E-Cadherin and OB-Cadherin mRNA Levels in Normal Human Colon and Colon Carcinoma
A polymerase chain reaction strategy was utilized to identify members of the cadherin family of cell adhesion molecules that are expressed in normal human colon and colon carcinoma. E-cadherin (an epithelial cadherin) and OB-cadherin (a mesenchymal cadherin) were found to be present in the colon spe...
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| Veröffentlicht in: | Experimental and molecular pathology 1995-04, Vol.62 (2), p.118-122 |
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| container_title | Experimental and molecular pathology |
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| creator | Munro, Sandra B. Turner, Jan M. Farookhi, Riaz Blaschuk, Orest W. Jothy, Serge |
| description | A polymerase chain reaction strategy was utilized to identify members of the cadherin family of cell adhesion molecules that are expressed in normal human colon and colon carcinoma. E-cadherin (an epithelial cadherin) and OB-cadherin (a mesenchymal cadherin) were found to be present in the colon specimens. Furthermore, a novel semiquantitative polymerase chain reaction (PCR) assay was developed for each of these two cadherins. These assays were used to determine the relative levels of E-cadherin and OB-cadherin in normal human colon and colon carcinoma specimens. E-cadherin levels were found to be reduced approximately twofold in the adenocarcinoma specimens in comparison to matched normal colon specimens. In contrast, OB-cadherin levels did not correlate with malignant transformation. We speculate that this novel PCR method will be widely applicable for assessing E-cadherin mRNA levels in carcinomas. |
| doi_str_mv | 10.1006/exmp.1995.1013 |
| format | Article |
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E-cadherin (an epithelial cadherin) and OB-cadherin (a mesenchymal cadherin) were found to be present in the colon specimens. Furthermore, a novel semiquantitative polymerase chain reaction (PCR) assay was developed for each of these two cadherins. These assays were used to determine the relative levels of E-cadherin and OB-cadherin in normal human colon and colon carcinoma specimens. E-cadherin levels were found to be reduced approximately twofold in the adenocarcinoma specimens in comparison to matched normal colon specimens. In contrast, OB-cadherin levels did not correlate with malignant transformation. We speculate that this novel PCR method will be widely applicable for assessing E-cadherin mRNA levels in carcinomas.</description><identifier>ISSN: 0014-4800</identifier><identifier>EISSN: 1096-0945</identifier><identifier>DOI: 10.1006/exmp.1995.1013</identifier><identifier>PMID: 8549695</identifier><identifier>CODEN: EXMPA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Adenocarcinoma - chemistry ; Base Sequence ; Biological and medical sciences ; Cadherins - analysis ; Cadherins - genetics ; Colon - chemistry ; Colonic Neoplasms - chemistry ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Medical sciences ; Molecular Sequence Data ; Polymerase Chain Reaction - methods ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Sensitivity and Specificity ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tumors</subject><ispartof>Experimental and molecular pathology, 1995-04, Vol.62 (2), p.118-122</ispartof><rights>1995 Academic Press</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-b544b8a4a9c018cda9e3cc9b397b9d235110471dd34416e214f6b2faf3b5e6653</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/exmp.1995.1013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2971027$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8549695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Munro, Sandra B.</creatorcontrib><creatorcontrib>Turner, Jan M.</creatorcontrib><creatorcontrib>Farookhi, Riaz</creatorcontrib><creatorcontrib>Blaschuk, Orest W.</creatorcontrib><creatorcontrib>Jothy, Serge</creatorcontrib><title>E-Cadherin and OB-Cadherin mRNA Levels in Normal Human Colon and Colon Carcinoma</title><title>Experimental and molecular pathology</title><addtitle>Exp Mol Pathol</addtitle><description>A polymerase chain reaction strategy was utilized to identify members of the cadherin family of cell adhesion molecules that are expressed in normal human colon and colon carcinoma. E-cadherin (an epithelial cadherin) and OB-cadherin (a mesenchymal cadherin) were found to be present in the colon specimens. Furthermore, a novel semiquantitative polymerase chain reaction (PCR) assay was developed for each of these two cadherins. These assays were used to determine the relative levels of E-cadherin and OB-cadherin in normal human colon and colon carcinoma specimens. E-cadherin levels were found to be reduced approximately twofold in the adenocarcinoma specimens in comparison to matched normal colon specimens. In contrast, OB-cadherin levels did not correlate with malignant transformation. We speculate that this novel PCR method will be widely applicable for assessing E-cadherin mRNA levels in carcinomas.