2,5‐Di(tert‐butyl)‐1,4‐benzohydroquinone — a novel inhibitor of liver microsomal Ca2+ sequestration
Treatment of rat liver microsomes with 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone caused a dose‐related inhibition (K i⋍1 μM) of ATP‐dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ ‐stimulated ATPase activity. In contrast, the hydroquinone failed to ind...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 1987-11, Vol.224 (2), p.331-336 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 336 |
|---|---|
| container_issue | 2 |
| container_start_page | 331 |
| container_title | FEBS letters |
| container_volume | 224 |
| creator | Moore, Gregory A. McConkey, David J. Kass, Georges E.N. O'Brien, Peter J. Orrenius, Sten |
| description | Treatment of rat liver microsomes with 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone caused a dose‐related inhibition (K
i⋍1 μM) of ATP‐dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ ‐stimulated ATPase activity. In contrast, the hydroquinone failed to induce Ca2+ release from Ca2+ ‐loaded liver mitochondria (supplied with ATP), and inhibited neither the mitochondrial F1F0‐ATPase nor the Ca2+ ‐stimulated ATPase activity of the hepatic plasma membrane fraction. The inhibition of microsomal Ca2+ sequestration was not associated with any apparent alteration of membrane permeability or loss of other microsomal enzyme activities or modification of microsomal protein thiols. These findings suggest that 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone is a potent and selective inhibitor of liver microsomal Ca2+ sequestration which may be a useful tool in studies of Ca2+ fluxes in intact cells and tissues. |
| doi_str_mv | 10.1016/0014-5793(87)80479-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_77859372</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>77859372</sourcerecordid><originalsourceid>FETCH-LOGICAL-p1919-1a891701a1d416e5318de7970c3b2fc3b4c635c71aca7f9bac13d9b1e457a0173</originalsourceid><addsrcrecordid>eNo9kc9u1DAQxi0EKkvhDUDyAVWtaMATJxn7SJcuIFXiAmfLcRzVyIm3dlK0PfUROPCEfRKcdrWX-ff9NGP5I-QtsI_AoPnEGFRFjZKfCjwTrEJZyGdkBQJ5watGPCerA_KSvErpN8u9AHlEjkrZQANsRYbyvH64__vFnU42Trlq52nnz3IB59XS2vEuXO-6GG5mN4bR0of7f1TTMdxaT9147Vo3hUhDT727tZEOzsSQwqA9XevyA032ZrZpinpyYXxNXvTaJ_tmn4_Jr83lz_W34urH1-_rz1fFFiTIArSQgAw0dBU0tuYgOosSmeFt2edQmYbXBkEbjb1stQHeyRZsVaNmgPyYnDzt3S7vzufV4JKx3uvRhjkpRFFLjmUG3-3BuR1sp7bRDTru1P5_sv5-r-tktO-jHo1LBwxFPoaQsc0T9sd5uzvIwNTilFpsUIsNSqB6dEpJtbm8KBdhmQt8nEr-Hx1cjKE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77859372</pqid></control><display><type>article</type><title>2,5‐Di(tert‐butyl)‐1,4‐benzohydroquinone — a novel inhibitor of liver microsomal Ca2+ sequestration</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Moore, Gregory A. ; McConkey, David J. ; Kass, Georges E.N. ; O'Brien, Peter J. ; Orrenius, Sten</creator><creatorcontrib>Moore, Gregory A. ; McConkey, David J. ; Kass, Georges E.N. ; O'Brien, Peter J. ; Orrenius, Sten</creatorcontrib><description>Treatment of rat liver microsomes with 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone caused a dose‐related inhibition (K
i⋍1 μM) of ATP‐dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ ‐stimulated ATPase activity. In contrast, the hydroquinone failed to induce Ca2+ release from Ca2+ ‐loaded liver mitochondria (supplied with ATP), and inhibited neither the mitochondrial F1F0‐ATPase nor the Ca2+ ‐stimulated ATPase activity of the hepatic plasma membrane fraction. The inhibition of microsomal Ca2+ sequestration was not associated with any apparent alteration of membrane permeability or loss of other microsomal enzyme activities or modification of microsomal protein thiols. These findings suggest that 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone is a potent and selective inhibitor of liver microsomal Ca2+ sequestration which may be a useful tool in studies of Ca2+ fluxes in intact cells and tissues.