N-3 and n-6 fatty acid metabolism in undifferentiated and differentiated human intestine cell line (Caco-2)
Metabolism of n-6 and n-3 fatty acids in the undifferentiated and differentiated human adenocarcinoma colon cell line (Caco-2) was studied. In cells incubated with either 18:2n-6 or 18:3n-3, no significant amounts of long chain n-6 and n-3 metabolites were found. Incubation with either 18:3n-6 or 18...
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Veröffentlicht in: | Molecular and cellular biochemistry 1995-10, Vol.151 (2), p.121-130 |
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description | Metabolism of n-6 and n-3 fatty acids in the undifferentiated and differentiated human adenocarcinoma colon cell line (Caco-2) was studied. In cells incubated with either 18:2n-6 or 18:3n-3, no significant amounts of long chain n-6 and n-3 metabolites were found. Incubation with either 18:3n-6 or 18:4n-3 raised significantly the levels of 20:3n-6 and 20:4n-3, respectively. In the undifferentiated cells, significant proportions of 20:3n-6 and 20:4n-3 were further delta 5-desaturated to form 20:4n-6 and 20:5n-3, respectively. Incubation with either 20:4n-6 or 20:5n-3 raised the levels of their direct elongation products, 22:4n-6 and 22:5n-3, respectively. Incubation with 22:4n-6 or 22:5n-3 increased the levels of 20:4n-6 and 20:5n-6. These results suggest that delta 6-desaturation in the Caco-2 cells is less active in comparison with elongation, delta 5-desaturation and retro-conversion. These enzymes were modulated by the state of differentiation, and appeared to be non-specific to n-3 and n-6 fatty acids. When cells were incubated with 18:3n-6 and 18:4n-3 concomitantly, the levels of incorporation of total n-6 fatty acids into cellular lipids were greater than those of the n-3 fatty acids, whereas the ratios of 20+22 carbon metabolites to 18-carbon precursor favored n-3 over n-6 fatty acids. These results suggest that n-3 and n-6 fatty acids were not metabolized identically in Caco-2 cells. |
doi_str_mv | 10.1007/bf01322334 |
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In cells incubated with either 18:2n-6 or 18:3n-3, no significant amounts of long chain n-6 and n-3 metabolites were found. Incubation with either 18:3n-6 or 18:4n-3 raised significantly the levels of 20:3n-6 and 20:4n-3, respectively. In the undifferentiated cells, significant proportions of 20:3n-6 and 20:4n-3 were further delta 5-desaturated to form 20:4n-6 and 20:5n-3, respectively. Incubation with either 20:4n-6 or 20:5n-3 raised the levels of their direct elongation products, 22:4n-6 and 22:5n-3, respectively. Incubation with 22:4n-6 or 22:5n-3 increased the levels of 20:4n-6 and 20:5n-6. These results suggest that delta 6-desaturation in the Caco-2 cells is less active in comparison with elongation, delta 5-desaturation and retro-conversion. These enzymes were modulated by the state of differentiation, and appeared to be non-specific to n-3 and n-6 fatty acids. When cells were incubated with 18:3n-6 and 18:4n-3 concomitantly, the levels of incorporation of total n-6 fatty acids into cellular lipids were greater than those of the n-3 fatty acids, whereas the ratios of 20+22 carbon metabolites to 18-carbon precursor favored n-3 over n-6 fatty acids. These results suggest that n-3 and n-6 fatty acids were not metabolized identically in Caco-2 cells.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/bf01322334</identifier><identifier>PMID: 8569757</identifier><language>eng</language><publisher>Netherlands</publisher><subject>alpha-Linolenic Acid - metabolism ; Caco-2 Cells ; Cell Differentiation ; cell lines ; Epithelial Cells ; Epithelium - metabolism ; fatty acid desaturation ; fatty acid elongation ; Fatty Acids, Omega-3 - metabolism ; Fatty Acids, Omega-6 ; Fatty Acids, Unsaturated - metabolism ; human cell lines ; Humans ; Intestine, Small - cytology ; Intestine, Small - metabolism ; intestines ; Linoleic Acid ; Linoleic Acids - metabolism ; lipid metabolism ; omega-3 fatty acids ; omega-6 