Thrombin triggers the de novo expression of an inducible NO synthase in porcine aortic valve endothelial cells

The nitric oxide (NO) production by porcine aortic valve endothelial cells was estimated in cusps incubated at 37°C by measuring their cyclic GMP content and the nitrite levels of the incubation medium. After a stabilization period, incubations for 5 min with acetylcholine, bradykinin, ADP and bovin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:European journal of pharmacology 1995-10, Vol.291 (2), p.67-72
Hauptverfasser: De Meyer, Erwin, Van Hove, Cor E., Feng, Xiao Jie, Rampart, Marc, Herman, Arnold G.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 72
container_issue 2
container_start_page 67
container_title European journal of pharmacology
container_volume 291
creator De Meyer, Erwin
Van Hove, Cor E.
Feng, Xiao Jie
Rampart, Marc
Herman, Arnold G.
description The nitric oxide (NO) production by porcine aortic valve endothelial cells was estimated in cusps incubated at 37°C by measuring their cyclic GMP content and the nitrite levels of the incubation medium. After a stabilization period, incubations for 5 min with acetylcholine, bradykinin, ADP and bovine thrombin resulted in a receptor-mediated increase in cyclic GMP which could be blocked by EGTA, N-ω- nitro- L- arginine methyl ester (L-NAME) and N G-monomethyl- L-arginine (L-NMMA). Incubation with lipopolysaccharide (endotoxin) from E. coli O111:B4 or bovine thrombin for 5 h, dose-dependently increased nitrite production as well as cyclic GMP content. The elevated nitrite production was completely abolished in the presence of the protein synthesis inhibitor cycloheximide, was reduced by more than 50% by dexamethasone but was not affected by EGTA. L-NMMA dose-dependently reduced the increased nitrite production and cyclic GMP content. These results suggest that besides the presence of a constitutive NO synthase in porcine aortic valve endothelial cells thrombin, like lipopolysaccharide, triggers the de novo expression of an inducible Ca 2+-independent NO synthase.
doi_str_mv 10.1016/0922-4106(95)90126-4
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77795420</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0922410695901264</els_id><sourcerecordid>77795420</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-f64d22e5a591e0e7f6efd685f7b7462000838ff2b1608e86d11d1c773c64d773</originalsourceid><addsrcrecordid>eNp9kE9rGzEQxXVoSdM03yABHUJpD9tKslZaXQoh9B-E5uK70EqjWGEtOZq1ab595Nr42NPAm_ceMz9Crjj7whlXX5kRopOcqU-m_2wYF6qTb8j5SX5H3iM-McYMN_KMnA29Ulzrc5KXq1rWY8p0runxESrSeQU0AM1lVyj83VRATCXTEqnLNOWw9WmcgP55oPiS55VDaCrdlOpTBupKnZOnOzftgEIOpdVNyU3UwzThB_I2ugnh8jgvyPLH9-Xdr-7-4efvu9v7zi8GNXdRySAE9K43HBjoqCAGNfRRj1oq0f4YFkOMYuSKDTCowHngXuuFb8E2LsjHQ-2mluct4GzXCfcHuAxli1ZrbXopWDPKg9HXglgh2k1Na1dfLGd2T9buEdo9Qmt6-4-slS12fezfjmsIp9ARa9vfHPcOvZtiddknPNmE4UIOotm-HWzQUOwSVIs-QfYQUgU_21DS_-94BRRrl2I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77795420</pqid></control><display><type>article</type><title>Thrombin triggers the de novo expression of an inducible NO synthase in porcine aortic valve endothelial cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Alma/SFX Local Collection</source><creator>De Meyer, Erwin ; Van Hove, Cor E. ; Feng, Xiao Jie ; Rampart, Marc ; Herman, Arnold G.</creator><creatorcontrib>De Meyer, Erwin ; Van Hove, Cor E. ; Feng, Xiao Jie ; Rampart, Marc ; Herman, Arnold G.</creatorcontrib><description>The nitric oxide (NO) production by porcine aortic valve endothelial cells was estimated in cusps incubated at 37°C by measuring their cyclic GMP content and the nitrite levels of the incubation medium. After a stabilization period, incubations for 5 min with acetylcholine, bradykinin, ADP and bovine thrombin resulted in a receptor-mediated increase in cyclic GMP which could be blocked by EGTA, N-ω- nitro- L- arginine methyl ester (L-NAME) and N G-monomethyl- L-arginine (L-NMMA). Incubation with lipopolysaccharide (endotoxin) from E. coli O111:B4 or bovine thrombin for 5 h, dose-dependently increased nitrite production as well as cyclic GMP content. The elevated nitrite production was completely abolished in the presence of the protein synthesis inhibitor cycloheximide, was reduced by more than 50% by dexamethasone but was not affected by EGTA. L-NMMA dose-dependently reduced the increased nitrite production and cyclic GMP content. These results suggest that besides the presence of a constitutive NO synthase in porcine aortic valve endothelial cells thrombin, like lipopolysaccharide, triggers the de novo expression of an inducible Ca 2+-independent NO synthase.