Evidence for the presence of low density and very low density lipoproteins in human amniotic fluid
Previous analysis of amniotic fluid (AF) noted only the presence of high density lipoprotein (HDL). In this study AF lipoprotein profile was examined using gel filtration column chromatography and Ouchterlony gel diffusion. Unlike previous studies which showed only the presence of HDL, we found sign...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1987-11, Vol.149 (1), p.208-212 |
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| creator | Park, M-S.C. Subbiah, M.T.R. |
| description | Previous analysis of amniotic fluid (AF) noted only the presence of high density lipoprotein (HDL). In this study AF lipoprotein profile was examined using gel filtration column chromatography and Ouchterlony gel diffusion. Unlike previous studies which showed only the presence of HDL, we found significant amounts of low density lipoprotein (LDL) and very low density lipoprotein (VLDL). AF-LDL and AF-VLDL were identified by reactions with anti-h-apolipoprotein AI and AII antiserum and anti-h-apolipoprotein B-antiserum, respectively. Furthermore, bulk of the cholesterol mass was carried in VLDL (53.6 ± 7.7%) and LDL (32.5 ± 4.3%) with minor amounts (13.9 ± 1.3%) in HDL fraction. It is concluded that human AF contains all three lipoproteins with most of the cholesterol being carried in very low density lipoprotein fraction. |
| doi_str_mv | 10.1016/0006-291X(87)91625-1 |
| format | Article |
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In this study AF lipoprotein profile was examined using gel filtration column chromatography and Ouchterlony gel diffusion. Unlike previous studies which showed only the presence of HDL, we found significant amounts of low density lipoprotein (LDL) and very low density lipoprotein (VLDL). AF-LDL and AF-VLDL were identified by reactions with anti-h-apolipoprotein AI and AII antiserum and anti-h-apolipoprotein B-antiserum, respectively. Furthermore, bulk of the cholesterol mass was carried in VLDL (53.6 ± 7.7%) and LDL (32.5 ± 4.3%) with minor amounts (13.9 ± 1.3%) in HDL fraction. It is concluded that human AF contains all three lipoproteins with most of the cholesterol being carried in very low density lipoprotein fraction.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(87)91625-1</identifier><identifier>PMID: 3120724</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amniotic Fluid - analysis ; Applied sciences ; Cholesterol - analysis ; Chromatography, Gel ; Exact sciences and technology ; Humans ; Immunodiffusion ; Lipoproteins, LDL - analysis ; Lipoproteins, VLDL - analysis ; Other techniques and industries</subject><ispartof>Biochemical and biophysical research communications, 1987-11, Vol.149 (1), p.208-212</ispartof><rights>1987</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4afeab6330fddaa21f17708504c83a0edf46eccddbe0253a0f99b0d0fb5345d43</citedby><cites>FETCH-LOGICAL-c386t-4afeab6330fddaa21f17708504c83a0edf46eccddbe0253a0f99b0d0fb5345d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(87)91625-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7073631$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3120724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, M-S.C.</creatorcontrib><creatorcontrib>Subbiah, M.T.R.</creatorcontrib><title>Evidence for the presence of low density and very low density lipoproteins in human amniotic fluid</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Previous analysis of amniotic fluid (AF) noted only the presence of high density lipoprotein (HDL). In this study AF lipoprotein profile was examined using gel filtration column chromatography and Ouchterlony gel diffusion. Unlike previous studies which showed only the presence of HDL, we found significant amounts of low density lipoprotein (LDL) and very low density lipoprotein (VLDL). AF-LDL and AF-VLDL were identified by reactions with anti-h-apolipoprotein AI and AII antiserum and anti-h-apolipoprotein B-antiserum, respectively. Furthermore, bulk of the cholesterol mass was carried in VLDL (53.6 ± 7.7%) and LDL (32.5 ± 4.3%) with minor amounts (13.9 ± 1.3%) in HDL fraction. It is concluded that human AF contains all three lipoproteins with most of the cholesterol being carried in very low density lipoprotein fraction.