All calbindin-immunoreactive myenteric neurons project to the mucosa of the guinea-pig small intestine
The projections of Dogiel type II myenteric neurons to the mucosa of the guinea-pig ileum were quantified by combining retrograde transport of DiI, in vitro, with immunohistochemistry. After DiI application to the mucosa over an area of 1.5 × 10 mm 2, virtually all (>97%) calbindin-immunoreactive...
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| Veröffentlicht in: | Neuroscience letters 1994-10, Vol.180 (2), p.219-222 |
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| creator | Song, Zan-Min Brookes, Simon J.H Costa, Marcello |
| description | The projections of Dogiel type II myenteric neurons to the mucosa of the guinea-pig ileum were quantified by combining retrograde transport of DiI, in vitro, with immunohistochemistry. After DiI application to the mucosa over an area of 1.5 × 10 mm
2, virtually all (>97%) calbindin-immunoreactive Dogiel type II neurons in the myenteric plexus underneath the mucosal DiI application site were labelled, indicating that essentially all of these neurons project to the mucosa. From cell counts, on average 5 calbindin-immunoreactive neurons project to each villus, and each calbindin-immunoreactive neuron supplies on average 10 villi. Since Dogiel type II neurons that were not immunoreactive for calbindin (19% of all labelled nerve cells) also projected to the mucosa, it is likely that all Dogiel type II neurons, which are putative sensory neurons of the gut, project to the mucosa. |
| doi_str_mv | 10.1016/0304-3940(94)90524-X |
| format | Article |
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2, virtually all (>97%) calbindin-immunoreactive Dogiel type II neurons in the myenteric plexus underneath the mucosal DiI application site were labelled, indicating that essentially all of these neurons project to the mucosa. From cell counts, on average 5 calbindin-immunoreactive neurons project to each villus, and each calbindin-immunoreactive neuron supplies on average 10 villi. Since Dogiel type II neurons that were not immunoreactive for calbindin (19% of all labelled nerve cells) also projected to the mucosa, it is likely that all Dogiel type II neurons, which are putative sensory neurons of the gut, project to the mucosa.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(94)90524-X</identifier><identifier>PMID: 7535407</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Axonal Transport ; Biological and medical sciences ; Calbindins ; Carbocyanines ; Cell Count ; DiI ; Enteric ; Guinea Pigs ; Intestinal Mucosa - innervation ; Intestinal Mucosa - ultrastructure ; Intestine ; Malformations of the nervous system ; Medical sciences ; Microvilli - ultrastructure ; Mucosa ; Myenteric plexus ; Myenteric Plexus - chemistry ; Myenteric Plexus - cytology ; Nerve Tissue Proteins - analysis ; Neurology ; Neurons, Afferent - chemistry ; Neurons, Afferent - ultrastructure ; Retrograde transport ; S100 Calcium Binding Protein G - analysis</subject><ispartof>Neuroscience letters, 1994-10, Vol.180 (2), p.219-222</ispartof><rights>1994</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-7742a3c1d52477b8054035189dc78ce68e0d6db6496f8d9450c490bd453a01823</citedby><cites>FETCH-LOGICAL-c483t-7742a3c1d52477b8054035189dc78ce68e0d6db6496f8d9450c490bd453a01823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-3940(94)90524-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3297571$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7535407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Zan-Min</creatorcontrib><creatorcontrib>Brookes, Simon J.H</creatorcontrib><creatorcontrib>Costa, Marcello</creatorcontrib><title>All calbindin-immunoreactive myenteric neurons project to the mucosa of the guinea-pig small intestine</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The projections of Dogiel type II myenteric neurons to the mucosa of the guinea-pig ileum were quantified by combining retrograde transport of DiI, in vitro, with immunohistochemistry. After DiI application to the mucosa over an area of 1.5 × 10 mm
2, virtually all (>97%) calbindin-immunoreactive Dogiel type II neurons in the myenteric plexus underneath the mucosal DiI application site were labelled, indicating that essentially all of these neurons project to the mucosa. From cell counts, on average 5 calbindin-immunoreactive neurons project to each villus, and each calbindin-immunoreactive neuron supplies on average 10 villi. Since Dogiel type II neurons that were not immunoreactive for calbindin (19% of all labelled nerve cells) also projected to the mucosa, it is likely that all Dogiel type II neurons, which are putative sensory neurons of the gut, project to the mucosa.