cDNA cloning, sequencing and in situ localization of a transcript specific to both sublingual demilune cells and parotid intercalated duct cells in mouse salivary glands

A cDNA clone derived from mouse sublingual gland was isolated from a λ-phage cDNA library. Northern blot hybridization indicated that the transcript from which it was derived was approx. 700 nucleotides in length. This mRNA encoded a protein of about 20 kDa, as determined by hybrid selection and cel...

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Veröffentlicht in:	Archives of oral biology 1994-12, Vol.39 (12), p.1011-1022
Hauptverfasser:	Bekhor, Isaac, Wen, Yi, Shi, Songtao, Hsieh, Chih-Hsin, Denny, Patricia A., Denny, Paul C.
Format:	Artikel
Sprache:	eng
Schlagworte:	5′-RACE Amino Acid Sequence Animals Base Sequence Blotting, Northern cDNA cloning Cloning, Molecular Dentistry DNA Primers DNA Restriction Enzymes DNA sequencing DNA, Complementary - isolation & purification Female Glycoproteins - biosynthesis Glycoproteins - chemistry Glycoproteins - genetics Immunohistochemistry In Situ Hybridization Male Mice Molecular Sequence Data mRNA Organ Specificity parotid Parotid Gland - cytology Parotid Gland - metabolism PCR Polymerase Chain Reaction Protein Biosynthesis RNA Probes RNA, Messenger - analysis Salivary Proteins and Peptides - biosynthesis Salivary Proteins and Peptides - chemistry Salivary Proteins and Peptides - genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid SLGp20 sublingual Sublingual Gland - cytology Sublingual Gland - metabolism
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