Computer prediction of potential immunogenic determinants from protein amino acid sequence
In proteins, immunogenic determinants that can induce protein-reactive antipeptide anti-bodies reside mostly in those parts of the molecule that have a high tendency to form β-turns. A program for an IBM personal computer which predicts protein immunogenic determinants is described. The program pred...
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| Veröffentlicht in: | Analytical biochemistry 1987-08, Vol.165 (1), p.200-207 |
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| creator | Krchňák, Viktor Mach, Otakar Malý, Antonín |
| description | In proteins, immunogenic determinants that can induce protein-reactive antipeptide anti-bodies reside mostly in those parts of the molecule that have a high tendency to form β-turns. A program for an IBM personal computer which predicts protein immunogenic determinants is described. The program predicts potential immunogenic determinants from protein amino acid sequences according to a Chou-Fasman-based probability of a β-turn occurrence,
p > 1.5 × 10
−4 (
P. Y. Chou and G. D. Fasman, 1978,
Adv. Enzymol.
47, 46–148). Oncopeptides (whose efficacy in generating protein-reactive antipeptide antibodies has been described) with a β-turn probability of
p > 1.5 × 10
−4 elicited antipeptide antibodies that reacted with the parent oncoprotein at a rate of 96%, thus showing a surprisingly good correlation between the tendency to form a β-turn and the protein reactivity of antipeptide antibodies. Potential immunogenic determinants were predicted on myohemerythrin and myoglobin. |
| doi_str_mv | 10.1016/0003-2697(87)90220-X |
| format | Article |
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p > 1.5 × 10
−4 (
P. Y. Chou and G. D. Fasman, 1978,
Adv. Enzymol.
47, 46–148). Oncopeptides (whose efficacy in generating protein-reactive antipeptide antibodies has been described) with a β-turn probability of
p > 1.5 × 10
−4 elicited antipeptide antibodies that reacted with the parent oncoprotein at a rate of 96%, thus showing a surprisingly good correlation between the tendency to form a β-turn and the protein reactivity of antipeptide antibodies. Potential immunogenic determinants were predicted on myohemerythrin and myoglobin.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(87)90220-X</identifier><identifier>PMID: 2446525</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; antigenic determinants ; Antigenic determinants, haptens, artificial antigens ; Antigens ; Biological and medical sciences ; Computer Simulation ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hemerythrin - analogs & derivatives ; Hemerythrin - analysis ; human immunodeficiency virus ; immunogenic determinants ; Molecular immunology ; Myoglobin - analysis ; oncoproteins ; protein conformation ; Proteins - analysis ; Proteins - immunology ; synthetic peptides ; synthetic vaccines</subject><ispartof>Analytical biochemistry, 1987-08, Vol.165 (1), p.200-207</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-bb7530e558b89e121f11e92e1a7cda4c253160b52ec1bfe1bef13f9e0176d6cf3</citedby><cites>FETCH-LOGICAL-c417t-bb7530e558b89e121f11e92e1a7cda4c253160b52ec1bfe1bef13f9e0176d6cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(87)90220-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7760136$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2446525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krchňák, Viktor</creatorcontrib><creatorcontrib>Mach, Otakar</creatorcontrib><creatorcontrib>Malý, Antonín</creatorcontrib><title>Computer prediction of potential immunogenic determinants from protein amino acid sequence</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>In proteins, immunogenic determinants that can induce protein-reactive antipeptide anti-bodies reside mostly in those parts of the molecule that have a high tendency to form β-turns. A program for an IBM personal computer which predicts protein immunogenic determinants is described. The program predicts potential immunogenic determinants from protein amino acid sequences according to a Chou-Fasman-based probability of a β-turn occurrence,
p > 1.5 × 10
−4 (
P. Y. Chou and G. D. Fasman, 1978,
Adv. Enzymol.