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(87)80479-9</identifier><identifier>PMID: 2961610</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>(Ca2+ + Mg2+)-ATPase ; 2,5-Di(tert-butyl)-1,4-benzohydroquinone ; Animals ; Biological and medical sciences ; Ca2+ sequestration ; Calcium - metabolism ; Calcium-Transporting ATPases - metabolism ; Cell Compartmentation - drug effects ; Cell Membrane - enzymology ; Cytochrome P-450 Enzyme System - metabolism ; General and cellular metabolism. Vitamins ; Hydroquinones - pharmacology ; In Vitro Techniques ; Male ; Medical sciences ; Microsome ; Microsomes, Liver - drug effects ; Microsomes, Liver - metabolism ; Mitochondria, Liver - metabolism ; Pharmacology. Drug treatments ; Rat liver ; Rats</subject><ispartof>FEBS letters, 1987-11, Vol.224 (2), p.331-336</ispartof><rights>FEBS Letters 224 (1987) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7817371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2961610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moore, Gregory A.</creatorcontrib><creatorcontrib>McConkey, David J.</creatorcontrib><creatorcontrib>Kass, Georges E.N.</creatorcontrib><creatorcontrib>O'Brien, Peter J.</creatorcontrib><creatorcontrib>Orrenius, Sten</creatorcontrib><title>2,5‐Di(tert‐butyl)‐1,4‐benzohydroquinone — a novel inhibitor of liver microsomal Ca2+ sequestration</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Treatment of rat liver microsomes with 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone caused a dose‐related inhibition (K
i⋍1 μM) of ATP‐dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ ‐stimulated ATPase activity. In contrast, the hydroquinone failed to induce Ca2+ release from Ca2+ ‐loaded liver mitochondria (supplied with ATP), and inhibited neither the mitochondrial F1F0‐ATPase nor the Ca2+ ‐stimulated ATPase activity of the hepatic plasma membrane fraction. The inhibition of microsomal Ca2+ sequestration was not associated with any apparent alteration of membrane permeability or loss of other microsomal enzyme activities or modification of microsomal protein thiols. These findings suggest that 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone is a potent and selective inhibitor of liver microsomal Ca2+ sequestration which may be a useful tool in studies of Ca2+ fluxes in intact cells and tissues.</description><subject>(Ca2+ + Mg2+)-ATPase</subject><subject>2,5-Di(tert-butyl)-1,4-benzohydroquinone</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Ca2+ sequestration</subject><subject>Calcium - metabolism</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Cell Compartmentation - drug effects</subject><subject>Cell Membrane - enzymology</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>General and cellular metabolism. Vitamins</subject><subject>Hydroquinones - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microsome</subject><subject>Microsomes, Liver - drug effects</subject><subject>Microsomes, Liver - metabolism</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Rat liver</subject><subject>Rats</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc9u1DAQxi0EKkvhDUDyAVWtaMATJxn7SJcuIFXiAmfLcRzVyIm3dlK0PfUROPCEfRKcdrWX-ff9NGP5I-QtsI_AoPnEGFRFjZKfCjwTrEJZyGdkBQJ5watGPCerA_KSvErpN8u9AHlEjkrZQANsRYbyvH64__vFnU42Trlq52nnz3IB59XS2vEuXO-6GG5mN4bR0of7f1TTMdxaT9147Vo3hUhDT727tZEOzsSQwqA9XevyA032ZrZpinpyYXxNXvTaJ_tmn4_Jr83lz_W34urH1-_rz1fFFiTIArSQgAw0dBU0tuYgOosSmeFt2edQmYbXBkEbjb1stQHeyRZsVaNmgPyYnDzt3S7vzufV4JKx3uvRhjkpRFFLjmUG3-3BuR1sp7bRDTru1P5_sv5-r-tktO-jHo1LBwxFPoaQsc0T9sd5uzvIwNTilFpsUIsNSqB6dEpJtbm8KBdhmQt8nEr-Hx1cjKE</recordid><startdate>19871130</startdate><enddate>19871130</enddate><creator>Moore, Gregory A.</creator><creator>McConkey, David J.</creator><creator>Kass, Georges E.N.</creator><creator>O'Brien, Peter J.