</description><identifier>ISSN: 0922-4106</identifier><identifier>ISSN: 0014-2999</identifier><identifier>DOI: 10.1016/0922-4106(95)90126-4</identifier><identifier>PMID: 8566177</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Aorta - drug effects ; Aorta - enzymology ; Aortic valve cusp ; Arginine - analogs &amp; derivatives ; Arginine - pharmacology ; Biological and medical sciences ; Blood vessels and receptors ; Cyclic GMP ; Cyclic GMP - metabolism ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - enzymology ; Enzyme Inhibitors - pharmacology ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; NG-Nitroarginine Methyl Ester ; Nitric oxide (NO) ; Nitric Oxide Synthase - biosynthesis ; Nitric Oxide Synthase - metabolism ; Nitric oxide synthase, constitutive ; Nitric oxide synthase, inducible ; porcine ; Swine ; Thrombin ; Thrombin - pharmacology ; Vertebrates: cardiovascular system</subject><ispartof>European journal of pharmacology, 1995-10, Vol.291 (2), p.67-72</ispartof><rights>1995</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-f64d22e5a591e0e7f6efd685f7b7462000838ff2b1608e86d11d1c773c64d773</citedby><cites>FETCH-LOGICAL-c386t-f64d22e5a591e0e7f6efd685f7b7462000838ff2b1608e86d11d1c773c64d773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2912482$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8566177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Meyer, Erwin</creatorcontrib><creatorcontrib>Van Hove, Cor E.</creatorcontrib><creatorcontrib>Feng, Xiao Jie</creatorcontrib><creatorcontrib>Rampart, Marc</creatorcontrib><creatorcontrib>Herman, Arnold G.</creatorcontrib><title>Thrombin triggers the de novo expression of an inducible NO synthase in porcine aortic valve endothelial cells</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The nitric oxide (NO) production by porcine aortic valve endothelial cells was estimated in cusps incubated at 37°C by measuring their cyclic GMP content and the nitrite levels of the incubation medium. After a stabilization period, incubations for 5 min with acetylcholine, bradykinin, ADP and bovine thrombin resulted in a receptor-mediated increase in cyclic GMP which could be blocked by EGTA, N-ω- nitro- L- arginine methyl ester (L-NAME) and N G-monomethyl- L-arginine (L-NMMA). Incubation with lipopolysaccharide (endotoxin) from E. coli O111:B4 or bovine thrombin for 5 h, dose-dependently increased nitrite production as well as cyclic GMP content. The elevated nitrite production was completely abolished in the presence of the protein synthesis inhibitor cycloheximide, was reduced by more than 50% by dexamethasone but was not affected by EGTA. L-NMMA dose-dependently reduced the increased nitrite production and cyclic GMP content. These results suggest that besides the presence of a constitutive NO synthase in porcine aortic valve endothelial cells thrombin, like lipopolysaccharide, triggers the de novo expression of an inducible Ca 2+-independent NO synthase.</description><subject>Animals</subject><subject>Aorta - drug effects</subject><subject>Aorta - enzymology</subject><subject>Aortic valve cusp</subject><subject>Arginine - analogs &amp; derivatives</subject><subject>Arginine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>Cyclic GMP</subject><subject>Cyclic GMP - metabolism</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - enzymology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>NG-Nitroarginine Methyl Ester</subject><subject>Nitric oxide (NO)</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Nitric oxide synthase, constitutive</subject><subject>Nitric oxide synthase, inducible</subject><subject>porcine</subject><subject>Swine</subject><subject>Thrombin</subject><subject>Thrombin - pharmacology</subject><subject>Vertebrates: cardiovascular system</subject><issn>0922-4106</issn><issn>0014-2999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9rGzEQxXVoSdM03yABHUJpD9tKslZaXQoh9B-E5uK70EqjWGEtOZq1ab595Nr42NPAm_ceMz9Crjj7whlXX5kRopOcqU-m_2wYF6qTb8j5SX5H3iM-McYMN_KMnA29Ulzrc5KXq1rWY8p0runxESrSeQU0AM1lVyj83VRATCXTEqnLNOWw9WmcgP55oPiS55VDaCrdlOpTBupKnZOnOzftgEIOpdVNyU3UwzThB_I2ugnh8jgvyPLH9-Xdr-7-4efvu9v7zi8GNXdRySAE9K43HBjoqCAGNfRRj1oq0f4YFkOMYuSKDTCowHngXuuFb8E2LsjHQ-2mluct4GzXCfcHuAxli1ZrbXopWDPKg9HXglgh2k1Na1dfLGd2T9buEdo9Qmt6-4-slS12fezfjmsIp9ARa9vfHPcOvZtiddknPNmE4UIOotm-HWzQUOwSVIs-QfYQUgU_21DS_-94BRRrl2I</recordid><startdate>19951015</startdate><enddate>19951015</enddate><creator>De Meyer, Erwin</creator><creator>Van Hove, Cor E.