</description><subject>Amniotic Fluid - analysis</subject><subject>Applied sciences</subject><subject>Cholesterol - analysis</subject><subject>Chromatography, Gel</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Immunodiffusion</subject><subject>Lipoproteins, LDL - analysis</subject><subject>Lipoproteins, VLDL - analysis</subject><subject>Other techniques and industries</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVoSTdp_kEKOpTSHpyMLFmyL4ESkrYQ6CWB3oQsjYiCbW0ke8P--9rZZSGXnAbeL4aHkHMGFwyYvAQAWZQN-_e9Vj8aJsuqYEdkxaCBomQgPpDVIfKJnOT8BMCYkM0xOeasBFWKFWlvNsHhYJH6mOj4iHSdML8K0dMuvtDZzWHcUjM4usG0fSN2YR3XKY4YhkzDQB-n3gzU9EOIY7DUd1Nwn8lHb7qMZ_t7Sh5ub-6vfxd3f3_9uf55V1hey7EQxqNpJefgnTOmZJ4pBXUFwtbcADovJFrrXItQVrPim6YFB76tuKic4Kfk2253fuh5wjzqPmSLXWcGjFPWStWyUZLNQbEL2hRzTuj1OoXepK1moBe0euGmF266VvoVrV5qX_b7U9ujO5T2LGf_69432ZrOJzPYkA8xBYpLvsxc7WI4s9gETDrbsAB3IaEdtYvh_T_-A-4pluY</recordid><startdate>19871130</startdate><enddate>19871130</enddate><creator>Park, M-S.C.</creator><creator>Subbiah, M.T.R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19871130</creationdate><title>Evidence for the presence of low density and very low density lipoproteins in human amniotic fluid</title><author>Park, M-S.C. ; Subbiah, M.T.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-4afeab6330fddaa21f17708504c83a0edf46eccddbe0253a0f99b0d0fb5345d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amniotic Fluid - analysis</topic><topic>Applied sciences</topic><topic>Cholesterol - analysis</topic><topic>Chromatography, Gel</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Immunodiffusion</topic><topic>Lipoproteins, LDL - analysis</topic><topic>Lipoproteins, VLDL - analysis</topic><topic>Other techniques and industries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, M-S.C.</creatorcontrib><creatorcontrib>Subbiah, M.T.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, M-S.C.</au><au>Subbiah, M.T.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for the presence of low density and very low density lipoproteins in human amniotic fluid</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1987-11-30</date><risdate>1987</risdate><volume>149</volume><issue>1</issue><spage>208</spage><epage>212</epage><pages>208-212</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Previous analysis of amniotic fluid (AF) noted only the presence of high density lipoprotein (HDL). In this study AF lipoprotein profile was examined using gel filtration column chromatography and Ouchterlony gel diffusion. Unlike previous studies which showed only the presence of HDL, we found significant amounts of low density lipoprotein (LDL) and very low density lipoprotein (VLDL). AF-LDL and AF-VLDL were identified by reactions with anti-h-apolipoprotein AI and AII antiserum and anti-h-apolipoprotein B-antiserum, respectively. Furthermore, bulk of the cholesterol mass was carried in VLDL (53.6 ± 7.7%) and LDL (32.5 ± 4.3%) with minor amounts (13.9 ± 1.3%) in HDL fraction. It is concluded that human AF contains all three lipoproteins with most of the cholesterol being carried in very low density lipoprotein fraction.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3120724</pmid><doi>10.1016/0006-291X(87)91625-1</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1987-11, Vol.149 (1), p.208-212 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amniotic Fluid - analysis Applied sciences Cholesterol - analysis Chromatography, Gel Exact sciences and technology Humans Immunodiffusion Lipoproteins, LDL - analysis Lipoproteins, VLDL - analysis Other techniques and industries |
| title | Evidence for the presence of low density and very low density lipoproteins in human amniotic fluid |
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