Identification of a 20-kDa protein with calcium uptake transport activity. Reconstitution in a membrane model
This paper presents results of experiments designed to further purify the membrane system involved in mitochondrial calcium transport. A partially purified extract, which transported calcium with a specific activity of 1194 nmol 45Ca2+/mg protein/5 min, was used to obtain mouse hyperimmune serum. Th...
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| Veröffentlicht in: | Journal of bioenergetics and biomembranes 1994-10, Vol.26 (5), p.555-562 |
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| container_issue | 5 |
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| container_title | Journal of bioenergetics and biomembranes |
| container_volume | 26 |
| creator | Zazueta, C Massò, F Paez, A Bravo, C Vega, A Montaño, L Vázquez, M Ramírez, J Chávez, E |
| description | This paper presents results of experiments designed to further purify the membrane system involved in mitochondrial calcium transport. A partially purified extract, which transported calcium with a specific activity of 1194 nmol 45Ca2+/mg protein/5 min, was used to obtain mouse hyperimmune serum. This serum inhibited calcium uptake both in mitoplasts and in vesicles reconstituted with mitochondrial proteins containing cytochrome oxidase. Western blot analysis of the semipurified fraction showed that the serum recognized specifically two antigens of 75 and 20 kDa. Both antibodies were purified by elution from the nitrocellulose sheets and their inhibition capacity was analyzed. The antibody that recognized the 20-kDa protein produced a higher degree of inhibition than the other one. |
| doi_str_mv | 10.1007/BF00762740 |
| format | Article |
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Both antibodies were purified by elution from the nitrocellulose sheets and their inhibition capacity was analyzed. 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| ispartof | Journal of bioenergetics and biomembranes, 1994-10, Vol.26 (5), p.555-562 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological Transport Blotting, Western Calcium - metabolism Carrier Proteins - isolation & purification Carrier Proteins - metabolism Electrophoresis, Polyacrylamide Gel Intracellular Membranes - metabolism Kidney Cortex - metabolism Kinetics Membrane Proteins - isolation & purification Membrane Proteins - metabolism Mitochondria - metabolism Models, Biological Molecular Weight Rats |
| title | Identification of a 20-kDa protein with calcium uptake transport activity. Reconstitution in a membrane model |
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