Detection of Mycobacterium tuberculosis complex in clinical specimens by a commercial polymerase chain reaction kit

A total of 722 respiratory and 86 nonrespiratory specimens obtained from 456 patients were tested for detection of Mycobacterium tuberculosis complex by a commercial polymerase chain reaction (PCR) kit (Amplicor, Roche Diagnostic Systems) and the results compared with those of microscopy and culture...

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Veröffentlicht in:European journal of clinical microbiology & infectious diseases 1995-12, Vol.14 (12), p.1046-1051
Hauptverfasser: STAUFFER, F, MUTSCHLECHNER, R, HASENBERGER, P, STADLBAUER, S, SCHINKO, H
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container_end_page 1051
container_issue 12
container_start_page 1046
container_title European journal of clinical microbiology & infectious diseases
container_volume 14
creator STAUFFER, F
MUTSCHLECHNER, R
HASENBERGER, P
STADLBAUER, S
SCHINKO, H
description A total of 722 respiratory and 86 nonrespiratory specimens obtained from 456 patients were tested for detection of Mycobacterium tuberculosis complex by a commercial polymerase chain reaction (PCR) kit (Amplicor, Roche Diagnostic Systems) and the results compared with those of microscopy and culture (solid and radiometric media). Respiratory and nonrespiratory specimens were analysed separately. Of the respiratory specimens, 54 were positive for Mycobacterium tuberculosis complex both in the PCR and in culture, five were positive in the PCR but negative in culture, and eight were positive in culture but negative in the PCR. Four cultures were positive for mycobacteria other than Mycobacterium tuberculosis; none of these gave a positive result in the commercial test. Resolution of discrepant results was performed by analysis of patients' clinical data. For respiratory specimens the sensitivity of the commercial test was 87.6%, the specificity 99.6%, the positive predictive value 96.6%, and the negative predictive value 98.7%. For nonrespiratory specimens the sensitivity was 60%, whereas the specificity ranged as high as 98.6%. For this group the positive predictive value was 85.7% and the negative predictive value 94.9%. When respiratory specimens are used, the commercial PCR test for detection of Mycobacterium tuberculosis complex, with its high sensitivity and specificity, is a good complementary diagnostic tool for rapid diagnosis of bronchopulmonary tuberculosis in a routine mycobacterial laboratory.
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Respiratory and nonrespiratory specimens were analysed separately. Of the respiratory specimens, 54 were positive for Mycobacterium tuberculosis complex both in the PCR and in culture, five were positive in the PCR but negative in culture, and eight were positive in culture but negative in the PCR. Four cultures were positive for mycobacteria other than Mycobacterium tuberculosis; none of these gave a positive result in the commercial test. Resolution of discrepant results was performed by analysis of patients' clinical data. For respiratory specimens the sensitivity of the commercial test was 87.6%, the specificity 99.6%, the positive predictive value 96.6%, and the negative predictive value 98.7%. For nonrespiratory specimens the sensitivity was 60%, whereas the specificity ranged as high as 98.6%. For this group the positive predictive value was 85.7% and the negative predictive value 94.9%. 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source MEDLINE; SpringerNature Journals
subjects Adult
Aged
Bacterial diseases
Bacteriological Techniques - standards
Biological and medical sciences
Female
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Microscopy, Fluorescence - standards
Middle Aged
Mycobacterium Infections - microbiology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Tuberculosis and atypical mycobacterial infections
Tuberculosis, Pulmonary - microbiology
title Detection of Mycobacterium tuberculosis complex in clinical specimens by a commercial polymerase chain reaction kit
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