Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth
Glandular epithelial cells (GEC) and stromal cells (SC) were isolated from guinea-pig endometrium and cultured separately. After the cells were made quiescent by serum depletion, cell proliferation and c-fos gene expression were investigated. Estradiol-17 beta (E2) alone had no effect on cell prolif...
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| Veröffentlicht in: | Comptes rendus des seances de la Societe de biologie et de ses filiales 1994, Vol.188 (4), p.387-396 |
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| container_title | Comptes rendus des seances de la Societe de biologie et de ses filiales |
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| creator | Ordener, C Lascombe, I Sallot, M Cypriani, B Adessi, G L Jouvenot, M |
| description | Glandular epithelial cells (GEC) and stromal cells (SC) were isolated from guinea-pig endometrium and cultured separately. After the cells were made quiescent by serum depletion, cell proliferation and c-fos gene expression were investigated. Estradiol-17 beta (E2) alone had no effect on cell proliferation or c-fos gene expression. Cell proliferation was observed in SC and GEC after stimulation with insulin plus EGF and a supplementary effect was obtained when E2 was associated with these factors only in GEC. In GEC, insulin plus EGF had no effect on c-fos expression but an effect was observed when E2 was associated with these factors or with a protein synthesis inhibitor, cycloheximide (Chx) or puromycine. An E2 effect in the presence of Chx was also observed in SC. These data suggest the presence of a labile protein preventing the in vitro estrogenic action in endometrial cells. |
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After the cells were made quiescent by serum depletion, cell proliferation and c-fos gene expression were investigated. Estradiol-17 beta (E2) alone had no effect on cell proliferation or c-fos gene expression. Cell proliferation was observed in SC and GEC after stimulation with insulin plus EGF and a supplementary effect was obtained when E2 was associated with these factors only in GEC. In GEC, insulin plus EGF had no effect on c-fos expression but an effect was observed when E2 was associated with these factors or with a protein synthesis inhibitor, cycloheximide (Chx) or puromycine. An E2 effect in the presence of Chx was also observed in SC. These data suggest the presence of a labile protein preventing the in vitro estrogenic action in endometrial cells.</description><identifier>ISSN: 0037-9026</identifier><identifier>PMID: 7736261</identifier><language>fre</language><publisher>France</publisher><subject>Animals ; Cell Division - drug effects ; Cells, Cultured ; Endometrium - cytology ; Epidermal Growth Factor - pharmacology ; Estradiol - pharmacology ; Female ; Gene Expression - drug effects ; Genes, fos - genetics ; Guinea Pigs ; In Vitro Techniques ; Insulin-Like Growth Factor I - pharmacology ; Stromal Cells - cytology</subject><ispartof>Comptes rendus des seances de la Societe de biologie et de ses filiales, 1994, Vol.188 (4), p.387-396</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7736261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ordener, C</creatorcontrib><creatorcontrib>Lascombe, I</creatorcontrib><creatorcontrib>Sallot, M</creatorcontrib><creatorcontrib>Cypriani, B</creatorcontrib><creatorcontrib>Adessi, G L</creatorcontrib><creatorcontrib>Jouvenot, M</creatorcontrib><title>Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth</title><title>Comptes rendus des seances de la Societe de biologie et de ses filiales</title><addtitle>C R Seances Soc Biol Fil</addtitle><description>Glandular epithelial cells (GEC) and stromal cells (SC) were isolated from guinea-pig endometrium and cultured separately. After the cells were made quiescent by serum depletion, cell proliferation and c-fos gene expression were investigated. Estradiol-17 beta (E2) alone had no effect on cell proliferation or c-fos gene expression. Cell proliferation was observed in SC and GEC after stimulation with insulin plus EGF and a supplementary effect was obtained when E2 was associated with these factors only in GEC. In GEC, insulin plus EGF had no effect on c-fos expression but an effect was observed when E2 was associated with these factors or with a protein synthesis inhibitor, cycloheximide (Chx) or puromycine. An E2 effect in the presence of Chx was also observed in SC. These data suggest the presence of a labile protein preventing the in vitro estrogenic action in endometrial cells.