Polymerase chain reaction for Toxoplasma gondii DNA in the cerebrospinal fluid of AIDS patients with focal brain lesions
To study the accuracy of polymerase chain reaction (PCR) for Toxoplasma gondii DNA in the cerebrospinal fluid (CSF) of AIDS patients for the diagnosis of T. gondii encephalitis. Eighty-two AIDS patients with brain lesions. At autopsy, 19 patients (group A) had toxoplasmic encephalitis and 33 (group...
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| Veröffentlicht in: | AIDS (London) 1994-12, Vol.8 (12), p.1691-1694 |
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| container_title | AIDS (London) |
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| creator | NOVATI, R CASTAGNA, A MORSICA, G VAGO, L TAMBUSSI, G GHEZZI, S GERVASONI, C BISSON, C D'ARMINIO MONFORTE, A LAZZARIN, A |
| description | To study the accuracy of polymerase chain reaction (PCR) for Toxoplasma gondii DNA in the cerebrospinal fluid (CSF) of AIDS patients for the diagnosis of T. gondii encephalitis.
Eighty-two AIDS patients with brain lesions. At autopsy, 19 patients (group A) had toxoplasmic encephalitis and 33 (group B) primary brain lymphoma or other infections. Brain histology was not available for 30 patients; cerebral lesions improved after anti-Toxoplasma therapy in 16 (group C), but there was no improvement in 14 patients (group D).
T. gondii RH strain was serially diluted in microplate wells. After heat denaturation, nested PCR was performed on diluted tachyzoites and on 10 microliters CSF with primers flanking the B1 repetitive region of T. gondii genome.
DNA from one to five tachyzoites was detected in each experiment. PCR was positive in eight (42.1%) out of 19 group A samples, none of the group B samples, 10 (62.5%) out of 16 group C samples and none of the group D samples. Among group A and C patients, PCR was positive in all 11, and in seven out of 24 (29.1%; P < 0.04) patients who had received anti-Toxoplasma therapy for less or more than 1 week at the time of rachicentesis, respectively.
Nested PCR for T. gondii in CSF may improve early differential diagnosis of AIDS-associated focal brain lesions. Higher diagnostic accuracy was achieved when lumbar puncture was performed in the first week of anti-Toxoplasma therapy. |
| doi_str_mv | 10.1097/00002030-199412000-00008 |
| format | Article |
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Eighty-two AIDS patients with brain lesions. At autopsy, 19 patients (group A) had toxoplasmic encephalitis and 33 (group B) primary brain lymphoma or other infections. Brain histology was not available for 30 patients; cerebral lesions improved after anti-Toxoplasma therapy in 16 (group C), but there was no improvement in 14 patients (group D).
T. gondii RH strain was serially diluted in microplate wells. After heat denaturation, nested PCR was performed on diluted tachyzoites and on 10 microliters CSF with primers flanking the B1 repetitive region of T. gondii genome.
DNA from one to five tachyzoites was detected in each experiment. PCR was positive in eight (42.1%) out of 19 group A samples, none of the group B samples, 10 (62.5%) out of 16 group C samples and none of the group D samples. Among group A and C patients, PCR was positive in all 11, and in seven out of 24 (29.1%; P < 0.04) patients who had received anti-Toxoplasma therapy for less or more than 1 week at the time of rachicentesis, respectively.
Nested PCR for T. gondii in CSF may improve early differential diagnosis of AIDS-associated focal brain lesions. Higher diagnostic accuracy was achieved when lumbar puncture was performed in the first week of anti-Toxoplasma therapy.</description><identifier>ISSN: 0269-9370</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/00002030-199412000-00008</identifier><identifier>PMID: 7888118</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>AIDS-Related Opportunistic Infections - complications ; AIDS-Related Opportunistic Infections - diagnosis ; AIDS-Related Opportunistic Infections - drug therapy ; AIDS/HIV ; Animals ; Base Sequence ; Biological and medical sciences ; Brain Neoplasms - complications ; Brain Neoplasms - diagnosis ; Coccidiostats - therapeutic use ; Diagnosis, Differential ; DNA Probes - genetics ; DNA, Protozoan - cerebrospinal fluid ; DNA, Protozoan - genetics ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Lymphoma - complications ; Lymphoma - diagnosis ; Medical sciences ; Molecular Sequence Data ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - statistics & numerical data ; Sensitivity and Specificity ; Toxoplasma - genetics ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; Toxoplasmosis, Cerebral - complications ; Toxoplasmosis, Cerebral - diagnosis ; Toxoplasmosis, Cerebral - drug therapy</subject><ispartof>AIDS (London), 1994-12, Vol.8 (12), p.1691-1694</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-335805f668c31b4eac416627a8fe37fbfec82b24325325914005f873fb4645393</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3337881$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7888118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NOVATI, R</creatorcontrib><creatorcontrib>CASTAGNA, A</creatorcontrib><creatorcontrib>MORSICA, G</creatorcontrib><creatorcontrib>VAGO, L</creatorcontrib><creatorcontrib>TAMBUSSI, G</creatorcontrib><creatorcontrib>GHEZZI, S</creatorcontrib><creatorcontrib>GERVASONI, C</creatorcontrib><creatorcontrib>BISSON, C</creatorcontrib><creatorcontrib>D'ARMINIO MONFORTE, A</creatorcontrib><creatorcontrib>LAZZARIN, A</creatorcontrib><title>Polymerase chain reaction for Toxoplasma gondii DNA in the cerebrospinal fluid of AIDS patients with focal brain lesions</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>To study the accuracy of polymerase chain reaction (PCR) for Toxoplasma gondii DNA in the cerebrospinal fluid (CSF) of AIDS patients for the diagnosis of T. gondii encephalitis.
