A complex array of sequences enhances ribosomal transcription in Xenopus laevis
The ribosomal DNA spacer in Xenopus laevis was shown in previous studies to be involved in regulating the expression of the ribosomal genes. Here transcription enhancement by this spacer has been studied in some detail, to fully identify the sequences involved and to determine their relative importa...
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Veröffentlicht in: | Journal of molecular biology 1987-08, Vol.196 (4), p.813-827 |
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description | The ribosomal DNA spacer in
Xenopus laevis was shown in previous studies to be involved in regulating the expression of the ribosomal genes. Here transcription enhancement by this spacer has been studied in some detail, to fully identify the sequences involved and to determine their relative importance in this phenomenon. It is shown that the
60
81
base-pair (bp) repeats, which were reported to be enhancer elements, act as part of a mode of enhancement whose effect is amplified by the spacer promoters or
Bam islands. The “
Bam super repeat”, a combination of spacer promoter and
60
81
bp elements, is the major enhancer unit. Within a
Bam super repeat, a near linear correlation between the number of
60
81
bp
elements and enhancer activity is observed. Thus, there is no significant co-operativity in the binding of transcription factors to an array of these elements. Multiple
Bam super repeats do not act additively and may actually interfere with each others action. Surprisingly this effect is observed both in the presence and absence of active spacer promoters. Sequences between the 3′ end of the 28 S coding region and the first spacer promoter may also be involved in enhancement but only in a very minor fashion. In confirmation of recent studies, the presence of the unique ribosomal termination sequence, 213 bp upstream from the pre-rRNA initiation site, is essential for efficient promotion, as deletion of this sequence virtually abolishes pre-rRNA transcription. These data are discussed in terms of the possible mechanisms of transcription enhancement. |
doi_str_mv | 10.1016/0022-2836(87)90407-4 |
format | Article |
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Xenopus laevis was shown in previous studies to be involved in regulating the expression of the ribosomal genes. Here transcription enhancement by this spacer has been studied in some detail, to fully identify the sequences involved and to determine their relative importance in this phenomenon. It is shown that the
60
81
base-pair (bp) repeats, which were reported to be enhancer elements, act as part of a mode of enhancement whose effect is amplified by the spacer promoters or
Bam islands. The “
Bam super repeat”, a combination of spacer promoter and
60
81
bp elements, is the major enhancer unit. Within a
Bam super repeat, a near linear correlation between the number of
60
81
bp
elements and enhancer activity is observed. Thus, there is no significant co-operativity in the binding of transcription factors to an array of these elements. Multiple
Bam super repeats do not act additively and may actually interfere with each others action. Surprisingly this effect is observed both in the presence and absence of active spacer promoters. Sequences between the 3′ end of the 28 S coding region and the first spacer promoter may also be involved in enhancement but only in a very minor fashion. In confirmation of recent studies, the presence of the unique ribosomal termination sequence, 213 bp upstream from the pre-rRNA initiation site, is essential for efficient promotion, as deletion of this sequence virtually abolishes pre-rRNA transcription. These data are discussed in terms of the possible mechanisms of transcription enhancement.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(87)90407-4</identifier><identifier>PMID: 3681979</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; DNA, Ribosomal - genetics ; Enhancer Elements, Genetic ; Fundamental and applied biological sciences. Psychology ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation ; Promoter Regions, Genetic ; Repetitive Sequences, Nucleic Acid ; RNA Precursors - genetics ; Terminator Regions, Genetic ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing ; Xenopus laevis - genetics</subject><ispartof>Journal of molecular biology, 1987-08, Vol.196 (4), p.813-827</ispartof><rights>1987</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-d06a3921e7a903b2ec3f749ed7e08e6c1febcff1786b016fc45616387caa431d3</citedby><cites>FETCH-LOGICAL-c417t-d06a3921e7a903b2ec3f749ed7e08e6c1febcff1786b016fc45616387caa431d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-2836(87)90407-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8328520$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3681979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Winter, Ronald F.J.</creatorcontrib><creatorcontrib>Moss, Tom</creatorcontrib><title>A complex array of sequences enhances ribosomal transcription in Xenopus laevis</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The ribosomal DNA spacer in
Xenopus laevis was shown in previous studies to be involved in regulating the expression of the ribosomal genes. Here transcription enhancement by this spacer has been studied in some detail, to fully identify the sequences involved and to determine their relative importance in this phenomenon. It is shown that the
60
81
