Considerations in the development of a sensitive HPLC assay for human epidermal growth factors in human plasma

A sensitive assay was developed for human epidermal growth factors (hEGF) 1–48 (dosed), hEGF 1–53 (endogenous), without interference from potential metabolites hEGFs 1–47 or 1–46. Spiked human plasma samples were injected directly, utilizing on-line immunoaffinity HPLC (anti-hEGF) clean-up. No chang...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 1995-09, Vol.13 (10), p.1205-1213
Hauptverfasser:	Kagel, John R., Rossi, David T., Nordblom, Gerald D., Dudeck, Richard C., Barksdale, Charles M., Kuo, Be-Sheng, Wright, D.Scott
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analysis Animals Biological and medical sciences Chromatography, Affinity Chromatography, High Pressure Liquid Direct plasma injection Enzyme-Linked Immunosorbent Assay Epidermal growth factor Epidermal Growth Factor - blood Female General pharmacology Humans Medical sciences Molecular Sequence Data Pharmacology. Drug treatments Rabbits Tandem immunoaffinity and SCX HPLC Trace quantitation
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