Molecular cloning of mouse epiregulin, a novel epidermal growth factor-related protein, expressed in the early stage of development
A cDNA clone encoding a novel epidermal growth factor (EGF)-related growth regulator, epiregulin, was isolated from a cDNA library prepared from a mouse fibroblast-derived tumor cell line, NIH3T3/clone T7. The predicted amino acid sequence revealed that the purified epiregulin peptide of 46-amino ac...
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| Veröffentlicht in: | FEBS letters 1995-12, Vol.377 (3), p.403-407 |
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| creator | Toyoda, Hitoshi Komurasaki, Toshi Ikeda, Yoko Yoshimoto, Makoto Morimoto, Shigeo |
| description | A cDNA clone encoding a novel epidermal growth factor (EGF)-related growth regulator, epiregulin, was isolated from a cDNA library prepared from a mouse fibroblast-derived tumor cell line, NIH3T3/clone T7. The predicted amino acid sequence revealed that the purified epiregulin peptide of 46-amino acids was synthesized as an internal segment of a 162-amino acid putative transmembrane precursor. The structural organization was similar to that of TGF-α precursor among the members of the EGF family. Although epiregulin transcript was not detected in several adult normal tissues by Northern blot analysis, approximately 4.8-kb transcript was present in 7-day-old mouse embryo and then diminished to very low or undetectable levels. Our results suggest that epiregulin may play an important role in the regulation of epithelial cell growth during early development. |
| doi_str_mv | 10.1016/0014-5793(95)01403-9 |
| format | Article |
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The predicted amino acid sequence revealed that the purified epiregulin peptide of 46-amino acids was synthesized as an internal segment of a 162-amino acid putative transmembrane precursor. The structural organization was similar to that of TGF-α precursor among the members of the EGF family. Although epiregulin transcript was not detected in several adult normal tissues by Northern blot analysis, approximately 4.8-kb transcript was present in 7-day-old mouse embryo and then diminished to very low or undetectable levels. 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All rights reserved.</rights><rights>FEBS Letters 377 (1995) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4979-5c7da1588bed20e6b85536387058f2d479868bca99689993a11acf6b372fabbf3</citedby><cites>FETCH-LOGICAL-c4979-5c7da1588bed20e6b85536387058f2d479868bca99689993a11acf6b372fabbf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579395014039$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8549764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toyoda, Hitoshi</creatorcontrib><creatorcontrib>Komurasaki, Toshi</creatorcontrib><creatorcontrib>Ikeda, Yoko</creatorcontrib><creatorcontrib>Yoshimoto, Makoto</creatorcontrib><creatorcontrib>Morimoto, Shigeo</creatorcontrib><title>Molecular cloning of mouse epiregulin, a novel epidermal growth factor-related protein, expressed in the early stage of development</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A cDNA clone encoding a novel epidermal growth factor (EGF)-related growth regulator, epiregulin, was isolated from a cDNA library prepared from a mouse fibroblast-derived tumor cell line, NIH3T3/clone T7. 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Our results suggest that epiregulin may play an important role in the regulation of epithelial cell growth during early development.</description><subject>3T3 Cells</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>cDNA cloning</subject><subject>Cloning, Molecular</subject><subject>Development</subject><subject>DNA, Complementary - genetics</subject><subject>Embryonic and Fetal Development</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Epidermal growth factor family</subject><subject>Epiregulin</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Protein Precursors - genetics</subject><subject>Sequence Analysis</subject><subject>Tissue Distribution</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1v1DAUjBCoLIV_AJJPiEoE7Dj-ulSCqgtIRVzgbDnOy9bIiYOdtOy5fxy7u-oRONmeN2_ePE9VvST4HcGEv8eYtDUTir5R7CzfMa3Vo2pDpKA1bbl8XG0eKE-rZyn9xPktiTqpTiRrleDtprr7GjzY1ZuIrA-Tm3YoDGgMawIEs4uwW72b3iKDpnADvmA9xNF4tIvhdrlGg7FLiHUEbxbo0RzDAqUBfs8RUsqQm9ByndVM9HuUFrODMqKHLBfmEablefVkMD7Bi-N5Wv3YXn6_-Fxfffv05eLDVW2zWVUzK3pDmJQd9A0G3knGKKdSYCaHpm-Fklx21ijFpVKKGkKMHXhHRTOYrhvoafX6oJtN_lohLXp0yYL3ZoK8sBZCNpJw8U9iNsFbiguxPRBtDClFGPQc3WjiXhOsS0i6JKBLAloxfR-SVrnt1VF_7UboH5qOqeT69lC_dR72_6Wpt5cfm1IouGL3aBl0fhCC_K03DqJO1sFkoc_J2kX3wf3d6R-ZpLXm</recordid><startdate>19951227</startdate><enddate>19951227</enddate><creator>Toyoda, Hitoshi</creator><creator>Komurasaki, Toshi</creator><creator>Ikeda, Yoko</creator><creator>Yoshimoto, Makoto</creator><creator>Morimoto, Shigeo</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19951227</creationdate><title>Molecular cloning of mouse epiregulin, a novel epidermal growth factor-related protein, expressed in the early stage of development</title><author>Toyoda, Hitoshi ; 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| ispartof | FEBS letters, 1995-12, Vol.377 (3), p.403-407 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3T3 Cells Amino Acid Sequence Animals Base Sequence Blotting, Northern cDNA cloning Cloning, Molecular Development DNA, Complementary - genetics Embryonic and Fetal Development Epidermal Growth Factor - genetics Epidermal growth factor family Epiregulin Mice Molecular Sequence Data Protein Precursors - genetics Sequence Analysis Tissue Distribution |
| title | Molecular cloning of mouse epiregulin, a novel epidermal growth factor-related protein, expressed in the early stage of development |
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