Comparison of multiplex PCR and culture for detection of Legionellae in cooling tower water samples

We compared multiplex polymerase chain reaction (PCR) and culture for detecting the presence of Legionella pneumophila and Legionella spp in cooling tower water samples. Multiplex PCR was performed after phenol extraction of DNA from the samples. The set of primers for the PCR assay involved the 5S...

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Veröffentlicht in:	Southeast Asian journal of tropical medicine and public health 1995-06, Vol.26 (2), p.258-262
Hauptverfasser:	CHANG, H. R, LOOL. H, KUAH, B. G, HENG, B. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Air Conditioning Bacterial diseases Bacterial diseases of the respiratory system Base Sequence Biological and medical sciences DNA Primers Fluorescent Antibody Technique, Direct Human bacterial diseases Infectious diseases Legionella - genetics Legionella - isolation & purification Medical sciences Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity Singapore Tropical medicine Water Microbiology
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