</creator><creator>Orrenius, Sten</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19871130</creationdate><title>2,5‐Di(tert‐butyl)‐1,4‐benzohydroquinone — a novel inhibitor of liver microsomal Ca2+ sequestration</title><author>Moore, Gregory A. ; McConkey, David J. ; Kass, Georges E.N. ; O'Brien, Peter J. ; Orrenius, Sten</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1919-1a891701a1d416e5318de7970c3b2fc3b4c635c71aca7f9bac13d9b1e457a0173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>(Ca2+ + Mg2+)-ATPase</topic><topic>2,5-Di(tert-butyl)-1,4-benzohydroquinone</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Ca2+ sequestration</topic><topic>Calcium - metabolism</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Cell Compartmentation - drug effects</topic><topic>Cell Membrane - enzymology</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>General and cellular metabolism. Vitamins</topic><topic>Hydroquinones - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microsome</topic><topic>Microsomes, Liver - drug effects</topic><topic>Microsomes, Liver - metabolism</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Rat liver</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moore, Gregory A.</creatorcontrib><creatorcontrib>McConkey, David J.</creatorcontrib><creatorcontrib>Kass, Georges E.N.</creatorcontrib><creatorcontrib>O'Brien, Peter J.</creatorcontrib><creatorcontrib>Orrenius, Sten</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moore, Gregory A.</au><au>McConkey, David J.</au><au>Kass, Georges E.N.</au><au>O'Brien, Peter J.</au><au>Orrenius, Sten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>2,5‐Di(tert‐butyl)‐1,4‐benzohydroquinone — a novel inhibitor of liver microsomal Ca2+ sequestration</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1987-11-30</date><risdate>1987</risdate><volume>224</volume><issue>2</issue><spage>331</spage><epage>336</epage><pages>331-336</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Treatment of rat liver microsomes with 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone caused a dose‐related inhibition (K
i⋍1 μM) of ATP‐dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ ‐stimulated ATPase activity. In contrast, the hydroquinone failed to induce Ca2+ release from Ca2+ ‐loaded liver mitochondria (supplied with ATP), and inhibited neither the mitochondrial F1F0‐ATPase nor the Ca2+ ‐stimulated ATPase activity of the hepatic plasma membrane fraction. The inhibition of microsomal Ca2+ sequestration was not associated with any apparent alteration of membrane permeability or loss of other microsomal enzyme activities or modification of microsomal protein thiols. These findings suggest that 2,5‐di(tert‐butyl)‐1,4‐benzohydroquinone is a potent and selective inhibitor of liver microsomal Ca2+ sequestration which may be a useful tool in studies of Ca2+ fluxes in intact cells and tissues.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>2961610</pmid><doi>10.1016/0014-5793(87)80479-9</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1987-11, Vol.224 (2), p.331-336 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77859372 |
| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | (Ca2+ + Mg2+)-ATPase 2,5-Di(tert-butyl)-1,4-benzohydroquinone Animals Biological and medical sciences Ca2+ sequestration Calcium - metabolism Calcium-Transporting ATPases - metabolism Cell Compartmentation - drug effects Cell Membrane - enzymology Cytochrome P-450 Enzyme System - metabolism General and cellular metabolism. Vitamins Hydroquinones - pharmacology In Vitro Techniques Male Medical sciences Microsome Microsomes, Liver - drug effects Microsomes, Liver - metabolism Mitochondria, Liver - metabolism Pharmacology. Drug treatments Rat liver Rats |
| title | 2,5‐Di(tert‐butyl)‐1,4‐benzohydroquinone — a novel inhibitor of liver microsomal Ca2+ sequestration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T03%3A39%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=2,5%E2%80%90Di(tert%E2%80%90butyl)%E2%80%901,4%E2%80%90benzohydroquinone%20%E2%80%94%20a%20novel%20inhibitor%20of%20liver%20microsomal%20Ca2+%20sequestration&rft.jtitle=FEBS%20letters&rft.au=Moore,%20Gregory%20A.&rft.date=1987-11-30&rft.volume=224&rft.issue=2&rft.spage=331&rft.epage=336&rft.pages=331-336&rft.issn=0014-5793&rft.eissn=1873-3468&rft.coden=FEBLAL&rft_id=info:doi/10.1016/0014-5793(87)80479-9&rft_dat=%3Cproquest_pubme%3E77859372%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77859372&rft_id=info:pmid/2961610&rfr_iscdi=true |