Human eosinophils preferentially survive on tissue fibronectin compared with plasma fibronectin
Eosinophil-derived inflammatory mediators including cytokines are considered to be important in the pathogenesis of allergic inflammation. Fibronectin (Fn) has been shown to be a physiological trigger of autocrine cytokine production by human eosinophils. Fn is encoded by a single gene, but alternat...
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Veröffentlicht in: | Clinical and experimental allergy 1995-11, Vol.25 (11), p.1128-1136 |
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description | Eosinophil-derived inflammatory mediators including cytokines are considered to be important in the pathogenesis of allergic inflammation. Fibronectin (Fn) has been shown to be a physiological trigger of autocrine cytokine production by human eosinophils. Fn is encoded by a single gene, but alternate splicing of the primary RNA transcript results in polypeptide diversity in a cell type-specific fashion. Thus, tissue Fn contains approximately 50% more of the CS-1 cell binding region recognized by the integrin alpha 4 beta 1 compared with plasma Fn.
Since eosinophils are predominantly tissue-dwelling cells we compared the effect of tissue and plasma Fn on eosinophil survival in culture.
The viability and cytokine generation of eosinophils (> 99.9% pure) cultured for up to 4 days in 96 well plates coated with tissue Fn, plasma Fn or BSA was compared.
There was a significant difference in the ability of tissue Fn to support eosinophil survival compared with plasma Fn (P < 0.01). Optimal survival with tissue Fn was seen at 25 micrograms/well (70% +/- 2.0% viability at 3 days vs 7% +/- 2.2% viability on BSA). Significant (P < 0.001) cell viability on tissue Fn was observed for up to 4 days in culture (54% +/- 6.0%) compared with BSA coated wells. Addition of autologous mononuclear cells (final concentration 0.5%, 1% or 2%) resulted in plasma Fn-dependent eosinophil survival comparable to that of 99.9% pure eosinophils adherent to tissue Fn. Tissue Fn-dependent survival was significantly inhibited by anti-interleukin-3, anti-granulocyte macrophage colony stimulating factor (GM-CSF) and anti-IL-5 monoclonal antibodies. Picogram quantities of these three cytokines were detected in supernatants from eosinophils cultured for 3 days on tissue Fn using specific enzyme-linked immunosorbent assays (ELISAs). Eosinophil survival on tissue Fn was significantly inhibited by anti-beta 1 and alpha 4 beta 1 monoclonal antibody (MoAb) and also by a MoAb specific for the CS-1 motif in the IIICS region of Fn.
These observations show preferential survival of eosinophils cultured on tissue Fn as a result of alpha 4 beta 1-dependent interaction with the CS-1 region of tissue Fn triggering autocrine cytokine synthesis and release, thereby promoting their survival and persistence within the tissues. |
doi_str_mv | 10.1111/j.1365-2222.1995.tb03260.x |
format | Article |
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Since eosinophils are predominantly tissue-dwelling cells we compared the effect of tissue and plasma Fn on eosinophil survival in culture.
The viability and cytokine generation of eosinophils (> 99.9% pure) cultured for up to 4 days in 96 well plates coated with tissue Fn, plasma Fn or BSA was compared.
There was a significant difference in the ability of tissue Fn to support eosinophil survival compared with plasma Fn (P < 0.01). Optimal survival with tissue Fn was seen at 25 micrograms/well (70% +/- 2.0% viability at 3 days vs 7% +/- 2.2% viability on BSA). Significant (P < 0.001) cell viability on tissue Fn was observed for up to 4 days in culture (54% +/- 6.0%) compared with BSA coated wells. Addition of autologous mononuclear cells (final concentration 0.5%, 1% or 2%) resulted in plasma Fn-dependent eosinophil survival comparable to that of 99.9% pure eosinophils adherent to tissue Fn. Tissue Fn-dependent survival was significantly inhibited by anti-interleukin-3, anti-granulocyte macrophage colony stimulating factor (GM-CSF) and anti-IL-5 monoclonal antibodies. Picogram quantities of these three cytokines were detected in supernatants from eosinophils cultured for 3 days on tissue Fn using specific enzyme-linked immunosorbent assays (ELISAs). Eosinophil survival on tissue Fn was significantly inhibited by anti-beta 1 and alpha 4 beta 1 monoclonal antibody (MoAb) and also by a MoAb specific for the CS-1 motif in the IIICS region of Fn.
