Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain)
The location of a major antigenic determinant involved in the neutralization of a flavivirus, yellow fever virus (YF), has been defined in terms of its position in the amino acid sequence of the E protein. Neutralization escape variants of the 17D vaccine strain of YF were selected with two neutrali...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1987, Vol.161 (2), p.474-478 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Lobigs, M. Dalgarno, L. Schlesinger, J.J. Weir, R.C. |
| description | The location of a major antigenic determinant involved in the neutralization of a flavivirus, yellow fever virus (YF), has been defined in terms of its position in the amino acid sequence of the E protein. Neutralization escape variants of the 17D vaccine strain of YF were selected with two neutralizing monoclonal antibodies. Nucleotide sequencing of the envelope protein genes (E and M) of the variants showed that in each variant there was a single nucleotide change in the E gene leading to a nonconservative amino acid substitution in the E protein at position 71 or 72. The changes are in a region of the E protein which is hydrophilic, rich in cysteine residues, and not conserved between flavivirus subgroups. Since the selecting monoclonal antibodies neutralize attenuated 17D and virulent Asibi strains of YF with equal efficiency (
J. J. Schlesinger, M. W. Brandriss, and T. P. Monath, 1983,
Virology 125, 8–17), it can be concluded that the neutralization determinant defined for 17D YF is also present in Asibi YF. |
| doi_str_mv | 10.1016/0042-6822(87)90141-3 |
| format | Article |
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J. J. Schlesinger, M. W. Brandriss, and T. P. Monath, 1983,
Virology 125, 8–17), it can be concluded that the neutralization determinant defined for 17D YF is also present in Asibi YF.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(87)90141-3</identifier><identifier>PMID: 2446422</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Antibodies, Monoclonal ; Antigens, Viral - analysis ; Antigens, Viral - genetics ; Arboviroses ; Biological and medical sciences ; Epitopes ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Human viral diseases ; Infectious diseases ; Medical sciences ; Microbiology ; Molecular Sequence Data ; Morphology, structure, chemical composition, physicochemical properties ; Neutralization Tests ; Tropical medicine ; Tropical viral diseases ; Viral diseases ; Viral Proteins - genetics ; Viral Proteins - immunology ; Viral Vaccines - immunology ; Virology ; Yellow fever ; yellow fever virus ; Yellow fever virus - immunology</subject><ispartof>Virology (New York, N.Y.), 1987, Vol.161 (2), p.474-478</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-f63cdf7225b7e218348ea06628db833ada282f4724a05bde7f61adf842479cf83</citedby><cites>FETCH-LOGICAL-c512t-f63cdf7225b7e218348ea06628db833ada282f4724a05bde7f61adf842479cf83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682287901413$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7588219$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2446422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lobigs, M.</creatorcontrib><creatorcontrib>Dalgarno, L.</creatorcontrib><creatorcontrib>Schlesinger, J.J.</creatorcontrib><creatorcontrib>Weir, R.C.</creatorcontrib><title>Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain)</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The location of a major antigenic determinant involved in the neutralization of a flavivirus, yellow fever virus (YF), has been defined in terms of its position in the amino acid sequence of the E protein. Neutralization escape variants of the 17D vaccine strain of YF were selected with two neutralizing monoclonal antibodies. Nucleotide sequencing of the envelope protein genes (E and M) of the variants showed that in each variant there was a single nucleotide change in the E gene leading to a nonconservative amino acid substitution in the E protein at position 71 or 72. The changes are in a region of the E protein which is hydrophilic, rich in cysteine residues, and not conserved between flavivirus subgroups. Since the selecting monoclonal antibodies neutralize attenuated 17D and virulent Asibi strains of YF with equal efficiency (
J. J. Schlesinger, M. W. Brandriss, and T. P. Monath, 1983,