47, 46–148). Oncopeptides (whose efficacy in generating protein-reactive antipeptide antibodies has been described) with a β-turn probability of
p > 1.5 × 10
−4 elicited antipeptide antibodies that reacted with the parent oncoprotein at a rate of 96%, thus showing a surprisingly good correlation between the tendency to form a β-turn and the protein reactivity of antipeptide antibodies. Potential immunogenic determinants were predicted on myohemerythrin and myoglobin.</description><subject>Amino Acid Sequence</subject><subject>antigenic determinants</subject><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Computer Simulation</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hemerythrin - analogs & derivatives</subject><subject>Hemerythrin - analysis</subject><subject>human immunodeficiency virus</subject><subject>immunogenic determinants</subject><subject>Molecular immunology</subject><subject>Myoglobin - analysis</subject><subject>oncoproteins</subject><subject>protein conformation</subject><subject>Proteins - analysis</subject><subject>Proteins - immunology</subject><subject>synthetic peptides</subject><subject>synthetic vaccines</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo67j6DxRyENFDa1W6k3RfFmTwCxa8KCxeQjpdkUh3Mibdgv_ejDPMUU8FVc9bVTyMPUV4jYDqDQC0jVCDftnrVwMIAc3dPbZDGFQDLQz32e6CPGSPSvkBgNhJdcWuRNcpKeSOfdun5bCtlPkh0xTcGlLkyfNDWimuwc48LMsW03eKwfGJKrmEaONauM9pqakKhsht7SZuXZh4oZ8bRUeP2QNv50JPzvWafX3_7sv-Y3P7-cOn_dvbxnWo12YctWyBpOzHfiAU6BFpEIRWu8l2TsgWFYxSkMPRE47ksfUDAWo1Kefba_bitLf-Ui-X1SyhOJpnGyltxWjdKwmq-y-InR4q3FawO4Eup1IyeXPIYbH5t0EwR_fmKNYcxZpem7_uzV2NPTvv38aFpkvoLLvOn5_ntjg7-2yjC-WCaa0AW1WxmxNGVdqvQNkUF45Cp5DJrWZK4d9__AGhKqGT</recordid><startdate>19870815</startdate><enddate>19870815</enddate><creator>Krchňák, Viktor</creator><creator>Mach, Otakar</creator><creator>Malý, Antonín</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19870815</creationdate><title>Computer prediction of potential immunogenic determinants from protein amino acid sequence</title><author>Krchňák, Viktor ; Mach, Otakar ; Malý, Antonín</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-bb7530e558b89e121f11e92e1a7cda4c253160b52ec1bfe1bef13f9e0176d6cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amino Acid Sequence</topic><topic>antigenic determinants</topic><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Computer Simulation</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hemerythrin - analogs & derivatives</topic><topic>Hemerythrin - analysis</topic><topic>human immunodeficiency virus</topic><topic>immunogenic determinants</topic><topic>Molecular immunology</topic><topic>Myoglobin - analysis</topic><topic>oncoproteins</topic><topic>protein conformation</topic><topic>Proteins - analysis</topic><topic>Proteins - immunology</topic><topic>synthetic peptides</topic><topic>synthetic vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krchňák, Viktor</creatorcontrib><creatorcontrib>Mach, Otakar</creatorcontrib><creatorcontrib>Malý, Antonín</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krchňák, Viktor</au><au>Mach, Otakar</au><au>Malý, Antonín</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Computer prediction of potential immunogenic determinants from protein amino acid sequence</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1987-08-15</date><risdate>1987</risdate><volume>165</volume><issue>1</issue><spage>200</spage><epage>207</epage><pages>200-207</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>In proteins, immunogenic determinants that can induce protein-reactive antipeptide anti-bodies reside mostly in those parts of the molecule that have a high tendency to form β-turns. A program for an IBM personal computer which predicts protein immunogenic determinants is described. The program predicts potential immunogenic determinants from protein amino acid sequences according to a Chou-Fasman-based probability of a β-turn occurrence,
p > 1.5 × 10
−4 (
P. Y. Chou and G. D. Fasman, 1978,
Adv. Enzymol.
47, 46–148). Oncopeptides (whose efficacy in generating protein-reactive antipeptide antibodies has been described) with a β-turn probability of
p > 1.5 × 10
−4 elicited antipeptide antibodies that reacted with the parent oncoprotein at a rate of 96%, thus showing a surprisingly good correlation between the tendency to form a β-turn and the protein reactivity of antipeptide antibodies. Potential immunogenic determinants were predicted on myohemerythrin and myoglobin.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2446525</pmid><doi>10.1016/0003-2697(87)90220-X</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1987-08, Vol.165 (1), p.200-207 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence antigenic determinants Antigenic determinants, haptens, artificial antigens Antigens Biological and medical sciences Computer Simulation Epitopes - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology Hemerythrin - analogs & derivatives Hemerythrin - analysis human immunodeficiency virus immunogenic determinants Molecular immunology Myoglobin - analysis oncoproteins protein conformation Proteins - analysis Proteins - immunology synthetic peptides synthetic vaccines |
| title | Computer prediction of potential immunogenic determinants from protein amino acid sequence |
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