Ultrastructural immunolocalization of GAP-43 in the sprouted mossy fibres of kainic acid lesioned rats
KAINIC acid (KA)-induced seizures in adult rats have been reported to cause sprouting of mossy fibres in the inner molecular layer (IML) of the dentate gyrus. In the present study, using electron microscopic immunostaining for GAP-43 we found that 3 months after KA treatment, several GAP-43 positive...
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| Veröffentlicht in: | Neuroreport 1994-12, Vol.5 (18), p.2645-2648 |
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| creator | Bendotti, Caterina Baldessari, Sonia Samanin, Rosario Biasi, Silvia De |
| description | KAINIC acid (KA)-induced seizures in adult rats have been reported to cause sprouting of mossy fibres in the inner molecular layer (IML) of the dentate gyrus. In the present study, using electron microscopic immunostaining for GAP-43 we found that 3 months after KA treatment, several GAP-43 positive terminals in the IML showed structural characteristics of mossy fibre boutons. No GAP-43-positive mossy fibre terminals were found in the normal projection areas of granule cells, thus indicating that newly synthesized GAP-43 is transported predominantly in the axonal branches actively undergoing remodelling. These results provide evidence of the involvement of GAP-43 in the structural remodelling of mossy fibres, and suggest a role of this protein in the functional activity of the sprouted mossy fibres as a consequence of KA-induced seizures. |
| doi_str_mv | 10.1097/00001756-199412000-00060 |
| format | Article |
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In the present study, using electron microscopic immunostaining for GAP-43 we found that 3 months after KA treatment, several GAP-43 positive terminals in the IML showed structural characteristics of mossy fibre boutons. No GAP-43-positive mossy fibre terminals were found in the normal projection areas of granule cells, thus indicating that newly synthesized GAP-43 is transported predominantly in the axonal branches actively undergoing remodelling. These results provide evidence of the involvement of GAP-43 in the structural remodelling of mossy fibres, and suggest a role of this protein in the functional activity of the sprouted mossy fibres as a consequence of KA-induced seizures.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/00001756-199412000-00060</identifier><identifier>PMID: 7696623</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott-Raven Publishers</publisher><subject>Animals ; Biological and medical sciences ; Development. Senescence. Regeneration. Transplantation ; Fundamental and applied biological sciences. Psychology ; GAP-43 Protein ; Hippocampus - drug effects ; Hippocampus - metabolism ; Hippocampus - ultrastructure ; Immunohistochemistry ; Kainic Acid - pharmacology ; Male ; Membrane Glycoproteins - metabolism ; Microscopy, Electron ; Nerve Fibers - metabolism ; Nerve Fibers - ultrastructure ; Nerve Regeneration ; Nerve Tissue Proteins - metabolism ; Phosphoproteins - metabolism ; Rats ; Rats, Sprague-Dawley ; Tissue Distribution ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroreport, 1994-12, Vol.5 (18), p.2645-2648</ispartof><rights>Lippincott-Raven Publishers.</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4440-2cf7a38cb21b8789951c266ae27eb3f9d7f3d27066dd1b0ff0b3cec9d2fcc93b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3351952$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7696623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bendotti, Caterina</creatorcontrib><creatorcontrib>Baldessari, Sonia</creatorcontrib><creatorcontrib>Samanin, Rosario</creatorcontrib><creatorcontrib>Biasi, Silvia De</creatorcontrib><title>Ultrastructural immunolocalization of GAP-43 in the sprouted mossy fibres of kainic acid lesioned rats</title><title>Neuroreport</title><addtitle>Neuroreport</addtitle><description>KAINIC acid (KA)-induced seizures in adult rats have been reported to cause sprouting of mossy fibres in the inner molecular layer (IML) of the dentate gyrus. In the present study, using electron microscopic immunostaining for GAP-43 we found that 3 months after KA treatment, several GAP-43 positive terminals in the IML showed structural characteristics of mossy fibre boutons. No GAP-43-positive mossy fibre terminals were found in the normal projection areas of granule cells, thus indicating that newly synthesized GAP-43 is transported predominantly in the axonal branches actively undergoing remodelling. These results provide evidence of the involvement of GAP-43 in the structural remodelling of mossy fibres, and suggest a role of this protein in the functional activity of the sprouted mossy fibres as a consequence of KA-induced seizures.