</description><subject>Animals</subject><subject>Axonal Transport</subject><subject>Biological and medical sciences</subject><subject>Calbindins</subject><subject>Carbocyanines</subject><subject>Cell Count</subject><subject>DiI</subject><subject>Enteric</subject><subject>Guinea Pigs</subject><subject>Intestinal Mucosa - innervation</subject><subject>Intestinal Mucosa - ultrastructure</subject><subject>Intestine</subject><subject>Malformations of the nervous system</subject><subject>Medical sciences</subject><subject>Microvilli - ultrastructure</subject><subject>Mucosa</subject><subject>Myenteric plexus</subject><subject>Myenteric Plexus - chemistry</subject><subject>Myenteric Plexus - cytology</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Neurology</subject><subject>Neurons, Afferent - chemistry</subject><subject>Neurons, Afferent - ultrastructure</subject><subject>Retrograde transport</subject><subject>S100 Calcium Binding Protein G - analysis</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFrFTEUhYMo9Vn9BwqzENHF1JtJMkk2hVLUCoVuFLoLmeROTZlJnslMof_evL7HW9pVSO53z705h5D3FM4o0P4rMOAt0xw-a_5Fg-h4e_uCbKiSXSu17F6SzRF5Td6Ucg8Aggp-Qk6kYIKD3JDxYpoaZ6chRB9iG-Z5jSmjdUt4wGZ-xLhgDq6JuOYUS7PN6R7d0iypWf5UYHWp2CaNT7e7NUS07TbcNWW2VTjU7rLUx7fk1Wingu8O5yn5_f3br8ur9vrmx8_Li-vWccWWVkreWeaor5-RclBQl2SCKu2dVA57heB7P_Rc96PymgtwXMPguWAWqOrYKfm01617_l3rbDOH4nCabMS0FiOlorzv4VmQ9kJK6FQF-R50OZWScTTbHGabHw0Fs8vB7Ew2O5ON5uYpB3Nb2z4c9NdhRn9sOhhf6x8PdVuq_WO20YVyxFinpZC0Yud7DKtpDwGzKS5gdOhDrjEYn8L_9_gHEzOkEA</recordid><startdate>19941024</startdate><enddate>19941024</enddate><creator>Song, Zan-Min</creator><creator>Brookes, Simon J.H</creator><creator>Costa, Marcello</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19941024</creationdate><title>All calbindin-immunoreactive myenteric neurons project to the mucosa of the guinea-pig small intestine</title><author>Song, Zan-Min ; Brookes, Simon J.H ; Costa, Marcello</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-7742a3c1d52477b8054035189dc78ce68e0d6db6496f8d9450c490bd453a01823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Axonal Transport</topic><topic>Biological and medical sciences</topic><topic>Calbindins</topic><topic>Carbocyanines</topic><topic>Cell Count</topic><topic>DiI</topic><topic>Enteric</topic><topic>Guinea Pigs</topic><topic>Intestinal Mucosa - innervation</topic><topic>Intestinal Mucosa - ultrastructure</topic><topic>Intestine</topic><topic>Malformations of the nervous system</topic><topic>Medical sciences</topic><topic>Microvilli - ultrastructure</topic><topic>Mucosa</topic><topic>Myenteric plexus</topic><topic>Myenteric Plexus - chemistry</topic><topic>Myenteric Plexus - cytology</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Neurology</topic><topic>Neurons, Afferent - chemistry</topic><topic>Neurons, Afferent - ultrastructure</topic><topic>Retrograde transport</topic><topic>S100 Calcium Binding Protein G - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Zan-Min</creatorcontrib><creatorcontrib>Brookes, Simon J.H</creatorcontrib><creatorcontrib>Costa, Marcello</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Zan-Min</au><au>Brookes, Simon J.H</au><au>Costa, Marcello</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>All calbindin-immunoreactive myenteric neurons project to the mucosa of the guinea-pig small intestine</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1994-10-24</date><risdate>1994</risdate><volume>180</volume><issue>2</issue><spage>219</spage><epage>222</epage><pages>219-222</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The projections of Dogiel type II myenteric neurons to the mucosa of the guinea-pig ileum were quantified by combining retrograde transport of DiI, in vitro, with immunohistochemistry. After DiI application to the mucosa over an area of 1.5 × 10 mm
2, virtually all (>97%) calbindin-immunoreactive Dogiel type II neurons in the myenteric plexus underneath the mucosal DiI application site were labelled, indicating that essentially all of these neurons project to the mucosa. From cell counts, on average 5 calbindin-immunoreactive neurons project to each villus, and each calbindin-immunoreactive neuron supplies on average 10 villi. Since Dogiel type II neurons that were not immunoreactive for calbindin (19% of all labelled nerve cells) also projected to the mucosa, it is likely that all Dogiel type II neurons, which are putative sensory neurons of the gut, project to the mucosa.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>7535407</pmid><doi>10.1016/0304-3940(94)90524-X</doi><tpages>4</tpages></addata></record> |
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| ispartof | Neuroscience letters, 1994-10, Vol.180 (2), p.219-222 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Axonal Transport Biological and medical sciences Calbindins Carbocyanines Cell Count DiI Enteric Guinea Pigs Intestinal Mucosa - innervation Intestinal Mucosa - ultrastructure Intestine Malformations of the nervous system Medical sciences Microvilli - ultrastructure Mucosa Myenteric plexus Myenteric Plexus - chemistry Myenteric Plexus - cytology Nerve Tissue Proteins - analysis Neurology Neurons, Afferent - chemistry Neurons, Afferent - ultrastructure Retrograde transport S100 Calcium Binding Protein G - analysis |
| title | All calbindin-immunoreactive myenteric neurons project to the mucosa of the guinea-pig small intestine |
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