Large increase in thermal stability of the CH2 domain of rabbit IgG after acid treatment as evidenced by differential scanning calorimetry

Rabbit IgG after exposure to 0.05 M glycine-HCl, pH 2.0, and native IgG were compared by diffential scanning calorimetry (DSC) at pH 3.5 and C1q binding studies at pH 7.8. For acid-treated IgG, a large increase (by approx. 12–15°C) in thermal stability of the CH2 domain occurs and this domain no lon...

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