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAP-43 Protein</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - ultrastructure</subject><subject>Immunohistochemistry</subject><subject>Kainic Acid - pharmacology</subject><subject>Male</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Microscopy, Electron</subject><subject>Nerve Fibers - metabolism</subject><subject>Nerve Fibers - ultrastructure</subject><subject>Nerve Regeneration</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Phosphoproteins - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tissue Distribution</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl1LBCEUhiWKbfv4CYEX0d2UX6PjZURfENRFC92J4yhrOTOlDlG_Pmu3vYsEETnPezznvAIAMTrFSIozVBYWNa-wlAyTcqvK5mgLzDETtKrr5mkbzJGsZcUkr3fBXkrPBZEINzMwE1xyTugcuEXIUaccJ5OnqAP0fT8NYxiNDv5TZz8OcHTw-vyhYhT6Aealhek1jlO2HezHlD6g82206Rt70X7wBmrjOxhsKuICRZ3TAdhxOiR7uD73weLq8vHiprq7v769OL-rDGMMVcQ4oWljWoLbRjRS1tgQzrUlwrbUyU442hGBOO863CLnUEuNNbIjzhhJW7oPTlZ5S4Vvk01Z9T4ZG4Ie7DglJYSQVDTsXxBzLjBHvIDNCjSxNButU6_R9zp-KIzUtxfq1wu18UL9eFGkR-s3pra33Ua4Hn6JH6_jOpVxu6gH49MGo7TGsiYFYyvsfQzZxvQSpncb1dLqkJfqr59AvwCYbKIS</recordid><startdate>19941220</startdate><enddate>19941220</enddate><creator>Bendotti, Caterina</creator><creator>Baldessari, Sonia</creator><creator>Samanin, Rosario</creator><creator>Biasi, Silvia De</creator><general>Lippincott-Raven Publishers</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19941220</creationdate><title>Ultrastructural immunolocalization of GAP-43 in the sprouted mossy fibres of kainic acid lesioned rats</title><author>Bendotti, Caterina ; Baldessari, Sonia ; Samanin, Rosario ; Biasi, Silvia De</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4440-2cf7a38cb21b8789951c266ae27eb3f9d7f3d27066dd1b0ff0b3cec9d2fcc93b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAP-43 Protein</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - ultrastructure</topic><topic>Immunohistochemistry</topic><topic>Kainic Acid - pharmacology</topic><topic>Male</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Microscopy, Electron</topic><topic>Nerve Fibers - metabolism</topic><topic>Nerve Fibers - ultrastructure</topic><topic>Nerve Regeneration</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Phosphoproteins - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Tissue Distribution</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bendotti, Caterina</creatorcontrib><creatorcontrib>Baldessari, Sonia</creatorcontrib><creatorcontrib>Samanin, Rosario</creatorcontrib><creatorcontrib>Biasi, Silvia De</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bendotti, Caterina</au><au>Baldessari, Sonia</au><au>Samanin, Rosario</au><au>Biasi, Silvia De</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural immunolocalization of GAP-43 in the sprouted mossy fibres of kainic acid lesioned rats</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>1994-12-20</date><risdate>1994</risdate><volume>5</volume><issue>18</issue><spage>2645</spage><epage>2648</epage><pages>2645-2648</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>KAINIC acid (KA)-induced seizures in adult rats have been reported to cause sprouting of mossy fibres in the inner molecular layer (IML) of the dentate gyrus. In the present study, using electron microscopic immunostaining for GAP-43 we found that 3 months after KA treatment, several GAP-43 positive terminals in the IML showed structural characteristics of mossy fibre boutons. No GAP-43-positive mossy fibre terminals were found in the normal projection areas of granule cells, thus indicating that newly synthesized GAP-43 is transported predominantly in the axonal branches actively undergoing remodelling. These results provide evidence of the involvement of GAP-43 in the structural remodelling of mossy fibres, and suggest a role of this protein in the functional activity of the sprouted mossy fibres as a consequence of KA-induced seizures.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott-Raven Publishers</pub><pmid>7696623</pmid><doi>10.1097/00001756-199412000-00060</doi><tpages>4</tpages></addata></record> |
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| ispartof | Neuroreport, 1994-12, Vol.5 (18), p.2645-2648 |
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| subjects | Animals Biological and medical sciences Development. Senescence. Regeneration. Transplantation Fundamental and applied biological sciences. Psychology GAP-43 Protein Hippocampus - drug effects Hippocampus - metabolism Hippocampus - ultrastructure Immunohistochemistry Kainic Acid - pharmacology Male Membrane Glycoproteins - metabolism Microscopy, Electron Nerve Fibers - metabolism Nerve Fibers - ultrastructure Nerve Regeneration Nerve Tissue Proteins - metabolism Phosphoproteins - metabolism Rats Rats, Sprague-Dawley Tissue Distribution Vertebrates: nervous system and sense organs |
| title | Ultrastructural immunolocalization of GAP-43 in the sprouted mossy fibres of kainic acid lesioned rats |
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