Glioma-stimulated chemoattraction of endothelial cells and fibroblasts in vitro: a model for the study of glioma-induced angiogenesis
Induction of angiogenesis is essential for the continued growth of solid tumors, and one critical component of tumor-induced angiogenesis involves the stimulation of microvascular cells to migrate into the growing mass. We have developed a convenient model system utilizing dual co-culture chambers t...
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| Veröffentlicht in: | Metabolic brain disease 1994-12, Vol.9 (4), p.391-399 |
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| container_title | Metabolic brain disease |
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| creator | YASSINI, P. R STICKLER, D. L BLOOMFIELD, S. M WIGGINS, R. C KONAT, G. W |
| description | Induction of angiogenesis is essential for the continued growth of solid tumors, and one critical component of tumor-induced angiogenesis involves the stimulation of microvascular cells to migrate into the growing mass. We have developed a convenient model system utilizing dual co-culture chambers to study cellular chemotaxis induced by glioma cells in vitro. In this system, rat C6 glioma cells induced migration of fibroblasts and brain capillary endothelial cells. The migratory response was directly related to the number of C6 cells serving as stimulus in the lower chamber. Similar migratory responses were induced by C6 cell conditioned medium in a concentration dependent fashion. Medium conditioned by cultured human anaplastic astrocytoma cells was also found to contain potent chemotactic factor(s). This system may ultimately be employed in the identification of particular glioma cell population(s) and secreted factor(s) responsible for the chemoattraction of microvascular cells. |
| doi_str_mv | 10.1007/BF02098885 |
| format | Article |
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Medium conditioned by cultured human anaplastic astrocytoma cells was also found to contain potent chemotactic factor(s). 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Medium conditioned by cultured human anaplastic astrocytoma cells was also found to contain potent chemotactic factor(s). This system may ultimately be employed in the identification of particular glioma cell population(s) and secreted factor(s) responsible for the chemoattraction of microvascular cells.</description><subject>Biological and medical sciences</subject><subject>Cell Movement - physiology</subject><subject>Chemotaxis - physiology</subject><subject>Culture Techniques - methods</subject><subject>Endothelium - cytology</subject><subject>Endothelium - physiology</subject><subject>Fibroblasts - physiology</subject><subject>Glioma - blood supply</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Models, Biological</subject><subject>Neovascularization, Pathologic - etiology</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Neurology</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors of the nervous system. 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The migratory response was directly related to the number of C6 cells serving as stimulus in the lower chamber. Similar migratory responses were induced by C6 cell conditioned medium in a concentration dependent fashion. Medium conditioned by cultured human anaplastic astrocytoma cells was also found to contain potent chemotactic factor(s). This system may ultimately be employed in the identification of particular glioma cell population(s) and secreted factor(s) responsible for the chemoattraction of microvascular cells.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>7534866</pmid><doi>10.1007/BF02098885</doi><tpages>9</tpages></addata></record> |
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| ispartof | Metabolic brain disease, 1994-12, Vol.9 (4), p.391-399 |
| issn | 0885-7490 1573-7365 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Biological and medical sciences Cell Movement - physiology Chemotaxis - physiology Culture Techniques - methods Endothelium - cytology Endothelium - physiology Fibroblasts - physiology Glioma - blood supply Humans Medical sciences Models, Biological Neovascularization, Pathologic - etiology Neovascularization, Pathologic - pathology Neurology Tumor Cells, Cultured Tumors of the nervous system. Phacomatoses |
| title | Glioma-stimulated chemoattraction of endothelial cells and fibroblasts in vitro: a model for the study of glioma-induced angiogenesis |
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