Comparison of multiplex PCR and culture for detection of Legionellae in cooling tower water samples

We compared multiplex polymerase chain reaction (PCR) and culture for detecting the presence of Legionella pneumophila and Legionella spp in cooling tower water samples. Multiplex PCR was performed after phenol extraction of DNA from the samples. The set of primers for the PCR assay involved the 5S...

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Veröffentlicht in:	Southeast Asian journal of tropical medicine and public health 1995-06, Vol.26 (2), p.258-262
Hauptverfasser:	CHANG, H. R, LOOL. H, KUAH, B. G, HENG, B. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Air Conditioning Bacterial diseases Bacterial diseases of the respiratory system Base Sequence Biological and medical sciences DNA Primers Fluorescent Antibody Technique, Direct Human bacterial diseases Infectious diseases Legionella - genetics Legionella - isolation & purification Medical sciences Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity Singapore Tropical medicine Water Microbiology
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Zusammenfassung:	We compared multiplex polymerase chain reaction (PCR) and culture for detecting the presence of Legionella pneumophila and Legionella spp in cooling tower water samples. Multiplex PCR was performed after phenol extraction of DNA from the samples. The set of primers for the PCR assay involved the 5S rRNA (Legionella spp) and the mip (macrophage infectivity potentiator gene, specific for L. pneumophila) genes as target sequences for amplification. Both the sensitivity and the specificity of the PCR assay were 100% when the 5S rRNA gene was used as target sequence. Isolation of Legionellae from the samples was observed only with the PCR-positive samples. We propose that PCR be used as a screening test before attempting to culture Legionellae from cooling tower water samples.
ISSN:	0125-1562