Homogeneous Assays for Riboflavin Mediated by the Interaction between Enzyme–Biotin and Avidin–Riboflavin Conjugates

Homogeneous-type enzyme-linked competitive binding assays utilizing the synthetic enzyme–biotin and avidin–riboflavin conjugates are developed for the detection of riboflavin as well as its binder protein. The activity of the enzyme–biotin conjugate is inhibited in the presence of the avidin–ribofla...

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Veröffentlicht in:	Analytical biochemistry 1995-11, Vol.231 (2), p.400-406
Hauptverfasser:	Kim, Mi Jeoung, Kim, Hye Jin, Kim, Jin Mog, Kim, Bokhee, Han, Sang Hyun, Cha, Geun Sig
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Deaminase - chemistry Alkaline Phosphatase - chemistry Avidin - chemistry Carrier Proteins - analysis Catalysis Enzyme Multiplied Immunoassay Technique Feasibility Studies Glucosephosphate Dehydrogenase - chemistry Membrane Transport Proteins Riboflavin - analysis Riboflavin - chemistry
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