fatty acids ; Phospholipids - metabolism ; polyunsaturated fatty acids ; Triglycerides - metabolism</subject><ispartof>Molecular and cellular biochemistry, 1995-10, Vol.151 (2), p.121-130</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-2f8c820496d2f798b6e2e45c5b353be1504a44525d06badea4d8e8fae41b3b863</citedby><cites>FETCH-LOGICAL-c372t-2f8c820496d2f798b6e2e45c5b353be1504a44525d06badea4d8e8fae41b3b863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8569757$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Y.S</creatorcontrib><creatorcontrib>Liu, J.W</creatorcontrib><creatorcontrib>Koba, K</creatorcontrib><creatorcontrib>Anderson, S.N</creatorcontrib><title>N-3 and n-6 fatty acid metabolism in undifferentiated and differentiated human intestine cell line (Caco-2)</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Metabolism of n-6 and n-3 fatty acids in the undifferentiated and differentiated human adenocarcinoma colon cell line (Caco-2) was studied. In cells incubated with either 18:2n-6 or 18:3n-3, no significant amounts of long chain n-6 and n-3 metabolites were found. Incubation with either 18:3n-6 or 18:4n-3 raised significantly the levels of 20:3n-6 and 20:4n-3, respectively. In the undifferentiated cells, significant proportions of 20:3n-6 and 20:4n-3 were further delta 5-desaturated to form 20:4n-6 and 20:5n-3, respectively. Incubation with either 20:4n-6 or 20:5n-3 raised the levels of their direct elongation products, 22:4n-6 and 22:5n-3, respectively. Incubation with 22:4n-6 or 22:5n-3 increased the levels of 20:4n-6 and 20:5n-6. These results suggest that delta 6-desaturation in the Caco-2 cells is less active in comparison with elongation, delta 5-desaturation and retro-conversion. These enzymes were modulated by the state of differentiation, and appeared to be non-specific to n-3 and n-6 fatty acids. When cells were incubated with 18:3n-6 and 18:4n-3 concomitantly, the levels of incorporation of total n-6 fatty acids into cellular lipids were greater than those of the n-3 fatty acids, whereas the ratios of 20+22 carbon metabolites to 18-carbon precursor favored n-3 over n-6 fatty acids. These results suggest that n-3 and n-6 fatty acids were not metabolized identically in Caco-2 cells.</description><subject>alpha-Linolenic Acid - metabolism</subject><subject>Caco-2 Cells</subject><subject>Cell Differentiation</subject><subject>cell lines</subject><subject>Epithelial Cells</subject><subject>Epithelium - metabolism</subject><subject>fatty acid desaturation</subject><subject>fatty acid elongation</subject><subject>Fatty Acids, Omega-3 - metabolism</subject><subject>Fatty Acids, Omega-6</subject><subject>Fatty Acids, Unsaturated - metabolism</subject><subject>human cell lines</subject><subject>Humans</subject><subject>Intestine, Small - cytology</subject><subject>Intestine, Small - metabolism</subject><subject>intestines</subject><subject>Linoleic Acid</subject><subject>Linoleic Acids - metabolism</subject><subject>lipid metabolism</subject><subject>omega-3 fatty acids</subject><subject>omega-6 fatty acids</subject><subject>Phospholipids - metabolism</subject><subject>polyunsaturated fatty acids</subject><subject>Triglycerides - metabolism</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkDtPxDAQhC0EOo6Dhh7hCgFSwM_YKeHEAdIJCrg6suM1GPKAOCnu35N7QEG1q51vVqNB6JiSK0qIuraeUM4Y52IHjalUPBEZzXbRmHBCEk2V2kcHMX4QMuCUjtBIyzRTUo3R51PCsakdrpMUe9N1S2yK4HAFnbFNGWKFQ4372gXvoYW6C6YDt3b8O733lakHuIPYhRpwAWWJy9V2PjVFk7CLQ7TnTRnhaDsnaDG7e50-JPPn-8fpzTwpuGJdwrwuNCMiSx3zKtM2BQZCFtJyyS1QSYQRQjLpSGqNAyOcBu0NCGq51SmfoLPN36-2-e6HOHkV4iqOqaHpY66UJqlcg5cbsGibGFvw-VcbKtMuc0ryVbP57ey32QE-2X7tbQXuD91WOeinG92bJjdvbYj54oUNdkIlpUwp_gN5NHuU</recordid><startdate>19951018</startdate><enddate>19951018</enddate><creator>Huang, Y.S</creator><creator>Liu, J.W</creator><creator>Koba, K</creator><creator>Anderson, S.N</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951018</creationdate><title>N-3 and n-6 fatty acid metabolism in undifferentiated and differentiated human intestine cell line (Caco-2)</title><author>Huang, Y.S ; Liu, J.W ; Koba, K ; Anderson, S.