PCR strategies for isolation of the 5′ end of an immunoglobulin-encoding bovine cDNA

We have employed two in vitro amplification strategies in our attempts to characterise the variable region of bovine immunoglobulin heavy chains (V H). Products derived by 5′ RACE (rapid amplification of cDNA ends) spanned the coding sequence, but diversity in the complementarity determining regions...

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Veröffentlicht in:	Gene 1995-12, Vol.167 (1), p.285-289
Hauptverfasser:	Sinclair, M.Catherine, Aitken, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Base Sequence Cattle Cloning, Molecular - methods DNA, Complementary - genetics expression library Genes, Immunoglobulin Immunoglobulin Heavy Chains - genetics Immunoglobulin Variable Region - genetics inverse PCR Molecular Sequence Data Polymerase Chain Reaction - methods RACE variable region
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