Chicken interferon-mediated induction of major histocompatibility complex Class II antigens on peripheral blood monocytes
Conditioned medium containing immune interferon (IFN) activity was prepared by stimulating spleen lymphocytes obtained from inbred SC chickens with 10 μg concanavalin A (Con A) for 48 h. Pretreatment of spleen cells with monoclonal antibody against CD4, but not CD8, abrogated IFN production suggesti...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 1994-12, Vol.44 (1), p.71-84 |
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| creator | Kaspers, B. Lillehoj, H.S. Jenkins, M.C. Pharr, G.T. |
| description | Conditioned medium containing immune interferon (IFN) activity was prepared by stimulating spleen lymphocytes obtained from inbred SC chickens with 10 μg concanavalin A (Con A) for 48 h. Pretreatment of spleen cells with monoclonal antibody against CD4, but not CD8, abrogated IFN production suggesting that CD4+ lymphocytes are responsible for immune IFN production. Immune IFN was purified 25-fold from Con A conditioned medium using controlled-pore glass column chromatography resulting in an increase in specific antiviral activity from 7 to 3290 units mg
−1. Partially purified immune IFN retained antiviral and macrophage-activating factor (MAF)-like activities. Normal peripheral blood macrophages, when cultured in the presence of partially purified immune IFN, showed a dose-dependent increase in cell surface major histocompatibility complex Class II antigen expression by flow cytometry. Northern blot analysis of mRNA obtained from IFN-treated macrophages showed a concomitant increase in Class II gene expression. This effect was more obvious in cells induced for 48 h than in those induced for 24 h. These results strongly suggest the existence of an avian homologue of the MAF-like activity. |
| doi_str_mv | 10.1016/0165-2427(94)90170-8 |
| format | Article |
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−1. Partially purified immune IFN retained antiviral and macrophage-activating factor (MAF)-like activities. Normal peripheral blood macrophages, when cultured in the presence of partially purified immune IFN, showed a dose-dependent increase in cell surface major histocompatibility complex Class II antigen expression by flow cytometry. Northern blot analysis of mRNA obtained from IFN-treated macrophages showed a concomitant increase in Class II gene expression. This effect was more obvious in cells induced for 48 h than in those induced for 24 h. These results strongly suggest the existence of an avian homologue of the MAF-like activity.</description><subject>Animals</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Blotting, Northern - veterinary</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>CHICKENS</subject><subject>Chickens - immunology</subject><subject>Concanavalin A</subject><subject>Culture Media, Conditioned</subject><subject>Dose-Response Relationship, Drug</subject><subject>Genes, MHC Class II</subject><subject>Histocompatibility Antigens Class II - biosynthesis</subject><subject>INTERFERON</subject><subject>INTERFERONAS</subject><subject>INTERFERONS</subject><subject>Interferons - biosynthesis</subject><subject>Interferons - isolation & purification</subject><subject>Interferons - pharmacology</subject><subject>Lymphocyte Activation - physiology</subject><subject>Macrophage-Activating Factors - biosynthesis</subject><subject>MAJOR HISTOCOMPATIBILITY COMPLEX</subject><subject>MONOCITOS</subject><subject>MONOCYTE</subject><subject>MONOCYTES</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - metabolism</subject><subject>POLLO</subject><subject>POULET</subject><subject>RNA, Messenger - biosynthesis</subject><subject>SISTEMA MHC</subject><subject>Spleen - cytology</subject><subject>SYSTEME MHC</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEUhYnRjO3oCxhNWBldlEJVUcBmEtPxp5OObnRNKLhMM1ZBCbSx317K7sxSF4TAOefe5DsIvaDkLSV0eFcPa9q-5a9l_0YSykkjHqANFbxrWtb1D9Hm3vIYPcn5jhDCpBBX6IqzbpBi2KDT9uDNDwjYhwLJQYqhmcF6XcDWP3s0xceAo8OzvosJH3wu0cR50cWPfvLlhNfXBL_xdtI5490O61D8LYSMa3CB5JcDJD3hcYrR4jmGaE4F8lP0yOkpw7PLfY2-f_zwbfu52X_9tNu-3zem511pRtla1ltLuADKBQVmR3CMUytJKzrTaSooMwPRzgjetqOgwnAupeuZdYR21-jVee6S4s8j5KJmnw1Mkw4Qj1nxahaUyP8aaSXGBWPV2J-NJsWcEzi1JD_rdFKUqLUatXJXK3cle_W3GiVq7OVl_nGsiO9Dly6q_vysOx2Vvk0-qy97OZC10CrenEWoqH55SCobD8HUqhKYomz0_97-BzDlp7I</recordid><startdate>19941201</startdate><enddate>19941201</enddate><creator>Kaspers, B.</creator><creator>Lillehoj, H.S.</creator><creator>Jenkins, M.C.