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-2f8c820496d2f798b6e2e45c5b353be1504a44525d06badea4d8e8fae41b3b863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>alpha-Linolenic Acid - metabolism</topic><topic>Caco-2 Cells</topic><topic>Cell Differentiation</topic><topic>cell lines</topic><topic>Epithelial Cells</topic><topic>Epithelium - metabolism</topic><topic>fatty acid desaturation</topic><topic>fatty acid elongation</topic><topic>Fatty Acids, Omega-3 - metabolism</topic><topic>Fatty Acids, Omega-6</topic><topic>Fatty Acids, Unsaturated - metabolism</topic><topic>human cell lines</topic><topic>Humans</topic><topic>Intestine, Small - cytology</topic><topic>Intestine, Small - metabolism</topic><topic>intestines</topic><topic>Linoleic Acid</topic><topic>Linoleic Acids - metabolism</topic><topic>lipid metabolism</topic><topic>omega-3 fatty acids</topic><topic>omega-6 fatty acids</topic><topic>Phospholipids - metabolism</topic><topic>polyunsaturated fatty acids</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Y.S</creatorcontrib><creatorcontrib>Liu, J.W</creatorcontrib><creatorcontrib>Koba, K</creatorcontrib><creatorcontrib>Anderson, S.N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Y.S</au><au>Liu, J.W</au><au>Koba, K</au><au>Anderson, S.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-3 and n-6 fatty acid metabolism in undifferentiated and differentiated human intestine cell line (Caco-2)</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1995-10-18</date><risdate>1995</risdate><volume>151</volume><issue>2</issue><spage>121</spage><epage>130</epage><pages>121-130</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Metabolism of n-6 and n-3 fatty acids in the undifferentiated and differentiated human adenocarcinoma colon cell line (Caco-2) was studied. In cells incubated with either 18:2n-6 or 18:3n-3, no significant amounts of long chain n-6 and n-3 metabolites were found. Incubation with either 18:3n-6 or 18:4n-3 raised significantly the levels of 20:3n-6 and 20:4n-3, respectively. In the undifferentiated cells, significant proportions of 20:3n-6 and 20:4n-3 were further delta 5-desaturated to form 20:4n-6 and 20:5n-3, respectively. Incubation with either 20:4n-6 or 20:5n-3 raised the levels of their direct elongation products, 22:4n-6 and 22:5n-3, respectively. Incubation with 22:4n-6 or 22:5n-3 increased the levels of 20:4n-6 and 20:5n-6. These results suggest that delta 6-desaturation in the Caco-2 cells is less active in comparison with elongation, delta 5-desaturation and retro-conversion. These enzymes were modulated by the state of differentiation, and appeared to be non-specific to n-3 and n-6 fatty acids. When cells were incubated with 18:3n-6 and 18:4n-3 concomitantly, the levels of incorporation of total n-6 fatty acids into cellular lipids were greater than those of the n-3 fatty acids, whereas the ratios of 20+22 carbon metabolites to 18-carbon precursor favored n-3 over n-6 fatty acids. These results suggest that n-3 and n-6 fatty acids were not metabolized identically in Caco-2 cells.</abstract><cop>Netherlands</cop><pmid>8569757</pmid><doi>10.1007/bf01322334</doi><tpages>10</tpages></addata></record> |
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subjects | alpha-Linolenic Acid - metabolism Caco-2 Cells Cell Differentiation cell lines Epithelial Cells Epithelium - metabolism fatty acid desaturation fatty acid elongation Fatty Acids, Omega-3 - metabolism Fatty Acids, Omega-6 Fatty Acids, Unsaturated - metabolism human cell lines Humans Intestine, Small - cytology Intestine, Small - metabolism intestines Linoleic Acid Linoleic Acids - metabolism lipid metabolism omega-3 fatty acids omega-6 fatty acids Phospholipids - metabolism polyunsaturated fatty acids Triglycerides - metabolism |
title | N-3 and n-6 fatty acid metabolism in undifferentiated and differentiated human intestine cell line (Caco-2) |
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