These observations show preferential survival of eosinophils cultured on tissue Fn as a result of alpha 4 beta 1-dependent interaction with the CS-1 region of tissue Fn triggering autocrine cytokine synthesis and release, thereby promoting their survival and persistence within the tissues.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/j.1365-2222.1995.tb03260.x</identifier><identifier>PMID: 8581846</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Biological and medical sciences ; Cell Survival - immunology ; Cells, Cultured ; Culture Media ; Cytokines - biosynthesis ; Eosinophils - immunology ; Eosinophils - metabolism ; Eosinophils - physiology ; Fibronectins - blood ; Fibronectins - chemistry ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunobiology ; Myeloid cells: ontogeny, maturation, markers, receptors ; Polynuclears</subject><ispartof>Clinical and experimental allergy, 1995-11, Vol.25 (11), p.1128-1136</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2907280$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8581846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WALSH, G. M</creatorcontrib><creatorcontrib>SYMON, F. A</creatorcontrib><creatorcontrib>WARDLAW, A. J</creatorcontrib><title>Human eosinophils preferentially survive on tissue fibronectin compared with plasma fibronectin</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Eosinophil-derived inflammatory mediators including cytokines are considered to be important in the pathogenesis of allergic inflammation. Fibronectin (Fn) has been shown to be a physiological trigger of autocrine cytokine production by human eosinophils. Fn is encoded by a single gene, but alternate splicing of the primary RNA transcript results in polypeptide diversity in a cell type-specific fashion. Thus, tissue Fn contains approximately 50% more of the CS-1 cell binding region recognized by the integrin alpha 4 beta 1 compared with plasma Fn.
Since eosinophils are predominantly tissue-dwelling cells we compared the effect of tissue and plasma Fn on eosinophil survival in culture.
The viability and cytokine generation of eosinophils (> 99.9% pure) cultured for up to 4 days in 96 well plates coated with tissue Fn, plasma Fn or BSA was compared.
There was a significant difference in the ability of tissue Fn to support eosinophil survival compared with plasma Fn (P < 0.01). Optimal survival with tissue Fn was seen at 25 micrograms/well (70% +/- 2.0% viability at 3 days vs 7% +/- 2.2% viability on BSA). Significant (P < 0.001) cell viability on tissue Fn was observed for up to 4 days in culture (54% +/- 6.0%) compared with BSA coated wells. Addition of autologous mononuclear cells (final concentration 0.5%, 1% or 2%) resulted in plasma Fn-dependent eosinophil survival comparable to that of 99.9% pure eosinophils adherent to tissue Fn. Tissue Fn-dependent survival was significantly inhibited by anti-interleukin-3, anti-granulocyte macrophage colony stimulating factor (GM-CSF) and anti-IL-5 monoclonal antibodies. Picogram quantities of these three cytokines were detected in supernatants from eosinophils cultured for 3 days on tissue Fn using specific enzyme-linked immunosorbent assays (ELISAs). Eosinophil survival on tissue Fn was significantly inhibited by anti-beta 1 and alpha 4 beta 1 monoclonal antibody (MoAb) and also by a MoAb specific for the CS-1 motif in the IIICS region of Fn.
These observations show preferential survival of eosinophils cultured on tissue Fn as a result of alpha 4 beta 1-dependent interaction with the CS-1 region of tissue Fn triggering autocrine cytokine synthesis and release, thereby promoting their survival and persistence within the tissues.</description><subject>Biological and medical sciences</subject><subject>Cell Survival - immunology</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Cytokines - biosynthesis</subject><subject>Eosinophils - immunology</subject><subject>Eosinophils - metabolism</subject><subject>Eosinophils - physiology</subject><subject>Fibronectins - blood</subject><subject>Fibronectins - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Myeloid cells: ontogeny, maturation, markers, receptors</subject><subject>Polynuclears</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAURoMo4zj6E4Qg4q41j7ZJljKoIwy40XW5TRMmQ_owaUfn31uwiDvP5i7O4VtchG4oSenE_T6lvMgTNpFSpfJ0qAhnBUm_TtDyV52iJVF5lgipsnN0EeOeEMJzJRdoIXNJZVYsUbkZG2ix6aJru37nfMR9MNYE0w4OvD_iOIaDOxjctXhwMY4GW1eFrjV6cC3WXdNDMDX-dMMO9x5iA3-DS3RmwUdzNd8Ven96fFtvku3r88v6YZv0VMkhkQzqurZGWy2qjCojKGOy0gWvM8GyTAFYAMg10UJwSSsma0oJ0dqCpLzmK3T3s9uH7mM0cSgbF7XxHlrTjbEUQtKCT_wXUkE4FXk-hddzOFaNqcs-uAbCsZxfN_nb2UPU4G2AVrv4mzFFBJOEfwOvzIKJ</recordid><startdate>19951101</startdate><enddate>19951101</enddate><creator>WALSH, G. M</creator><creator>SYMON, F. A</creator><creator>WARDLAW, A. J</creator><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19951101</creationdate><title>Human eosinophils preferentially survive on tissue fibronectin compared with plasma fibronectin</title><author>WALSH, G. M ; SYMON, F. A ; WARDLAW, A. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p198t-82adddfecfc7b419e71228bc63d472449aafaaa5c0c77381b28d1100ccfa813d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Biological and medical sciences</topic><topic>Cell Survival - immunology</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Cytokines - biosynthesis</topic><topic>Eosinophils - immunology</topic><topic>Eosinophils - metabolism</topic><topic>Eosinophils - physiology</topic><topic>Fibronectins - blood</topic><topic>Fibronectins - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Myeloid cells: ontogeny, maturation, markers, receptors</topic><topic>Polynuclears</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WALSH, G. M</creatorcontrib><creatorcontrib>SYMON, F. A</creatorcontrib><creatorcontrib>WARDLAW, A. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WALSH, G. M</au><au>SYMON, F. A</au><au>WARDLAW, A. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human eosinophils preferentially survive on tissue fibronectin compared with plasma fibronectin</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>1995-11-01</date><risdate>1995</risdate><volume>25</volume><issue>11</issue><spage>1128</spage><epage>1136</epage><pages>1128-1136</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Eosinophil-derived inflammatory mediators including cytokines are considered to be important in the pathogenesis of allergic inflammation. Fibronectin (Fn) has been shown to be a physiological trigger of autocrine cytokine production by human eosinophils. Fn is encoded by a single gene, but alternate splicing of the primary RNA transcript results in polypeptide diversity in a cell type-specific fashion. Thus, tissue Fn contains approximately 50% more of the CS-1 cell binding region recognized by the integrin alpha 4 beta 1 compared with plasma Fn.
Since eosinophils are predominantly tissue-dwelling cells we compared the effect of tissue and plasma Fn on eosinophil survival in culture.
The viability and cytokine generation of eosinophils (> 99.9% pure) cultured for up to 4 days in 96 well plates coated with tissue Fn, plasma Fn or BSA was compared.
There was a significant difference in the ability of tissue Fn to support eosinophil survival compared with plasma Fn (P < 0.01). Optimal survival with tissue Fn was seen at 25 micrograms/well (70% +/- 2.0% viability at 3 days vs 7% +/- 2.2% viability on BSA). Significant (P < 0.001) cell viability on tissue Fn was observed for up to 4 days in culture (54% +/- 6.0%) compared with BSA coated wells. Addition of autologous mononuclear cells (final concentration 0.5%, 1% or 2%) resulted in plasma Fn-dependent eosinophil survival comparable to that of 99.9% pure eosinophils adherent to tissue Fn. Tissue Fn-dependent survival was significantly inhibited by anti-interleukin-3, anti-granulocyte macrophage colony stimulating factor (GM-CSF) and anti-IL-5 monoclonal antibodies. Picogram quantities of these three cytokines were detected in supernatants from eosinophils cultured for 3 days on tissue Fn using specific enzyme-linked immunosorbent assays (ELISAs). Eosinophil survival on tissue Fn was significantly inhibited by anti-beta 1 and alpha 4 beta 1 monoclonal antibody (MoAb) and also by a MoAb specific for the CS-1 motif in the IIICS region of Fn.
These observations show preferential survival of eosinophils cultured on tissue Fn as a result of alpha 4 beta 1-dependent interaction with the CS-1 region of tissue Fn triggering autocrine cytokine synthesis and release, thereby promoting their survival and persistence within the tissues.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>8581846</pmid><doi>10.1111/j.1365-2222.1995.tb03260.x</doi><tpages>9</tpages></addata></record> |
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subjects | Biological and medical sciences Cell Survival - immunology Cells, Cultured Culture Media Cytokines - biosynthesis Eosinophils - immunology Eosinophils - metabolism Eosinophils - physiology Fibronectins - blood Fibronectins - chemistry Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Myeloid cells: ontogeny, maturation, markers, receptors Polynuclears |
title | Human eosinophils preferentially survive on tissue fibronectin compared with plasma fibronectin |
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