Eighty-two AIDS patients with brain lesions. At autopsy, 19 patients (group A) had toxoplasmic encephalitis and 33 (group B) primary brain lymphoma or other infections. Brain histology was not available for 30 patients; cerebral lesions improved after anti-Toxoplasma therapy in 16 (group C), but there was no improvement in 14 patients (group D).
T. gondii RH strain was serially diluted in microplate wells. After heat denaturation, nested PCR was performed on diluted tachyzoites and on 10 microliters CSF with primers flanking the B1 repetitive region of T. gondii genome.
DNA from one to five tachyzoites was detected in each experiment. PCR was positive in eight (42.1%) out of 19 group A samples, none of the group B samples, 10 (62.5%) out of 16 group C samples and none of the group D samples. Among group A and C patients, PCR was positive in all 11, and in seven out of 24 (29.1%; P < 0.04) patients who had received anti-Toxoplasma therapy for less or more than 1 week at the time of rachicentesis, respectively.
Nested PCR for T. gondii in CSF may improve early differential diagnosis of AIDS-associated focal brain lesions. Higher diagnostic accuracy was achieved when lumbar puncture was performed in the first week of anti-Toxoplasma therapy.</description><subject>AIDS-Related Opportunistic Infections - complications</subject><subject>AIDS-Related Opportunistic Infections - diagnosis</subject><subject>AIDS-Related Opportunistic Infections - drug therapy</subject><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brain Neoplasms - complications</subject><subject>Brain Neoplasms - diagnosis</subject><subject>Coccidiostats - therapeutic use</subject><subject>Diagnosis, Differential</subject><subject>DNA Probes - genetics</subject><subject>DNA, Protozoan - cerebrospinal fluid</subject><subject>DNA, Protozoan - genetics</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Lymphoma - complications</subject><subject>Lymphoma - diagnosis</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - statistics & numerical data</subject><subject>Sensitivity and Specificity</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Cerebral - complications</subject><subject>Toxoplasmosis, Cerebral - diagnosis</subject><subject>Toxoplasmosis, Cerebral - drug therapy</subject><issn>0269-9370</issn><issn>1473-5571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVocB03P6GgQ-ltU2lnVx9H43yCaQJJzotWlmqV3dVWWpPk32fcuL5WCIRmnnk1o5cQytkFZ1r-YLhKBqzgWle8xFuxD6kTMueVhKKuJf9E5qwUutAg2WdylvNvJGqm1IzMpFKKczUnrw-xe-tdMtlRuzVhoMkZO4U4UB8TfYqvcexM7g39FYdNCPTy55IiNW2Rd8m1KeYxDKajvtuFDY2eLu8uH-lopuCGKdOXMG1RyiLRpr1-5zKq5y_k1Jsuu_PDuSDP11dPq9tifX9zt1quC4ttTwVArVjthVAWeFthbxUXopRGeQfSt95ZVbZlBWWNW_MKR_RKgm8rUdWgYUG-f-iOKf7ZuTw1fcjWdZ0ZXNzlRkqpgUnxX5ALCYJphaD6AC3OnpPzzZhCb9Jbw1mzd6f5505zdOdvaF_69fDGru3d5lh4sAPz3w55k_HHfDKDDfmIAQCSHN4BjOiWkQ</recordid><startdate>19941201</startdate><enddate>19941201</enddate><creator>NOVATI, R</creator><creator>CASTAGNA, A</creator><creator>MORSICA, G</creator><creator>VAGO, L</creator><creator>TAMBUSSI, G</creator><creator>GHEZZI, S</creator><creator>GERVASONI, C</creator><creator>BISSON, C</creator><creator>D'ARMINIO MONFORTE, A</creator><creator>LAZZARIN, A</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19941201</creationdate><title>Polymerase chain reaction for Toxoplasma gondii DNA in the cerebrospinal fluid of AIDS patients with focal brain lesions</title><author>NOVATI, R ; CASTAGNA, A ; MORSICA, G ; VAGO, L ; TAMBUSSI, G ; GHEZZI, S ; GERVASONI, C ; BISSON, C ; D'ARMINIO MONFORTE, A ; LAZZARIN, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-335805f668c31b4eac416627a8fe37fbfec82b24325325914005f873fb4645393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>AIDS-Related Opportunistic Infections - complications</topic><topic>AIDS-Related Opportunistic Infections - diagnosis</topic><topic>AIDS-Related Opportunistic Infections - drug therapy</topic><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Brain Neoplasms - complications</topic><topic>Brain Neoplasms - diagnosis</topic><topic>Coccidiostats - therapeutic use</topic><topic>Diagnosis, Differential</topic><topic>DNA Probes - genetics</topic><topic>DNA, Protozoan - cerebrospinal fluid</topic><topic>DNA, Protozoan - genetics</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Lymphoma - complications</topic><topic>Lymphoma - diagnosis</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - statistics & numerical data</topic><topic>Sensitivity and Specificity</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Cerebral - complications</topic><topic>Toxoplasmosis, Cerebral - diagnosis</topic><topic>Toxoplasmosis, Cerebral - drug therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NOVATI, R</creatorcontrib><creatorcontrib>CASTAGNA, A</creatorcontrib><creatorcontrib>MORSICA, G</creatorcontrib><creatorcontrib>VAGO, L</creatorcontrib><creatorcontrib>TAMBUSSI, G</creatorcontrib><creatorcontrib>GHEZZI, S</creatorcontrib><creatorcontrib>GERVASONI, C</creatorcontrib><creatorcontrib>BISSON, C</creatorcontrib><creatorcontrib>D'ARMINIO MONFORTE, A</creatorcontrib><creatorcontrib>LAZZARIN, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NOVATI, R</au><au>CASTAGNA, A</au><au>MORSICA, G</au><au>VAGO, L</au><au>TAMBUSSI, G</au><au>GHEZZI, S</au><au>GERVASONI, C</au><au>BISSON, C</au><au>D'ARMINIO MONFORTE, A</au><au>LAZZARIN, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase chain reaction for Toxoplasma gondii DNA in the cerebrospinal fluid of AIDS patients with focal brain lesions</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>1994-12-01</date><risdate>1994</risdate><volume>8</volume><issue>12</issue><spage>1691</spage><epage>1694</epage><pages>1691-1694</pages><issn>0269-9370</issn><eissn>1473-5571</eissn><abstract>To study the accuracy of polymerase chain reaction (PCR) for Toxoplasma gondii DNA in the cerebrospinal fluid (CSF) of AIDS patients for the diagnosis of T. gondii encephalitis.
Eighty-two AIDS patients with brain lesions. At autopsy, 19 patients (group A) had toxoplasmic encephalitis and 33 (group B) primary brain lymphoma or other infections. Brain histology was not available for 30 patients; cerebral lesions improved after anti-Toxoplasma therapy in 16 (group C), but there was no improvement in 14 patients (group D).
T. gondii RH strain was serially diluted in microplate wells. After heat denaturation, nested PCR was performed on diluted tachyzoites and on 10 microliters CSF with primers flanking the B1 repetitive region of T. gondii genome.
DNA from one to five tachyzoites was detected in each experiment. PCR was positive in eight (42.1%) out of 19 group A samples, none of the group B samples, 10 (62.5%) out of 16 group C samples and none of the group D samples. Among group A and C patients, PCR was positive in all 11, and in seven out of 24 (29.1%; P < 0.04) patients who had received anti-Toxoplasma therapy for less or more than 1 week at the time of rachicentesis, respectively.
Nested PCR for T. gondii in CSF may improve early differential diagnosis of AIDS-associated focal brain lesions. Higher diagnostic accuracy was achieved when lumbar puncture was performed in the first week of anti-Toxoplasma therapy.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>7888118</pmid><doi>10.1097/00002030-199412000-00008</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0269-9370 |
| ispartof | AIDS (London), 1994-12, Vol.8 (12), p.1691-1694 |
| issn | 0269-9370 1473-5571 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | AIDS-Related Opportunistic Infections - complications AIDS-Related Opportunistic Infections - diagnosis AIDS-Related Opportunistic Infections - drug therapy AIDS/HIV Animals Base Sequence Biological and medical sciences Brain Neoplasms - complications Brain Neoplasms - diagnosis Coccidiostats - therapeutic use Diagnosis, Differential DNA Probes - genetics DNA, Protozoan - cerebrospinal fluid DNA, Protozoan - genetics Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Lymphoma - complications Lymphoma - diagnosis Medical sciences Molecular Sequence Data Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data Sensitivity and Specificity Toxoplasma - genetics Toxoplasma - isolation & purification Toxoplasma gondii Toxoplasmosis, Cerebral - complications Toxoplasmosis, Cerebral - diagnosis Toxoplasmosis, Cerebral - drug therapy |
| title | Polymerase chain reaction for Toxoplasma gondii DNA in the cerebrospinal fluid of AIDS patients with focal brain lesions |
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