Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System
A trehalose-producing bacterium, Arthrobacter sp. strain Q36, was isolated from soil. From a supernatant of the culture broth, two novel enzymes related to trehalose synthesis were partially purified by Sepabeads FP-DA column chromatography. One enzyme catalyzed the conversion of maltopentaose into...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (10), p.1829-1834 |
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| container_issue | 10 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 59 |
| creator | Maruta, Kazuhiko Nakada, Tetsuya Kubota, Michio Chaen, Hiroto Sugimoto, Toshiyuki Kurimoto, Masashi Tsujisaka, Yoshio |
| description | A trehalose-producing bacterium, Arthrobacter sp. strain Q36, was isolated from soil. From a supernatant of the culture broth, two novel enzymes related to trehalose synthesis were partially purified by Sepabeads FP-DA column chromatography. One enzyme catalyzed the conversion of maltopentaose into maltotriosyl trehalose by intramolecular transglycosylation, showing it to be maltooligosyl trehalose synthase. The other hydrolyzed the product transferred by the former into maltotriose and trehalose specifically, showing it to be maltooligosyl trehalose trehalohydrolase. In addition to the bacterial strain isolated, several bacteria kept in our laboratory were found to produce these enzymes. The enzymatic system was proposed to be a novel biosynthesis of trehalose in bacteria involving the following reactions: maltodextrin→maltooligosyl trehalose, maltooligosyl trehalose→maltodextrin + trehalose. When these enzymes acted on amylose simultaneously, the trehalose in the reaction mixture reached more than 80% in content. |
| doi_str_mv | 10.1271/bbb.59.1829 |
| format | Article |
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From a supernatant of the culture broth, two novel enzymes related to trehalose synthesis were partially purified by Sepabeads FP-DA column chromatography. One enzyme catalyzed the conversion of maltopentaose into maltotriosyl trehalose by intramolecular transglycosylation, showing it to be maltooligosyl trehalose synthase. The other hydrolyzed the product transferred by the former into maltotriose and trehalose specifically, showing it to be maltooligosyl trehalose trehalohydrolase. In addition to the bacterial strain isolated, several bacteria kept in our laboratory were found to produce these enzymes. The enzymatic system was proposed to be a novel biosynthesis of trehalose in bacteria involving the following reactions: maltodextrin→maltooligosyl trehalose, maltooligosyl trehalose→maltodextrin + trehalose. When these enzymes acted on amylose simultaneously, the trehalose in the reaction mixture reached more than 80% in content.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.59.1829</identifier><identifier>PMID: 8534970</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>Amylose - metabolism ; Arthrobacter - enzymology ; Arthrobacter - isolation & purification ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Carbohydrate Sequence ; Catalysis ; Fundamental and applied biological sciences. Psychology ; Miscellaneous ; Mission oriented research ; Molecular Sequence Data ; Oligosaccharides - chemistry ; Oligosaccharides - metabolism ; Soil Microbiology ; Trehalose - biosynthesis</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1995, Vol.59 (10), p.1829-1834</ispartof><rights>Copyright 1995 Taylor and Francis Group LLC 1995</rights><rights>1996 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-912bb5cf103208ff4df1ce86390058a655e10f772e378cddca9cc55a78969e213</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2956162$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8534970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maruta, Kazuhiko</creatorcontrib><creatorcontrib>Nakada, Tetsuya</creatorcontrib><creatorcontrib>Kubota, Michio</creatorcontrib><creatorcontrib>Chaen, Hiroto</creatorcontrib><creatorcontrib>Sugimoto, Toshiyuki</creatorcontrib><creatorcontrib>Kurimoto, Masashi</creatorcontrib><creatorcontrib>Tsujisaka, Yoshio</creatorcontrib><title>Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>A trehalose-producing bacterium, Arthrobacter sp. strain Q36, was isolated from soil. From a supernatant of the culture broth, two novel enzymes related to trehalose synthesis were partially purified by Sepabeads FP-DA column chromatography. One enzyme catalyzed the conversion of maltopentaose into maltotriosyl trehalose by intramolecular transglycosylation, showing it to be maltooligosyl trehalose synthase. The other hydrolyzed the product transferred by the former into maltotriose and trehalose specifically, showing it to be maltooligosyl trehalose trehalohydrolase. In addition to the bacterial strain isolated, several bacteria kept in our laboratory were found to produce these enzymes. The enzymatic system was proposed to be a novel biosynthesis of trehalose in bacteria involving the following reactions: maltodextrin→maltooligosyl trehalose, maltooligosyl trehalose→maltodextrin + trehalose. When these enzymes acted on amylose simultaneously, the trehalose in the reaction mixture reached more than 80% in content.