Selective absorption and scattering of light by solutions of macromolecules and by particulate suspensions
For particulate suspensions and for solutions that scatter light measurably the total absorbance A generally contains contributions due to specific absorption ( A a) and scattering of light ( A s). The quantity A s is closely related to the turbidity τ. In general, spectrophotometry of such systems...
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| Veröffentlicht in: | Journal of Biochemical and Biophysical Methods 1987-09, Vol.14 (6), p.303-322 |
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| container_issue | 6 |
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| container_title | Journal of Biochemical and Biophysical Methods |
| container_volume | 14 |
| creator | Heirwegh, Karel P.M. Meuwissen, Jules A.T.P. Lontie, René |
| description | For particulate suspensions and for solutions that scatter light measurably the total absorbance
A generally contains contributions due to specific absorption (
A
a) and scattering of light (
A
s). The quantity
A
s is closely related to the turbidity τ. In general, spectrophotometry of such systems requires proper modification of the spectrophotometer used in order to permit accurate determination of the absorbance
A and of the derived quantities
A
a and
A
s. Apparent deviation from Beer's law in such systems is often due to inappropriate experimental technique. After a discussion of the parameters that determine the intensity of light scattered by solutes, an account is given of the experimental precautions to be taken for determination of the absorbance of light scattering suspensions and solutions and of techniques for correcting absorbance spectra for scattering of light. Measurement of the turbidity is briefly confronted with determination of the scattering ratio
i
90°/
I
o and the impact of erroneous turbidity measurements on derived molecular parameters is disccused |
| doi_str_mv | 10.1016/0165-022X(87)90025-X |
| format | Article |
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A generally contains contributions due to specific absorption (
A
a) and scattering of light (
A
s). The quantity
A
s is closely related to the turbidity τ. In general, spectrophotometry of such systems requires proper modification of the spectrophotometer used in order to permit accurate determination of the absorbance
A and of the derived quantities
A
a and
A
s. Apparent deviation from Beer's law in such systems is often due to inappropriate experimental technique. After a discussion of the parameters that determine the intensity of light scattered by solutes, an account is given of the experimental precautions to be taken for determination of the absorbance of light scattering suspensions and solutions and of techniques for correcting absorbance spectra for scattering of light. Measurement of the turbidity is briefly confronted with determination of the scattering ratio
i
90°/
I
o and the impact of erroneous turbidity measurements on derived molecular parameters is disccused</description><identifier>ISSN: 0165-022X</identifier><identifier>EISSN: 1872-857X</identifier><identifier>DOI: 10.1016/0165-022X(87)90025-X</identifier><identifier>PMID: 3316357</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Absorption selective ; Biopolymers ; Light ; Light scattering ; Macromolecular Substances ; Particulate suspensions ; reviews ; Scattering, Radiation ; Solutions ; Solutions of macromolecules ; Spectrophotometry - methods ; turbidity</subject><ispartof>Journal of Biochemical and Biophysical Methods, 1987-09, Vol.14 (6), p.303-322</ispartof><rights>1987</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-f697021e20b320b0033b0927a8107761c08d6c9f25bb798d160a91c1830adacf3</citedby><cites>FETCH-LOGICAL-c388t-f697021e20b320b0033b0927a8107761c08d6c9f25bb798d160a91c1830adacf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>313,314,777,781,789,27903,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3316357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heirwegh, Karel P.M.</creatorcontrib><creatorcontrib>Meuwissen, Jules A.T.P.</creatorcontrib><creatorcontrib>Lontie, René</creatorcontrib><title>Selective absorption and scattering of light by solutions of macromolecules and by particulate suspensions</title><title>Journal of Biochemical and Biophysical Methods</title><addtitle>J Biochem Biophys Methods</addtitle><description>For particulate suspensions and for solutions that scatter light measurably the total absorbance
A generally contains contributions due to specific absorption (
A
a) and scattering of light (
A
s). The quantity
A
s is closely related to the turbidity τ. In general, spectrophotometry of such systems requires proper modification of the spectrophotometer used in order to permit accurate determination of the absorbance
A and of the derived quantities
A
a and
A