base-pair (bp) repeats, which were reported to be enhancer elements, act as part of a mode of enhancement whose effect is amplified by the spacer promoters or
Bam islands. The “
Bam super repeat”, a combination of spacer promoter and
60
81
bp elements, is the major enhancer unit. Within a
Bam super repeat, a near linear correlation between the number of
60
81
bp
elements and enhancer activity is observed. Thus, there is no significant co-operativity in the binding of transcription factors to an array of these elements. Multiple
Bam super repeats do not act additively and may actually interfere with each others action. Surprisingly this effect is observed both in the presence and absence of active spacer promoters. Sequences between the 3′ end of the 28 S coding region and the first spacer promoter may also be involved in enhancement but only in a very minor fashion. In confirmation of recent studies, the presence of the unique ribosomal termination sequence, 213 bp upstream from the pre-rRNA initiation site, is essential for efficient promotion, as deletion of this sequence virtually abolishes pre-rRNA transcription. These data are discussed in terms of the possible mechanisms of transcription enhancement.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>DNA, Ribosomal - genetics</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA Precursors - genetics</subject><subject>Terminator Regions, Genetic</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Xenopus laevis - genetics</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVo2W6S_oMUdCglObiRLK0kXwIh5AsCubTQm5DlEVWxLVfjXbr_PvbussfmJME8M7zzDCEXnH3njKtrxsqyKI1Ql0ZfVUwyXcgTsuTMVIVRwnwgyyPyiZwi_mGMrYQ0C7IQyvBKV0vyekt96oYW_lGXs9vSFCjC3zX0HpBC_9vtPjnWCVPnWjpm16PPcRhj6mns6S_o07BG2jrYRDwnH4NrET4f3jPy8-H-x91T8fL6-Hx3-1J4yfVYNEw5UZUctKuYqEvwImhZQaOBGVCeB6h9CFwbVU-7Bi9Xik87ae-cFLwRZ-Tbfu6Q05QWR9tF9NC2roe0Rqu1EVoK9i7IV6VSjPMJlHvQ54SYIdghx87lreXMzsLtbNPONq3Rdifcyqnty2H-uu6gOTYdDE_1r4e6Q-_aMOnzEY-YEaVZlXPMmz0Gk7RNhGzRx_kITczgR9uk-P8cb1PFnHg</recordid><startdate>19870820</startdate><enddate>19870820</enddate><creator>De Winter, Ronald F.J.</creator><creator>Moss, Tom</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19870820</creationdate><title>A complex array of sequences enhances ribosomal transcription in Xenopus laevis</title><author>De Winter, Ronald F.J. ; Moss, Tom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d06a3921e7a903b2ec3f749ed7e08e6c1febcff1786b016fc45616387caa431d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>DNA, Ribosomal - genetics</topic><topic>Enhancer Elements, Genetic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RNA Precursors - genetics</topic><topic>Terminator Regions, Genetic</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Xenopus laevis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Winter, Ronald F.J.</creatorcontrib><creatorcontrib>Moss, Tom</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Winter, Ronald F.J.</au><au>Moss, Tom</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A complex array of sequences enhances ribosomal transcription in Xenopus laevis</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1987-08-20</date><risdate>1987</risdate><volume>196</volume><issue>4</issue><spage>813</spage><epage>827</epage><pages>813-827</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>The ribosomal DNA spacer in
Xenopus laevis was shown in previous studies to be involved in regulating the expression of the ribosomal genes. Here transcription enhancement by this spacer has been studied in some detail, to fully identify the sequences involved and to determine their relative importance in this phenomenon. It is shown that the
60
81
base-pair (bp) repeats, which were reported to be enhancer elements, act as part of a mode of enhancement whose effect is amplified by the spacer promoters or
Bam islands. The “
Bam super repeat”, a combination of spacer promoter and
60
81
bp elements, is the major enhancer unit. Within a
Bam super repeat, a near linear correlation between the number of
60
81
bp
elements and enhancer activity is observed. Thus, there is no significant co-operativity in the binding of transcription factors to an array of these elements. Multiple
Bam super repeats do not act additively and may actually interfere with each others action. Surprisingly this effect is observed both in the presence and absence of active spacer promoters. Sequences between the 3′ end of the 28 S coding region and the first spacer promoter may also be involved in enhancement but only in a very minor fashion. In confirmation of recent studies, the presence of the unique ribosomal termination sequence, 213 bp upstream from the pre-rRNA initiation site, is essential for efficient promotion, as deletion of this sequence virtually abolishes pre-rRNA transcription. These data are discussed in terms of the possible mechanisms of transcription enhancement.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>3681979</pmid><doi>10.1016/0022-2836(87)90407-4</doi><tpages>15</tpages></addata></record> |
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subjects | Animals Biological and medical sciences DNA, Ribosomal - genetics Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid RNA Precursors - genetics Terminator Regions, Genetic Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Xenopus laevis - genetics |
title | A complex array of sequences enhances ribosomal transcription in Xenopus laevis |
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