Virology 125, 8–17), it can be concluded that the neutralization determinant defined for 17D YF is also present in Asibi YF.</description><subject>Amino Acid Sequence</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Viral - analysis</subject><subject>Antigens, Viral - genetics</subject><subject>Arboviroses</subject><subject>Biological and medical sciences</subject><subject>Epitopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Human viral diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Neutralization Tests</subject><subject>Tropical medicine</subject><subject>Tropical viral diseases</subject><subject>Viral diseases</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><subject>Yellow fever</subject><subject>yellow fever virus</subject><subject>Yellow fever virus - immunology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLAzEUhYMoWh__QCELEV2MJpnMJN0I4hsKbnQd0uQGI9OMJpmK_npTW7rUVbi53zkczkXokJJzSmh7QQhnVSsZO5XibEwop1W9gUaUjNuK1JxuotEa2UG7Kb2RMgtBttE247zljI2QnfRGZ98H3DuscYAhR9357-WfhQxx5oMOGfuA8yvgW_we-wz-V_AFXdd_YgdziHju45DwKRU3eK6N8QFwKmY-nO2jLae7BAerdw-93N0-Xz9Uk6f7x-urSWUaynLl2tpYJxhrpgIYlTWXoEnbMmmnsq611UwyxwXjmjRTC8K1VFsnOeNibJys99DJ0rdE_BggZTXzyZSMOkA_JCWEbEoB4l-QcimIIKSAfAma2KcUwan36Gc6filK1OIKalGxWlSspFC_V1B1kR2t_IfpDOxatKq97I9Xe52M7lzUwfi0xkQjJaPjgl0uMSilzT1ElYyHYMD6CCYr2_u_c_wAzLSiUA</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Lobigs, M.</creator><creator>Dalgarno, L.</creator><creator>Schlesinger, J.J.</creator><creator>Weir, R.C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain)</title><author>Lobigs, M. ; Dalgarno, L. ; Schlesinger, J.J. ; Weir, R.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-f63cdf7225b7e218348ea06628db833ada282f4724a05bde7f61adf842479cf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Viral - analysis</topic><topic>Antigens, Viral - genetics</topic><topic>Arboviroses</topic><topic>Biological and medical sciences</topic><topic>Epitopes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Human viral diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Neutralization Tests</topic><topic>Tropical medicine</topic><topic>Tropical viral diseases</topic><topic>Viral diseases</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><topic>Yellow fever</topic><topic>yellow fever virus</topic><topic>Yellow fever virus - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lobigs, M.</creatorcontrib><creatorcontrib>Dalgarno, L.</creatorcontrib><creatorcontrib>Schlesinger, J.J.</creatorcontrib><creatorcontrib>Weir, R.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lobigs, M.</au><au>Dalgarno, L.</au><au>Schlesinger, J.J.</au><au>Weir, R.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain)</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1987</date><risdate>1987</risdate><volume>161</volume><issue>2</issue><spage>474</spage><epage>478</epage><pages>474-478</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The location of a major antigenic determinant involved in the neutralization of a flavivirus, yellow fever virus (YF), has been defined in terms of its position in the amino acid sequence of the E protein. Neutralization escape variants of the 17D vaccine strain of YF were selected with two neutralizing monoclonal antibodies. Nucleotide sequencing of the envelope protein genes (E and M) of the variants showed that in each variant there was a single nucleotide change in the E gene leading to a nonconservative amino acid substitution in the E protein at position 71 or 72. The changes are in a region of the E protein which is hydrophilic, rich in cysteine residues, and not conserved between flavivirus subgroups. Since the selecting monoclonal antibodies neutralize attenuated 17D and virulent Asibi strains of YF with equal efficiency (
J. J. Schlesinger, M. W. Brandriss, and T. P. Monath, 1983,
Virology 125, 8–17), it can be concluded that the neutralization determinant defined for 17D YF is also present in Asibi YF.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2446422</pmid><doi>10.1016/0042-6822(87)90141-3</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Antibodies, Monoclonal Antigens, Viral - analysis Antigens, Viral - genetics Arboviroses Biological and medical sciences Epitopes Fundamental and applied biological sciences. Psychology Genes, Viral Human viral diseases Infectious diseases Medical sciences Microbiology Molecular Sequence Data Morphology, structure, chemical composition, physicochemical properties Neutralization Tests Tropical medicine Tropical viral diseases Viral diseases Viral Proteins - genetics Viral Proteins - immunology Viral Vaccines - immunology Virology Yellow fever yellow fever virus Yellow fever virus - immunology |
| title | Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain) |
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