Flow cytometry with crystal violet to detect intracytoplasmic fluorescence in viable human lymphocytes demonstration of antibody entering living cells

A new method is described for the detection of intracytoplasmic flourescence and its differentiation from surface staininng of viable human lymphocytes using flow cytometry after addition of crystal violet which quenches surface but not internal fluorescence. This has then been used to study antibod...

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Veröffentlicht in:	Journal of immunological methods 1987-11, Vol.104 (1), p.195-200
Hauptverfasser:	Ma, Jian, Chapman, Graeme V., Chen, Shun-le, Penny, Ronald, Breit, Samuel N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - immunology Antibodies - metabolism Antibody penetration of viable human lymphocytes Autoantibodies - immunology Autoantibodies - metabolism Biological and medical sciences Biological Transport Cell Membrane - immunology Cell Survival Connective Tissue Diseases - immunology Crystal violet Flow cytometry Flow Cytometry - methods Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Gentian Violet Humans Immunobiology Immunological reactions in vitro Intracytoplasmic fluorescence Leukocytes, Mononuclear - immunology Methods Ribonucleoproteins - immunology
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