</description><subject>Adenocarcinoma - chemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cadherins - analysis</subject><subject>Cadherins - genetics</subject><subject>Colon - chemistry</subject><subject>Colonic Neoplasms - chemistry</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tumors</subject><issn>0014-4800</issn><issn>1096-0945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LAzEQhoMotVav3oQ9iLetk032I8e6VCuUVkTPIZvMYmQ_atIt-u_dsqWePM28zDPD8BByTWFKAZJ7_K43UypE3EfKTsiYgkhCEDw-JWMAykOeAZyTC-8_AUAAjUZklMVcJCIek5d5mCvzgc42gWpMsH74y_XrahYscYeVD_q4al2tqmDR1aoJ8rZqh42hy5XTtmlrdUnOSlV5vDrUCXl_nL_li3C5fnrOZ8tQc8a3YRFzXmSKK6GBZtoogUxrUTCRFsJELKYUeEqNYZzTBCPKy6SISlWyIsYkidmE3A13N6796tBvZW29xqpSDbadl2maMRoB9OB0ALVrvXdYyo2ztXI_koLcK5R7hXKvUO4V9gs3h8tdUaM54gdn_fz2MFdeq6p0qtHWH7FIpBSitMeyAev14c6ik15bbDQa61BvpWntfx_8Ai_7ips</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Munro, Sandra B.</creator><creator>Turner, Jan M.</creator><creator>Farookhi, Riaz</creator><creator>Blaschuk, Orest W.</creator><creator>Jothy, Serge</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>E-Cadherin and OB-Cadherin mRNA Levels in Normal Human Colon and Colon Carcinoma</title><author>Munro, Sandra B. ; Turner, Jan M. ; Farookhi, Riaz ; Blaschuk, Orest W. ; Jothy, Serge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-b544b8a4a9c018cda9e3cc9b397b9d235110471dd34416e214f6b2faf3b5e6653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adenocarcinoma - chemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cadherins - analysis</topic><topic>Cadherins - genetics</topic><topic>Colon - chemistry</topic><topic>Colonic Neoplasms - chemistry</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Munro, Sandra B.</creatorcontrib><creatorcontrib>Turner, Jan M.</creatorcontrib><creatorcontrib>Farookhi, Riaz</creatorcontrib><creatorcontrib>Blaschuk, Orest W.</creatorcontrib><creatorcontrib>Jothy, Serge</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental and molecular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Munro, Sandra B.</au><au>Turner, Jan M.</au><au>Farookhi, Riaz</au><au>Blaschuk, Orest W.</au><au>Jothy, Serge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>E-Cadherin and OB-Cadherin mRNA Levels in Normal Human Colon and Colon Carcinoma</atitle><jtitle>Experimental and molecular pathology</jtitle><addtitle>Exp Mol Pathol</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>62</volume><issue>2</issue><spage>118</spage><epage>122</epage><pages>118-122</pages><issn>0014-4800</issn><eissn>1096-0945</eissn><coden>EXMPA6</coden><abstract>A polymerase chain reaction strategy was utilized to identify members of the cadherin family of cell adhesion molecules that are expressed in normal human colon and colon carcinoma. E-cadherin (an epithelial cadherin) and OB-cadherin (a mesenchymal cadherin) were found to be present in the colon specimens. Furthermore, a novel semiquantitative polymerase chain reaction (PCR) assay was developed for each of these two cadherins. These assays were used to determine the relative levels of E-cadherin and OB-cadherin in normal human colon and colon carcinoma specimens. E-cadherin levels were found to be reduced approximately twofold in the adenocarcinoma specimens in comparison to matched normal colon specimens. In contrast, OB-cadherin levels did not correlate with malignant transformation. We speculate that this novel PCR method will be widely applicable for assessing E-cadherin mRNA levels in carcinomas.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8549695</pmid><doi>10.1006/exmp.1995.1013</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4800 |
| ispartof | Experimental and molecular pathology, 1995-04, Vol.62 (2), p.118-122 |
| issn | 0014-4800 1096-0945 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenocarcinoma - chemistry Base Sequence Biological and medical sciences Cadherins - analysis Cadherins - genetics Colon - chemistry Colonic Neoplasms - chemistry Gastroenterology. Liver. Pancreas. Abdomen Humans Medical sciences Molecular Sequence Data Polymerase Chain Reaction - methods RNA, Messenger - analysis RNA, Messenger - genetics Sensitivity and Specificity Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
| title | E-Cadherin and OB-Cadherin mRNA Levels in Normal Human Colon and Colon Carcinoma |
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