</description><subject>Animals</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Endometrium - cytology</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Gene Expression - drug effects</subject><subject>Genes, fos - genetics</subject><subject>Guinea Pigs</subject><subject>In Vitro Techniques</subject><subject>Insulin-Like Growth Factor I - pharmacology</subject><subject>Stromal Cells - cytology</subject><issn>0037-9026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotUMlqwzAU1KElDWk-oaBTTzVosaXoWEK6QCCX3I1sPaUCW3Ilpctf9JOrLJe3DMPMm3eD5oRwWSnCxB1apuQ6QgilipBmhmZScsEEnaO_jbXQ54SDxVTiDrKuIOWojQsD1t7gQwzf-QNb3ecQC8_jHoYBTzEMzkLU2RXoROwrGxI-gAcMP1OE4hn8E3Yef7kcw3kCb8IIOTo9nGXSCfQhjmW_GN2jW6uHBMtrX6D9y2a_fqu2u9f39fO2mhpOq1o3pJaWUNOxWgLhbEUMA0ZsozSTomaqo6orZSUkNVIIDgCqt7xXsuGGL9DjRbbk-DyWxO3o0uki7SEcUyvlilDVyEJ8uBKP3QimnaIbdfxtrx_k_yRfbbw</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Ordener, C</creator><creator>Lascombe, I</creator><creator>Sallot, M</creator><creator>Cypriani, B</creator><creator>Adessi, G L</creator><creator>Jouvenot, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth</title><author>Ordener, C ; Lascombe, I ; Sallot, M ; Cypriani, B ; Adessi, G L ; Jouvenot, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p531-4a5047f01db247e03280d2e20f59a276429b19b9b18671d7663eee9cf3c9753d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>fre</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Endometrium - cytology</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Gene Expression - drug effects</topic><topic>Genes, fos - genetics</topic><topic>Guinea Pigs</topic><topic>In Vitro Techniques</topic><topic>Insulin-Like Growth Factor I - pharmacology</topic><topic>Stromal Cells - cytology</topic><toplevel>online_resources</toplevel><creatorcontrib>Ordener, C</creatorcontrib><creatorcontrib>Lascombe, I</creatorcontrib><creatorcontrib>Sallot, M</creatorcontrib><creatorcontrib>Cypriani, B</creatorcontrib><creatorcontrib>Adessi, G L</creatorcontrib><creatorcontrib>Jouvenot, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Comptes rendus des seances de la Societe de biologie et de ses filiales</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ordener, C</au><au>Lascombe, I</au><au>Sallot, M</au><au>Cypriani, B</au><au>Adessi, G L</au><au>Jouvenot, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth</atitle><jtitle>Comptes rendus des seances de la Societe de biologie et de ses filiales</jtitle><addtitle>C R Seances Soc Biol Fil</addtitle><date>1994</date><risdate>1994</risdate><volume>188</volume><issue>4</issue><spage>387</spage><epage>396</epage><pages>387-396</pages><issn>0037-9026</issn><abstract>Glandular epithelial cells (GEC) and stromal cells (SC) were isolated from guinea-pig endometrium and cultured separately. 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| identifier | ISSN: 0037-9026 |
| ispartof | Comptes rendus des seances de la Societe de biologie et de ses filiales, 1994, Vol.188 (4), p.387-396 |
| issn | 0037-9026 |
| language | fre |
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| source | MEDLINE; Gallica Periodicals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Cell Division - drug effects Cells, Cultured Endometrium - cytology Epidermal Growth Factor - pharmacology Estradiol - pharmacology Female Gene Expression - drug effects Genes, fos - genetics Guinea Pigs In Vitro Techniques Insulin-Like Growth Factor I - pharmacology Stromal Cells - cytology |
| title | Effects of 17 beta-estradiol and growth factors on cell proliferation and c-fos gene expression, in vitro, in endometrial cells in normal growth |
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