Direct sequencing of terminal regions of genomic P1 clones: A general strategy for the design of sequence-tagged site markers

A method for the preparation of P1 DNA is presented, which allows the direct sequencing of ends of inserts in genomic P1 clones using the Applied Biosystems 373A DNA Sequencer and the Dye Terminator sequencing methodology. We surveyed several common methods of DNA preparation including alkaline lysi...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Genetic analysis, techniques and applications techniques and applications, 1994, Vol.11 (5), p.117-128
Hauptverfasser:	Kimmerly, William J., Kyle, Ami L., Lustre, Veronica M., Martin, Christopher H., J. Palazzolo, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriophage P1 - genetics Base Sequence Chromosome Mapping Cloning, Molecular - methods Conjugation, Genetic Drosophila melanogaster - genetics Escherichia coli - classification Escherichia coli - genetics Gene Library Genetic Vectors - genetics Molecular Sequence Data Sequence Tagged Sites Templates, Genetic
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!