</creator><creator>Pharr, G.T.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19941201</creationdate><title>Chicken interferon-mediated induction of major histocompatibility complex Class II antigens on peripheral blood monocytes</title><author>Kaspers, B. ; Lillehoj, H.S. ; Jenkins, M.C. ; Pharr, G.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-b92d54dd078e1781e5dbef571d90283c3a1815c60afc8722b818c7799f45df013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Blotting, Northern - veterinary</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>CHICKENS</topic><topic>Chickens - immunology</topic><topic>Concanavalin A</topic><topic>Culture Media, Conditioned</topic><topic>Dose-Response Relationship, Drug</topic><topic>Genes, MHC Class II</topic><topic>Histocompatibility Antigens Class II - biosynthesis</topic><topic>INTERFERON</topic><topic>INTERFERONAS</topic><topic>INTERFERONS</topic><topic>Interferons - biosynthesis</topic><topic>Interferons - isolation & purification</topic><topic>Interferons - pharmacology</topic><topic>Lymphocyte Activation - physiology</topic><topic>Macrophage-Activating Factors - biosynthesis</topic><topic>MAJOR HISTOCOMPATIBILITY COMPLEX</topic><topic>MONOCITOS</topic><topic>MONOCYTE</topic><topic>MONOCYTES</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - metabolism</topic><topic>POLLO</topic><topic>POULET</topic><topic>RNA, Messenger - biosynthesis</topic><topic>SISTEMA MHC</topic><topic>Spleen - cytology</topic><topic>SYSTEME MHC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaspers, B.</creatorcontrib><creatorcontrib>Lillehoj, H.S.</creatorcontrib><creatorcontrib>Jenkins, M.C.</creatorcontrib><creatorcontrib>Pharr, G.T.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaspers, B.</au><au>Lillehoj, H.S.</au><au>Jenkins, M.C.</au><au>Pharr, G.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chicken interferon-mediated induction of major histocompatibility complex Class II antigens on peripheral blood monocytes</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1994-12-01</date><risdate>1994</risdate><volume>44</volume><issue>1</issue><spage>71</spage><epage>84</epage><pages>71-84</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Conditioned medium containing immune interferon (IFN) activity was prepared by stimulating spleen lymphocytes obtained from inbred SC chickens with 10 μg concanavalin A (Con A) for 48 h. Pretreatment of spleen cells with monoclonal antibody against CD4, but not CD8, abrogated IFN production suggesting that CD4+ lymphocytes are responsible for immune IFN production. Immune IFN was purified 25-fold from Con A conditioned medium using controlled-pore glass column chromatography resulting in an increase in specific antiviral activity from 7 to 3290 units mg
−1. Partially purified immune IFN retained antiviral and macrophage-activating factor (MAF)-like activities. Normal peripheral blood macrophages, when cultured in the presence of partially purified immune IFN, showed a dose-dependent increase in cell surface major histocompatibility complex Class II antigen expression by flow cytometry. Northern blot analysis of mRNA obtained from IFN-treated macrophages showed a concomitant increase in Class II gene expression. This effect was more obvious in cells induced for 48 h than in those induced for 24 h. These results strongly suggest the existence of an avian homologue of the MAF-like activity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7536986</pmid><doi>10.1016/0165-2427(94)90170-8</doi><tpages>14</tpages></addata></record> |
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| subjects | Animals ANTIGENE ANTIGENOS ANTIGENS Blotting, Northern - veterinary CD4-Positive T-Lymphocytes - metabolism Cells, Cultured Chick Embryo CHICKENS Chickens - immunology Concanavalin A Culture Media, Conditioned Dose-Response Relationship, Drug Genes, MHC Class II Histocompatibility Antigens Class II - biosynthesis INTERFERON INTERFERONAS INTERFERONS Interferons - biosynthesis Interferons - isolation & purification Interferons - pharmacology Lymphocyte Activation - physiology Macrophage-Activating Factors - biosynthesis MAJOR HISTOCOMPATIBILITY COMPLEX MONOCITOS MONOCYTE MONOCYTES Monocytes - drug effects Monocytes - metabolism POLLO POULET RNA, Messenger - biosynthesis SISTEMA MHC Spleen - cytology SYSTEME MHC |
| title | Chicken interferon-mediated induction of major histocompatibility complex Class II antigens on peripheral blood monocytes |
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