s. Apparent deviation from Beer's law in such systems is often due to inappropriate experimental technique. After a discussion of the parameters that determine the intensity of light scattered by solutes, an account is given of the experimental precautions to be taken for determination of the absorbance of light scattering suspensions and solutions and of techniques for correcting absorbance spectra for scattering of light. Measurement of the turbidity is briefly confronted with determination of the scattering ratio
i
90°/
I
o and the impact of erroneous turbidity measurements on derived molecular parameters is disccused</description><subject>Absorption selective</subject><subject>Biopolymers</subject><subject>Light</subject><subject>Light scattering</subject><subject>Macromolecular Substances</subject><subject>Particulate suspensions</subject><subject>reviews</subject><subject>Scattering, Radiation</subject><subject>Solutions</subject><subject>Solutions of macromolecules</subject><subject>Spectrophotometry - methods</subject><subject>turbidity</subject><issn>0165-022X</issn><issn>1872-857X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVpSDbbfoMGfCrNwYn-xJZ8KZTQJoFADklhb0KWx1sttuVq5IV8-8i7yx6TwyCY-b0n6Q0h3xi9YpSV16mKnHK--qHkZUUpL_LVJ7JgSvJcFXL1mSyOyBk5R9xQSoXiN6fkVAhWikIuyOYZOrDRbSEzNfowRueHzAxNhtbECMEN68y3WefW_2JWv2bou2lmcO72xgbf--QwdYA7WUJGE6JLHRMhwwlHGHAWfCEnrekQvh7OJfn75_fL7X3--HT3cPvrMbdCqZi3ZSUpZ8BpLVKlJ4uaVlwaxaiUJbNUNaWtWl7UtaxUw0pqKmaZEtQ0xrZiSb7vfcfg_0-AUfcOLXSdGcBPqKVMToLJD0FWcF5UskjgzR5Mv0UM0OoxuN6EV82onneh56D1HLRWUu92oVdJdnHwn-oemqPoEH6a_9zPIaWxdRA0WgeDhcaFtBPdePf-BW9n65pw</recordid><startdate>19870901</startdate><enddate>19870901</enddate><creator>Heirwegh, Karel P.M.</creator><creator>Meuwissen, Jules A.T.P.</creator><creator>Lontie, René</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19870901</creationdate><title>Selective absorption and scattering of light by solutions of macromolecules and by particulate suspensions</title><author>Heirwegh, Karel P.M. ; Meuwissen, Jules A.T.P. ; Lontie, René</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-f697021e20b320b0033b0927a8107761c08d6c9f25bb798d160a91c1830adacf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Absorption selective</topic><topic>Biopolymers</topic><topic>Light</topic><topic>Light scattering</topic><topic>Macromolecular Substances</topic><topic>Particulate suspensions</topic><topic>reviews</topic><topic>Scattering, Radiation</topic><topic>Solutions</topic><topic>Solutions of macromolecules</topic><topic>Spectrophotometry - methods</topic><topic>turbidity</topic><toplevel>online_resources</toplevel><creatorcontrib>Heirwegh, Karel P.M.</creatorcontrib><creatorcontrib>Meuwissen, Jules A.T.P.</creatorcontrib><creatorcontrib>Lontie, René</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Biochemical and Biophysical Methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heirwegh, Karel P.M.</au><au>Meuwissen, Jules A.T.P.</au><au>Lontie, René</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective absorption and scattering of light by solutions of macromolecules and by particulate suspensions</atitle><jtitle>Journal of Biochemical and Biophysical Methods</jtitle><addtitle>J Biochem Biophys Methods</addtitle><date>1987-09-01</date><risdate>1987</risdate><volume>14</volume><issue>6</issue><spage>303</spage><epage>322</epage><pages>303-322</pages><issn>0165-022X</issn><eissn>1872-857X</eissn><abstract>For particulate suspensions and for solutions that scatter light measurably the total absorbance
A generally contains contributions due to specific absorption (
A
a) and scattering of light (
A
s). The quantity
A
s is closely related to the turbidity τ. In general, spectrophotometry of such systems requires proper modification of the spectrophotometer used in order to permit accurate determination of the absorbance
A and of the derived quantities
A
a and
A
s. Apparent deviation from Beer's law in such systems is often due to inappropriate experimental technique. After a discussion of the parameters that determine the intensity of light scattered by solutes, an account is given of the experimental precautions to be taken for determination of the absorbance of light scattering suspensions and solutions and of techniques for correcting absorbance spectra for scattering of light. Measurement of the turbidity is briefly confronted with determination of the scattering ratio
i
90°/
I
o and the impact of erroneous turbidity measurements on derived molecular parameters is disccused</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>3316357</pmid><doi>10.1016/0165-022X(87)90025-X</doi><tpages>20</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-022X |
| ispartof | Journal of Biochemical and Biophysical Methods, 1987-09, Vol.14 (6), p.303-322 |
| issn | 0165-022X 1872-857X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77810317 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Absorption selective Biopolymers Light Light scattering Macromolecular Substances Particulate suspensions reviews Scattering, Radiation Solutions Solutions of macromolecules Spectrophotometry - methods turbidity |
| title | Selective absorption and scattering of light by solutions of macromolecules and by particulate suspensions |
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