU13bca06SQt2j_k7zTlHI4iPoWwv71Yay-E7lPyghJGXypNCKVckZTeQBuaMZLkRcFuog0hhCdMEHIb3QnhasUIzU_QSSFEmTK2Qb9ewQS-N4OajBuwazFLnpZJNUydX-wzmLrF4m1nnXet872aINmpGiw0uIpbDlfaNAHXC96q0Vir_ILPLOjJu7FzHoIJWA0N3pnr2TRRybso4r55NXYL_mGmDld1cL5WgwbsPD6387qmYf2v5vRq7C661Sob4N7hPUWfzs8ut6-T3fuLN9tqlyjOsilptOLRXUEaDoVKKad5K1ooeZbqFnLCMp0pwUpdlq2iXGQNMBBNBkWelaJk_BQ93uuO3l3PECbZm-gl5hrAzUEKUYiU5iv48B_wys1-iN4ko6JIC0Z5hJ7vIe1dCB5aOXrTxxNJSuRanfyrukg_OEjOdQ_NkT10FeePDnMVtLKtjycz4YjxPOYpVplkj5kwwc_jWPnvMhcRkZcfPsqvF-9Y-fYLk58j_2TPKx3-hPifwd87Hr4U</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Engstroem, Anders</creator><creator>Andersson, Per E</creator><creator>Josefsson, Bjoern</creator><creator>Pfeffer, William D</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Determination of 2-(9-Anthryl)ethyl Chloroformate-Labeled Amino Acids by Capillary Electrophoresis and Liquid Chromatography with Absorbance or Fluorescence Detection</title><author>Engstroem, Anders ; Andersson, Per E ; Josefsson, Bjoern ; Pfeffer, William D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a325t-dca3abe80d3e8a41316f7fe9354cfe6025c5a729c99fa1375de2e7d5e86597923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Amino Acids - isolation & purification</topic><topic>Analytical chemistry</topic><topic>Anthracenes - chemistry</topic><topic>Biochemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Liquid</topic><topic>Electrophoresis, Capillary</topic><topic>Exact sciences and technology</topic><topic>Formic Acid Esters - chemistry</topic><topic>Indicators and Reagents</topic><topic>Other chromatographic methods</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Engstroem, Anders</creatorcontrib><creatorcontrib>Andersson, Per E</creatorcontrib><creatorcontrib>Josefsson, Bjoern</creatorcontrib><creatorcontrib>Pfeffer, William D</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Engstroem, Anders</au><au>Andersson, Per E</au><au>Josefsson, Bjoern</au><au>Pfeffer, William D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of 2-(9-Anthryl)ethyl Chloroformate-Labeled Amino Acids by Capillary Electrophoresis and Liquid Chromatography with Absorbance or Fluorescence Detection</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>67</volume><issue>17</issue><spage>3018</spage><epage>3022</epage><pages>3018-3022</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A new precolumn reagent for amino acid determination, 2-(9-anthryl)ethyl chloroformate (AEOC), was introduced to obtain higher sensitivity in two capillary separation techniques, liquid chromatography (LC) and electrophoresis (CE). The chromophore in the (9-fluorenyl)methyl chloroformate (FMOC) reagent was replaced by anthracene, which resulted in a reagent with very high molar absorptivity (epsilon 256 = 180,000 L mol-1 cm-1). This permits AEOC-tagged species to be detected at nanomolar levels with UV absorbance detection in standard 50-microns-i.d. fused silica capillaries. Weaker absorption bands match the UV argon laser lines of 351 and 368 nm, which allows for convenient laser-induced fluorescence (LIF) detection. In this mode, picomolar limits of detection are obtained. In addition to measuring the limits of detection, we examined micellar electrokinetic chromatography, free solution capillary electrophoresis, and packed capillary LC and compared these methods regarding the separation of amino acids.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>8779422</pmid><doi>10.1021/ac00113a041</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1995-09, Vol.67 (17), p.3018-3022 |
| issn | 0003-2700 1520-6882 |
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| source | MEDLINE; ACS Publications |
| subjects | Amino acids Amino Acids - analysis Amino Acids - isolation & purification Analytical chemistry Anthracenes - chemistry Biochemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Liquid Electrophoresis, Capillary Exact sciences and technology Formic Acid Esters - chemistry Indicators and Reagents Other chromatographic methods Spectrometry, Fluorescence |
| title | Determination of 2-(9-Anthryl)ethyl Chloroformate-Labeled Amino Acids by Capillary Electrophoresis and Liquid Chromatography with Absorbance or Fluorescence Detection |
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