</description><subject>Amylose - metabolism</subject><subject>Arthrobacter - enzymology</subject><subject>Arthrobacter - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Carbohydrate Sequence</subject><subject>Catalysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Miscellaneous</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - metabolism</subject><subject>Soil Microbiology</subject><subject>Trehalose - biosynthesis</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkctr3DAQxkVpSDdJTz0HBC25FG81tp7HsORRyOPQ5CxkWUocZCuVvC3OX18tu80h5DQw8_u-Gb5B6AuQJdQCfrRtu2RqCbJWH9ACGioqrqj4iBZEAa8kZfAJHeT8REhpMNhH-5I1VAmyQNfnMQ1m6uOIo8d3yT2aELPDPsUBX5swxRj6h5iNtY8m9Z3LuJ2xwTfxjwv4bHyZN2qLf815csMR2vMmZPd5Vw_R_fnZ3eqyurq9-Lk6vaosY2yqFNRty6wH0tREek87D9ZJ3ihCmDScMQfEC1G7RkjbddYoW5RGSMWVq6E5RCdb3-cUf69dnvTQZ-tCMKOL66yFkCCkUAX8-gZ8ius0lts0UKoo4YLRQn3fUjbFnJPz-jn1g0mzBqI3EesSsWZKbyIu9PHOc90Orntld5mW-bfd3GRrgk9mtH1-xWrFOPC6YHyL9aPf_OBvTKHTk5lDTP81zXv7_wG3cZVt</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Maruta, Kazuhiko</creator><creator>Nakada, Tetsuya</creator><creator>Kubota, Michio</creator><creator>Chaen, Hiroto</creator><creator>Sugimoto, Toshiyuki</creator><creator>Kurimoto, Masashi</creator><creator>Tsujisaka, Yoshio</creator><general>Taylor & Francis</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System</title><author>Maruta, Kazuhiko ; Nakada, Tetsuya ; Kubota, Michio ; Chaen, Hiroto ; Sugimoto, Toshiyuki ; Kurimoto, Masashi ; Tsujisaka, Yoshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-912bb5cf103208ff4df1ce86390058a655e10f772e378cddca9cc55a78969e213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amylose - metabolism</topic><topic>Arthrobacter - enzymology</topic><topic>Arthrobacter - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Carbohydrate Sequence</topic><topic>Catalysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - metabolism</topic><topic>Soil Microbiology</topic><topic>Trehalose - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maruta, Kazuhiko</creatorcontrib><creatorcontrib>Nakada, Tetsuya</creatorcontrib><creatorcontrib>Kubota, Michio</creatorcontrib><creatorcontrib>Chaen, Hiroto</creatorcontrib><creatorcontrib>Sugimoto, Toshiyuki</creatorcontrib><creatorcontrib>Kurimoto, Masashi</creatorcontrib><creatorcontrib>Tsujisaka, Yoshio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maruta, Kazuhiko</au><au>Nakada, Tetsuya</au><au>Kubota, Michio</au><au>Chaen, Hiroto</au><au>Sugimoto, Toshiyuki</au><au>Kurimoto, Masashi</au><au>Tsujisaka, Yoshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1995</date><risdate>1995</risdate><volume>59</volume><issue>10</issue><spage>1829</spage><epage>1834</epage><pages>1829-1834</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>A trehalose-producing bacterium, Arthrobacter sp. strain Q36, was isolated from soil. From a supernatant of the culture broth, two novel enzymes related to trehalose synthesis were partially purified by Sepabeads FP-DA column chromatography. One enzyme catalyzed the conversion of maltopentaose into maltotriosyl trehalose by intramolecular transglycosylation, showing it to be maltooligosyl trehalose synthase. The other hydrolyzed the product transferred by the former into maltotriose and trehalose specifically, showing it to be maltooligosyl trehalose trehalohydrolase. In addition to the bacterial strain isolated, several bacteria kept in our laboratory were found to produce these enzymes. The enzymatic system was proposed to be a novel biosynthesis of trehalose in bacteria involving the following reactions: maltodextrin→maltooligosyl trehalose, maltooligosyl trehalose→maltodextrin + trehalose. When these enzymes acted on amylose simultaneously, the trehalose in the reaction mixture reached more than 80% in content.</abstract><cop>Tokyo</cop><pub>Taylor & Francis</pub><pmid>8534970</pmid><doi>10.1271/bbb.59.1829</doi><tpages>6</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1995, Vol.59 (10), p.1829-1834 |
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| source | J-STAGE Free; MEDLINE; Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Amylose - metabolism Arthrobacter - enzymology Arthrobacter - isolation & purification Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Carbohydrate Sequence Catalysis Fundamental and applied biological sciences. Psychology Miscellaneous Mission oriented research Molecular Sequence Data Oligosaccharides - chemistry Oligosaccharides - metabolism Soil Microbiology Trehalose - biosynthesis |
| title | Formation of Trehalose from Maltooligosaccharides by a Novel Enzymatic System |
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