</creator><creator>Feng, Xiao Jie</creator><creator>Rampart, Marc</creator><creator>Herman, Arnold G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951015</creationdate><title>Thrombin triggers the de novo expression of an inducible NO synthase in porcine aortic valve endothelial cells</title><author>De Meyer, Erwin ; Van Hove, Cor E. ; Feng, Xiao Jie ; Rampart, Marc ; Herman, Arnold G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-f64d22e5a591e0e7f6efd685f7b7462000838ff2b1608e86d11d1c773c64d773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Aorta - drug effects</topic><topic>Aorta - enzymology</topic><topic>Aortic valve cusp</topic><topic>Arginine - analogs &amp; derivatives</topic><topic>Arginine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blood vessels and receptors</topic><topic>Cyclic GMP</topic><topic>Cyclic GMP - metabolism</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - enzymology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>NG-Nitroarginine Methyl Ester</topic><topic>Nitric oxide (NO)</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Nitric oxide synthase, constitutive</topic><topic>Nitric oxide synthase, inducible</topic><topic>porcine</topic><topic>Swine</topic><topic>Thrombin</topic><topic>Thrombin - pharmacology</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Meyer, Erwin</creatorcontrib><creatorcontrib>Van Hove, Cor E.</creatorcontrib><creatorcontrib>Feng, Xiao Jie</creatorcontrib><creatorcontrib>Rampart, Marc</creatorcontrib><creatorcontrib>Herman, Arnold G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Meyer, Erwin</au><au>Van Hove, Cor E.</au><au>Feng, Xiao Jie</au><au>Rampart, Marc</au><au>Herman, Arnold G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thrombin triggers the de novo expression of an inducible NO synthase in porcine aortic valve endothelial cells</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1995-10-15</date><risdate>1995</risdate><volume>291</volume><issue>2</issue><spage>67</spage><epage>72</epage><pages>67-72</pages><issn>0922-4106</issn><issn>0014-2999</issn><abstract>The nitric oxide (NO) production by porcine aortic valve endothelial cells was estimated in cusps incubated at 37°C by measuring their cyclic GMP content and the nitrite levels of the incubation medium. After a stabilization period, incubations for 5 min with acetylcholine, bradykinin, ADP and bovine thrombin resulted in a receptor-mediated increase in cyclic GMP which could be blocked by EGTA, N-ω- nitro- L- arginine methyl ester (L-NAME) and N G-monomethyl- L-arginine (L-NMMA). Incubation with lipopolysaccharide (endotoxin) from E. coli O111:B4 or bovine thrombin for 5 h, dose-dependently increased nitrite production as well as cyclic GMP content. The elevated nitrite production was completely abolished in the presence of the protein synthesis inhibitor cycloheximide, was reduced by more than 50% by dexamethasone but was not affected by EGTA. L-NMMA dose-dependently reduced the increased nitrite production and cyclic GMP content. These results suggest that besides the presence of a constitutive NO synthase in porcine aortic valve endothelial cells thrombin, like lipopolysaccharide, triggers the de novo expression of an inducible Ca 2+-independent NO synthase.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8566177</pmid><doi>10.1016/0922-4106(95)90126-4</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0922-4106
ispartof European journal of pharmacology, 1995-10, Vol.291 (2), p.67-72
issn 0922-4106
0014-2999
language eng
recordid cdi_proquest_miscellaneous_77795420
source MEDLINE; Elsevier ScienceDirect Journals; Alma/SFX Local Collection
subjects Animals
Aorta - drug effects
Aorta - enzymology
Aortic valve cusp
Arginine - analogs & derivatives
Arginine - pharmacology
Biological and medical sciences
Blood vessels and receptors
Cyclic GMP
Cyclic GMP - metabolism
Endothelium, Vascular - drug effects
Endothelium, Vascular - enzymology
Enzyme Inhibitors - pharmacology
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
NG-Nitroarginine Methyl Ester
Nitric oxide (NO)
Nitric Oxide Synthase - biosynthesis
Nitric Oxide Synthase - metabolism
Nitric oxide synthase, constitutive
Nitric oxide synthase, inducible
porcine
Swine
Thrombin
Thrombin - pharmacology
Vertebrates: cardiovascular system
title Thrombin triggers the de novo expression of an inducible NO synthase in porcine aortic valve endothelial cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T16%3A07%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Thrombin%20triggers%20the%20de%20novo%20expression%20of%20an%20inducible%20NO%20synthase%20in%20porcine%20aortic%20valve%20endothelial%20cells&rft.jtitle=European%20journal%20of%20pharmacology&rft.au=De%20Meyer,%20Erwin&rft.date=1995-10-15&rft.volume=291&rft.issue=2&rft.spage=67&rft.epage=72&rft.pages=67-72&rft.issn=0922-4106&rft_id=info:doi/10.1016/0922-4106(95)90126-4&rft_dat=%3Cproquest_cross%3E77795420%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77795420&rft_id=info:pmid/8566177&rft_els_id=0922410695901264&rfr_iscdi=true